Posted by admin on Jul 31, 2017 in |
The present study is based on the comparative evaluation of preliminary phytochemical screening and in vitro antimicrobial activity of methanolic and ethanolic extracts of Stevia rebaudiana leaves. The crude extracts of S. rebaudiana leaves were subjected to phytochemical screening tests by using standard procedures for the detection of the presence or absence of eight major secondary metabolites. The free radical scavenging activity of both the extracts was measured by using the 1,1- diphenyl-2-picrylhydrazyl (DPPH) assay. The dose dependent increase was observed in DPPH activity of methanolic and ethanolic extracts (10, 20, 40, 50, 100 µg/ml), and the range was 28.65 – 64.35 % and 28.15 – 61.57 % as compared to ascorbic acid 30.15 – 85.99 %. The antibacterial activity of S. rebaudiana leaves was tested by agar-well diffusion assay. The plant extracts of four different concentrations (25, 50, 75 and 100 mg/mL) were prepared in DMSO and tested against eight bacterial strains and was compared with that of standard drug tetracycline (10 µg/mL). The results of preliminary phytochemical...
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Posted by admin on Jul 31, 2017 in |
The present study was conducted to evaluate the antioxidant and antibacterial activities of methanol and aqueous extracts of whole plant of Hydrocotyle sibthorpiodes Lam. Antioxidant activity was measured using reducing power, hydrogen peroxide and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. The antioxidant activity was expressed as percentage (%) of DPPH and H2O2 radicals’ inhibition and IC50 value. Methanolic extract of the whole plant showed highest antioxidant activity each for DPPH (85.80±0.29) as well as H2O2 (64.7±0.173) compared to the aqueous extract with an IC50 value of 50.1µg/ml and 60.2 µg/ml respectively. Antibacterial activity was tested against two pathogenic strains by agar well diffusion method. The extracts were found to exert low to moderate antibacterial activity compared to Chloramphenicol which was taken as...
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Posted by admin on Jul 31, 2017 in |
Peptides are recognized for being highly selective efficacious and at the same time, relatively safe and well tolerated. Consequently, there is an increased interest in peptides, in pharmaceutical research and development (R&D) and approximately 140 peptide therapeutics are currently being evaluated in clinical trials. Ultrasonic is an area of intense scientific and technological research, science and technology of ultrasonics are widely used in the recent years for industrial and medical applications. Ultrasonic investigation in non- aqueous solutions of electrolytes and non- electrolytes with amino acids provide useful information in understanding the behaviour of liquid/solution system. In the present work, the ultrasonic velocity of a peptide with drug in formamide have been measured at various concentrations and at different temperatures. Using the measured value the thermodynamic parameters and acoustical parameters are calculated. These parameters have been utilized to study the interionic and intermolecular interactions existing in the system. The results arrived from ultrasonic methods have been corroborated with antimicrobial...
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Posted by admin on Jun 30, 2017 in |
In this research work Zn1-xAgxO1-δ (where x = 0.05, 0.10, 0.15 and 0.20) nano powders were synthesized via simple soft chemical method using corresponding metal nitrates and alkali precipitant. Sodium hydroxide was used as a precipitant during the synthesis. The prepared samples were characterized using X-ray diffraction, FTIR spectroscopy, particle size analysis, SEM, EDAX analysis and TEM. The antimicrobial activity of the silver doped zinc oxide nanoparticles were studied against both Gram positive (Staphylococcus aureus and Bacillus subtilis) and Gram negative (Escherichia coli and Klebsiella pneumoniae) bacteria by agar well diffusion method. Considering the better antimicrobial properties obtained with Zn0.80Ag0.20O1-δ, these materials were subjected for in vivo acute oral nanotoxicological studies in Swiss Albino mice. The histopathological changes in livers and kidneys of Swiss Albino mice due to the oral ingestion of the above material (Zn0.80Ag0.20O1-δ) at different dosage levels on day 14 administration have shown the LD50 as greater than 2000 mg/kg body...
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Posted by admin on Jun 30, 2017 in |
Acid amine coupling of 1H-indol-6-yl(piperazin-1-yl) methanone with different acids has been synthesized in presence of HATU reagent, DIPEA base with DMF solvent at 45 °C 30 mins to yield Indole piperzine derivatives 3a-j. The structures of all the synthesized compounds have been characterised by elemental analysis and spectral studies like 1H-NMR and LCMS. Some selected Indole piperazine derivatives have been tested for antimicrobial activity using by Disc diffusion method and in vitro antioxidant activity using DPPH Assay method. Three compounds 3a, 3f and 3h have shown significant antimicrobial and one compound 3b have shown in vitro antioxidant...
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