Posted by admin on Nov 30, 2017 in |
Background: Antimicrobial resistance against S.aureus infections is a global public health problem resulting in very limited treatment options, and inappropriate use of antibiotics is one of the most important factors that could affect the increasing pattern of resistance. Objective: To determine the antimicrobial resistance pattern of S. aureus among healthy and adult students to commonly used antimicrobial agents. Materials and Methods: This study was carried out at Cytogene Research and Development laboratory, Indira Nagar, Lucknow, India. Samples from throat were collected as throat-swab which were cultured and screened for S. aureus using standard microbiological protocols. The antimicrobial susceptibility of identified S. aureus was evaluated using disc diffusion technique. Results: A total of 47(54%) S. aureus were identified from 87growths of the 185throat swab samples. All the S. aureus isolates were methicillin resistant; they exhibited total resistance to penicillin, 100% to Erythromycin, 97.8%to Amoxicillin, 91.4% to Clindamycin, 78.7% to Vancomycin, 53.1% to Gentamicin, 44.6% to Trimethoprime, 19.1% to Tetracycline and 6.3% to Ciprofloxacin. Conclusion: The resistance to antibiotics agents is...
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Posted by admin on Nov 30, 2017 in |
The present work deals with formulation of topical gels containing aqueous extract of P. acerifolium seeds and also to evaluate the prepared gels against some Gram-positive and Gram-negative bacteria. The antibacterial activity of various P. acerifolium seed extracts was measured through determining minimum inhibitory concentration (MIC) by cube dilution method and zone of inhibition by disc diffusion method against E. coli, P. aeruginosa and S. aureus. Aqueous P. acerifolium seed extracts exhibited comparatively higher antibacterial potential in comparison with that of other extracts and employed to formulate topical herbal gels using 4.5 % sodium carboxymethyl cellulose as gel base. Prepared herbal gels were tested for pH, viscosity, extrudability and spreadability. All the prepared gels were subjected to antibacterial study by determining zone of inhibition by disc diffusion method. Herbal gels containing 15 % aqueous P. acerifolium seed extract exhibited better zones of inhibition against E. coli, P. aeruginosa and S. aureus in comparison with that of 5 % and 10 %. From the results of this study, it can...
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Posted by admin on Oct 31, 2017 in |
The present work deals with formulation of topical gels containing aqueous extract of P. acerifolium seeds and also evaluation of prepared gels against some Gram-positive and Gram-negative bacteria. The antibacterial activity of various P. acerifolium seed extracts was measured through determining minimum inhibitory concentration (MIC) by cube dilution method and zone of inhibition by disc diffusion method against E. coli, P. aeruginosa and S. aureus. Aqueous P. acerifolium seed extracts exhibited comparatively higher antibacterial potential in comparison with that of other extracts and employed to formulate topical herbal gels using 4.5% sodium carboxymethyl cellulose as gel base. Prepared herbal gels were tested for pH, viscosity, extrudability and spreadability. All the prepared gels were subjected to antibacterial study by determining zone of inhibition by disc diffusion method. Herbal gels containing 15% aqueous P. acerifolium seed extract exhibited better zones of inhibition against E. coli, P. aeruginosa and S. aureus in comparison with that of 7.5% and 10%. From the results of this study, it can be concluded that herbal gels...
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Posted by admin on Oct 31, 2017 in |
In the present study, the inhibitory effect of crude ethanolic extracts from four plants viz. Bauhinia racemosa Lamk (stem bark and roots), Holoptelea integrifolia (Roxb.) Planch. (roots), Kirganelia reticulata (Poir) Baill. (Stem bark) and Phyllanthus maderaspatensis Linn. (whole plant) were undertaken against some test bacteria and test fungi using Disc Diffusion Method. The screening data indicated that the antibacterial activity of the ethanolic extracts of B. racemosa (stem bark and root) inhibited the growth of S. aureus and P. vulgaris and H. integrifolia (roots) was found active against S. paratyphi B. The other two plants were totally inactive in this respect. A comparative study of the fungicidal activity of the four plant species indicated that although all of them exhibited activity against the select test fungi out of which the activity against A. flavus was most significant. Roots of B. racemosa were significantly active against A. niger, F. moniliforme and stem bark was active against R. bataticola. These could be attributed to the presence of racemosol and its derivatives...
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Posted by admin on Sep 30, 2017 in |
Nanotechnology is an innovative and escalating field that has made its contribution to all spheres of human life. Conventional synthesis of nanoparticles suffers from serious problems like high temperature, poor yield, longer reaction time, and use of toxic and highly sensitive compounds. Green synthesis of nanoparticles is an emerging field which is known to eliminate / minimize the aforementioned drawbacks. In the present investigation, stable silver nanoparticles were synthesized in good yield by chemical reduction of silver nitrate in ionic liquids such as [BMIM] BF4 (IL-1), [BMIM] PF6 (IL-2), [BEMIM] BF4 (IL-3) and [BEMIM] PF6 (IL-4) at room temperature. X-ray diffraction (XRD) and scanning electron microscopy (SEM) have been employed for the characterization of structure and morphology of the synthesized silver nanoparticles. The as synthesized nanoparticles and pure ionic liquids were screened for their anti-microbial activities and it was found that both the pure ILs and silver nanoparticles possess significant antimicrobial...
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