Posted by admin on Nov 30, 2019 in |
Quantitative structure relationship techniques (QSRT) is among the most widely used computational technology for analog-based drug design. A molecular modeling approach using theoretical, computational analysis through chem sketch ACD lab online software was done for determination of probable antimicrobial activity. To develop a pharmacophoric model for inhibition, QSRT parameters PCP, ADME, and toxicity has been used. BCF (Bioconcentration factor), adsorption coefficient, log P, log D values, plasma protein binding, P-gp inhibition, AMES test, hERg inhibition, estrogen receptor, and Lipinski’s type properties have been calculated. From a data set of 11 analogs, it has been concluded that all compounds are non-bio-accumulative, non-endocrine disruptors, non hERg inhibitors, genotoxic and within Lipinski’s criteria. None of the compounds are practically ionizable at various body pH values, and all are zwitterionic compounds. From these data and in-vitro antimicrobial data compounds, 5c and 5f can be exploited for the formulation of bactericide and fungicide with a slight modification in their...
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Posted by admin on Nov 30, 2019 in |
Purpose: Helicobacter pylori (H-pylori) infection has been commonly associated with gastric ulcers. Lepidium sativum (L. Sativum) is an herbaceous edible plant, botanically related to mustard and watercress with remarkable pharmacological features. In this study, we investigate the anti-microbial and anti-oxidant effect of L. sativum seed methanolic extract in-vitro and correlate this with the gastroprotective potential of this extract against H-pylori–induced gastric ulcer in an in-vivo model. Methods: L. sativum extract was quantified for phenolic compounds using high-performance liquid chromatography, and the anti-microbial and anti-oxidant activities of the extract were evaluated in-vitro. Furthermore, an in-vivo rat model was assessed in order to explore the effect of L. sativum extract against H. pylori-induced gastric ulcer in-vitro. Results: The L. sativum extract exhibited significant antioxidant and anti-microbial activities against different pathogenic organisms. In vivo, L. sativum extract reduced the ulcer index, increased the percentage of ulcer inhibition, halted lipid peroxide and triggered anti-oxidant markers. Moreover, it suppressed inflammatory and apoptotic markers. The histopathological results for L. sativum treated rats showed the amelioration...
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Posted by admin on Nov 30, 2019 in |
Piper sarmentosum Roxb. belongs to the genus Piper in which traditional application described as remedy for several diseases. Considering its vast potential as effective pharmacological agent, a study was conducted to assess the phytochemical and biological activities of P. sarmentosum leaf extracts, with particular focus on their anti-microbial, anti-oxidant and anticancer properties. Results for antimicrobial test revealed that it has positive activity against the tested microorganisms namely, Bacillus subtilis and Klebsiella pneumonia. In terms of the antioxidant activity of the extracts, using DPPH scavenging assay, it can be observed that P. sarmentosum has an IC50 of 55.25 ppm, suggesting a potentially significant source of antioxidants. The extracts also exhibited a strong cytotoxic activities against three human cancer cell lines; cervical carcinoma (HeLa), breast cancer (MCF-7), and colon adenocarcinoma (HT-29). The antimicrobial and antioxidant activities of the leaf extract could be attributed to the tannins and flavonoids identified using phytochemical analysis together with steroids and alkaloids. Further analysis of the bioactive compounds using GC-MS analysis revealed the presence of phytochemicals...
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Posted by admin on Oct 31, 2019 in |
The desire of the present study was to investigate in-vitro antimicrobial activity on different crude extracts of Ziziphus rugosa pericarp. The antibacterial assay was carried out against Bacillus subtilis MTCC 1133, Staphylococcus aureus MTCC 7443, Escherichia coli MTCC 1692 and Salmonella typhi ATCC 19430. Meanwhile, Candida albicans ATCC 18804, Aspergillus niger ATCC 16404, Trichoderma harzianum MTCC 3832 and Polyporus rubidus MTCC 140 were used for the antifungal assay. Both activities were carried out by cup plate method using 100, 200, and 300mg/ml of hexane, ethanol, and aqueous extracts. The hexane extract showed MIC at 100 mg/ml for B. subtilis, 60 mg/ml for S. aureus and S. typhi, and 80 mg/ml for E. coli. The ethanolic extract showed 80 mg/ml for all tested organisms. The aqueous extract showed 80, 40, 60, and 100 mg/ml for B. subtilis, S. aureus, E. coli, and S. typhi, respectively. Among the extracts, aqueous extracts showed highest level activity against both gm +ve (B. subtilis 26mm) and gm -ve (S. typhi 24mm) at 300 mg/ml....
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Posted by admin on Oct 31, 2019 in |
Metal (II) complexes of Co, Ni and Cu with Schiff base derived from 2, 4-Dihydroxy-5-acetylacetophenone and Aminothiophenol (DAAAP) were synthesized and characterized by elemental analysis, FTIR, IH NMR, electronic spectra, thermal analysis, magnetic susceptibility and conductometric measurements. On the basis of these studies, a six coordinated octahedral geometry has been proposed for all the complexes. The Schiff base and its complexes were tested for biological activity against various types of Gram-positive (Bacillus subtilis ATTC 6051 and S. pyogones ATTC 12600) and Gram – negative bacteria (Escherichia coli ATTC 11775 and Proteus vulgaris ATTC 13315) and Fusarium solani Martius and Aspergillus niger Fungus. It has been found that metal complexes are found to have sensitivity for inhibition of Gram‐positive more than Gram‐negative bacteria. All the metal complexes show antifungal activity and it is clear from the data that the complexes exhibit more antifungal activity compared to the parent...
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