Posted by admin on Mar 31, 2016 in |
Diabetic foot ulcer (DFU) is one of the major problems encountered by nearly 15 % of diabetic patients. Combinations of different antibiotics are used to treat the infection which makes the microorganism multi drug resistant. Among various types of drug delivery system, transdermal drug delivery is preferred in treating DFU because it delivers the drug directly to the wound site, which increases the rate of healing. Terminalia chebula (King of medicine) is known to possess wound healing ability and high antimicrobial activity against almost all bacteria isolated from DFU. In this scenario, we have fabricated different combinations of drug loaded composite films of cellulose acetate and chitosan. The formulated films were characterized using UV-visible and FT-IR spectrophotometer. Physicochemical characterization of the films was done with respect to drug uniformity, moisture uptake, folding endurance, drug release and antimicrobial studies. The antimicrobial activity of the films was evaluated for E.coli and B.subtilis and observed a good control of the growth of microorganism. The results suggest that the cellulose based films are...
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Posted by admin on Mar 1, 2016 in |
A freshwater microalga, Nostoc sp. isolated from Tamdil Wetland of Mizoram was tested for its algicidal, antibacterial and antifungal activity. Extracts were prepared with methanol, ethanol:water and dichloromethane:isopropanol and tested for their antimicrobial activity against nine microorganisms comprising of three chlorophycean algae (Chlorella vulgaris, Scenedesmus quadricauda and Selenastrum capricornatum), one cyanobacterium (Anabaena variabilis), three bacterial strains (Bacillus subtilis, Bacillus pumilus and Escherichia coli) and two fungal strains (Fusarium udum and Fusarium culmorum). Among the three extracts tested the methanol extract was found most effective on algal test organisms. The highest (60%) inhibitory effect was observed against A. variabilis by methanol extract followed by 31% inhibition by dichloromethane:isopropanol. When tested on bacterial strains, the highest inhibition zone (23.67±1.58mm) was observed in B. subtilis by dichloromethane:isopropanol extract followed by ethanol:water extract. In case of fungal activity, the highest inhibition zone (11.00±0.58mm) was observed in F. culmorum by dichloromethane:isopropanol extract and the rest of the extracts showed almost similar effect. MICs of each extract on all the organisms varied. The extracts were...
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Posted by admin on Jan 31, 2016 in |
A galactose-specific lectin was purified from seeds of a leguminous plant, Bauhinia variegata, by affinity chromatography on lactose–agarose. Protein extract agglutinated human erythrocytes (treated with proteolytic enzyme). Among various carbohydrates tested, the lectin was best inhibited by D-galactose and other sacharides. SDS-PAGE showed that the lectin, named BVL produce a single band establishing that the lectin is composed of similar type of subunits. The antimicrobial activity of the purified lectin was carried out by agar ditch diffusion method at different concentrations using appropriate standards. BVL demonstrated a remarkable antibacterial activity against the pathogenic bacteria Pseudomonas aeroginosa, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. BVL also shows a significant antifungal activity against Aspergilas niger and Penicilium...
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Posted by admin on Dec 31, 2015 in |
Many microorganisms thrive on the abundant nutrients in the rhizosphere and they produce antagonistic metabolites. The present study is an attempt to investigate the potent bacteria producing antibiotics against pathogenic microorganisms from the rhizosphere soil samples of Kanjamalai hills, Salem district, Tamilnadu, India. A total of 6 isolates of bacteria was obtained and screened for inhibitory activity against selected opportunistic pathogens. Potent bacterial isolate (A6) was identified as Pseudomonas aeruginosa by cultural, morphological and biochemical characterization. The antimicrobial activity of broth culture of Pseudomanas aeruginosa was also observed. The antimicrobial active compound was partially purified from the broth culture and its concentration was estimated to be 373.086 µg/ml. The obtained protein was separated in SDS-PAGE and its molecular weight was determined as 66.4 kD, 44.3 kD, 29.0 kD, 20.1 kD and 14.3 kD using standard molecular markers. The results showed that the isolated strain has high potential against Bacillus megaterium and Aspergillus niger. It can be further investigated to identify the antibacterial compounds produced and their mechanisms of...
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Posted by admin on Dec 31, 2015 in |
In Ayurveda, there are less toxic herbal drugs known as upavisha. Upavisha are mostly use in various diseases with many therapeutically preparations. As per Ayurveda, Upavisha is group of less toxic drug. These drugs are either not having all ten gunas or they have less potent gunas. The properties of these drugs are to cure acute and chronic disease. These groups also have antimicrobial/ anti- infective activity. Ayurvedic formulation having these drugs can be used in various microbial disorders. The classical preparations of all upavisha are indicated in mostly chronic ailments, vatavikara, neuromuscular disorder, gastrointestinal disorder and skin...
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