Posted by admin on Sep 30, 2025 in |
Antimicrobials are vital agents that combat dangerous microorganisms, but increasing resistance to these drugs is a major challenge to global health security. AMR occurs when the intended killing drugs lose their effectiveness because pathogens have learned how to adapt and become resistant to them mainly as a result of overusing, misusing or using antibiotics in ways that are not suitable. Adhering with this pressing matter necessitates fresh methods coupled with an all-inclusive perspective. Literature was searched through indexed databases including Scopus, PubMed, Google Scholar and Science director. Research on novel AMR treatments is continuing. Anti-virulent therapy targets bacteria’s virulence factors rather than eradicating them, preventing antibiotic-resistant strains. Naturally occurring or manufactured antimicrobial peptides have diverse modes of action and little resistance risk. Using antimicrobials to prevent infections reduces the need for curative antibiotics. Bacteriophage therapy employs viruses that infect and kill bacteria to treat illnesses. Plant derivatives like phytochemicals and nanoparticles are antimicrobial.Review addressing the challenge of antimicrobial resistance requirements and for multi-pronged approach, including surveillance, stewardship and development...
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Posted by admin on Jul 31, 2025 in |
The aim of this study is to assess the antibacterial efficacy of the Datura stramonium seeds and leaves extracts against Mycobacterium kansasii. The antimicrobial experiments were performed using five doses (0.25µg/ml, 0.50µg/ml, 1µg/ml, 2µg/ml, 4µg/ml) of seeds and leaves extracts. The results showed notable inhibitory effects on Mycobacterium kansasii. The results indicated that the extract of D. stramonium exhibited action against all the tested bacterial isolates, with the level of activity varying depending upon the concentration. The hexane extract of seeds DSS-1H exhibited antibacterial activity, with mean zones of inhibition measuring 98.94±50.25.%, 120.98±56.92%, 120.499±69.60%, 125.752±133.18% and 89.494±180.63%, at the doses of 0.25, 0.50, 1, 2, and 4 µg/ml and while treating with DSS-2A the percent cell viability of the microbial cell was found to be 128.02±25.64%, 86.79±14.95%, 34.61±16.33%, 26.29±29.76% and 32.56±19.48% at the doses of 0.25, 0.50, 1, 2 and 4µg/ml, respectively, with incubation period of 48 hours. Similarly, the microbial cells were treated (0.25, 0.50,1,2,4 µg/ml) with DSS-3ET with incubation period of 48 hours, indicating a more substantial...
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Posted by admin on May 31, 2025 in |
Antimicrobial resistance continues to cause antibiotics treatment failure and millions of deaths globally. Inhibition of a double-stranded binding protein-A enzyme (DsbA), machinery in bacteria resistance and pathogenesis mechanisms make the bacteria vulnerable to existing antibiotics, allowing restoration of antimicrobial efficacy. However, there is a gap in identifying bioactive and safe DsbA-inhibitors as a therapeutic restoration strategy. In this in-silico screening study, a co-crystallized E. coli-DsbA enzyme protein was used as the target. Seven bioactive ligands: linoleic acid, aloesin, azadirachtin, nimbin, palmitic acid, stearic acid, and aloeresin-A, showed maximum docking scores of low binding affinity (kcal/mol): -6.8345, – 5.9434, – 7.2810, -6.2394, -6.5958, -6.7170 and -6.9554 respectively, lower than the standard (9AG): -4. 6919. The ligands obeyed Lipinski’s rule of 5. The Root Mean Square Deviation (RMSD) of E. coli-DsbA complexes with azadirachtin, and aloeresin-A displayed remarkable stability throughout the simulations. Molecular dynamics simulation on azadirachtin and aloeresin-A showed great docking scores and modes over 10000ps and revealed lowest binding affinity scores, remain stable in the active site. Additionally, astructural-activity...
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Posted by admin on May 31, 2025 in |
The current study aims to find out how effective different extractions of Barleria cristata leaves are at fighting cancer, free radicals, and bacteria. Physical and chemical qualities and preliminary phytochemical analysis will also be assessed. It also quantifies several active ingredients by total phenolic and flavonoid. Five leaf extracts of Barleria cristata were made with ethyl acetate, chloroform, acetone, ethanol, and water. They were then tested for their antioxidant potential using reducing power and free radical scavenging methods, such as DPPH and NO radicals. They were also tested for antibacterial activity against Gram-positive and Gram-negative bacteria. The MTT assay measured how successfully the medication killed MCF-7 breast cancer cell line and Hep-G2 hepatoma cancer cell line. We calculated IC50. Initial phytochemical analysis was performed on all extracts. The total extract was abundant in protein, carbs, tannins, flavonoids, and phenolics. Acetone extract had higher phenolic and flavonoid components than ethyl acetate, chloroform, ethanol, and water. Barleria cristata acetone extract was antioxidant rich. Plant extracts also had antibacterial properties. Extracts inhibited...
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Posted by admin on Apr 30, 2025 in |
Background: The emergence and spread of Vancomycin, Linezolid and combined linezolid/vancomycin resistance in Enterococcus species (LVRE) is a major therapeutic challenge. Vancomycin-resistant Enterococci are an important cause of healthcare-associated infections. Linezolid and Teicoplanin are currently approved for the treatment of VRE; however, resistance to these antimicrobials appears to be increasing. Aims/Objectives: The study was done to find the prevalence and to study the antimicrobial resistance pattern of Enterococci isolated from patients with bacteremia in a tertiary care hospital in Ahmedabad. Methodology: This retrospective study was conducted for a period of one year; Blood samples were received and incubated in Bact/Alert 3D automated blood culture machine. Positive indicated blood samples were cultured, and identification and antimicrobial susceptibility testing was done using both manual and automated methods. Results: In this study, 4147(35%) blood samples were found positive. Out of 4147 positive blood samples 278(6.7%) samples were shown growth with Enterococci species. Out of 278 Enterococci, 8(2.87%) Enterococci were found to have both linezolid and vancomycin resistance. Conclusions: Prevalence of LVRE is low...
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