Posted by admin on Nov 1, 2014 in |
The increasing resistance of microbes to conventional drugs has promoted the scientists to search for new biocides with broad activity. Plant derived essential oils (EOs) have been known to show antimicrobial activity against specific pathogens and therefore could be considered as alternative antimicrobial agents in controlling pathogens. The present study was done to investigate the antimicrobial activity of soybean, rice, mustard, olive, coconut and castor oil against 24 isolates of 6 different types of food borne pathogens. Antibacterial activities of essential oils were determined using agar disc diffusion assay. All EOs showed antibacterial activity against Escherichia coli, Pseudomonas sp., Staphylococcus aureus, Vibrio cholerae, Klebsiella spp., and Salmonella spp. Staphylococcus aureus was found more sensitive to soybean and mustard oils compared to other Gram negative bacteria. Castor oil was effective against most of the bacteria namely Escherichia coli, Staphylococcus aureus, Vibrio cholerae, Klebsiella spp. and Salmonella spp. The antibacterial activity of EOs could be used for treatment of bacterial infection and preservation of...
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Posted by admin on Oct 1, 2014 in |
Lepidium sativum Linn. locally known as ‘fetto’ is a fast-growing edible herb which belongs to the family Brassicaceae is traditionally used for the treatment of various human ailments including cold, headache, colic, abdominal pain, dysentery, swellings and aphrodisiac in Ethiopia. Phytochemical screening and antimicrobial activities were conducted on seeds of Lepidium sativium grown in Ethiopia. Qualitative phytochemical screening test of chloroform/ methanol crude extract showed the presence of phytochemicals: flavonoid, cholesterol, terpenoids, steroids, carbohydrates, glycosides, tannins, alkaloids, phenols, phytosterols, proteins, and saponins. Antimicrobial activities of crude extract were tested against four bacteria: two Gram-negative bacteria (Escherichia coli and Salmonella typhi) and two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtillis) and three fungi (Aspergillus niger, Fusarium oxysporum, and Fusarium solani). The crude extracts of the seeds of the plant were active against the tested bacteria and fungi. The antimicrobial activities of plant seeds crude extract were compared with that of chloramphenicol against bacteria and bavistin against fungi as reference antibiotics. The ethnopharmacological knowledge of Lepidium sativum was documented. It was...
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Posted by admin on Oct 1, 2014 in |
In recent years, a large number of oils and their constituents have been investigated for their antimicrobial properties against bacteria and fungi. The present investigation evaluated the antimicrobial potential of edible medicinal oils of Cocos nucifera, Helianthus annus, Brassica juncea, Ricinus communis, Arachis hypogea, Glycine max, Gossypium hirsutum and Sesamum indicum extracted from different solvents. There is a basis for the traditional use of these plants for local health remedies. Oils of these plants were screened for their antimicrobial activity against human pathogenic bacteria and fungi, causing skin diseases. The antimicrobial activity of these oils was investigated against Escherichia coli, Trichophyton rubrum and Candida albicans by agar well diffusion method, Helianthus annus seed oil showed maximum antimicrobial activity against tested bacteria and fungi with MIC values ranging from 0.62 to 40 mg/mL using inhibitory zone estimation. These results support the edible medicinal oils can be used to cure skin...
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Posted by admin on Oct 1, 2014 in |
Four wild plant species (Chrysthanthemum cornarium, Capparis spinosa, Carthamus lanatus, Haplophyllum tuberculatum) were investigated for their antioxidant activity, phenolic and flavonoid contents of their methanolic extracts. The antioxidant activity of the extracts was assessed their free radical scavenging properties using DPPH. Total phenolics and total flavonoids were detected by spectrophotometric and HPLC methods. The HPLC analysis compared with 14 reference standard phenolic compounds, results confirmed that gallic acid, chlorogenic acid, caffeic acid, 2,5- dihydroxy benzoic acid, and catechin were detected in the four methanolic plant extracts. HPLC chromatograms of the plant extracts showed that there was noticeable valuation in the distribution of phenolic compounds in the four studied species. Six bacterial strains were used in this study, three Gram-positive and three Gram-negative. Concerning with antimicrobial activity, results showed that plant extract of the studied species are a great source of phenolic compounds and represents the highest antibacterial activity against Gram-positive and negative bacteria. Results revealed that extracts represent the highest antibacterial activity against Gram-positive and negative bacteria and largest...
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Posted by admin on Oct 1, 2014 in |
A series of 1-(2-(4H-1,2,4-triazol-4-yl)ethylamino)-3-ch-loro-4-(5-methoxy/ehthoxy-2-substituted alkyl/aryl-1H-indol-3-yl)azeti-dinones (4a-4h) and 1-(2-(4H-1,2,4-triazol-4-yl) ethylamino)-3-chloro-4-(5-methoxy/ethoxy-2-substituted alkyl/aryl-1H-indol-3-yl) thiazolidi-nones (5a-5h) have been synthesized by the reaction of 3-((2-(2-(4H-1,2,4-triazol-4-yl)ethyl)hydrazono)methyl)-5-methoxy/ethoxy-2-substi-tuted-1H-indoles (3a-3h) with chloroacetyl chloride and thioglycolic acid respectively. All the newly synthesized compounds were screened for their antibacterial activity against S. aureus, E. coli, P. vulgaris, K. pneumonia and antifungal activity against A. fumigatus, C. albicans, C. albicans ATCC and C. krusei G03. The antibacterial activity of newly synthesized compounds compared with standard drug gatifloxacin and ciprofloxacin against different bacteria and antifungal activity of new compounds compared with standard drug fluconazole against different fungi. The compounds 5d, 5e, 5g, and 5h exhibited good antibacterial activity while compounds 4g, 5g, and 5h also showed notable antifungal activity. The purity of the newly synthesized compounds was checked by thin-layer chromatography (TLC) on silica gel-G coated plates using different solvent systems. The structure of all the compounds was established by the elemental (C, H, N) and spectral (IR, 1HNMR, and mass)...
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