Posted by admin on Nov 1, 2013 in |
Background: Skin wound infected by Staphylococcus aureus (S. aureus) is a common and painful lesion. Tetracycline is commonly used as the antibiotic of choice for its treatment. Aim: to determine the effectiveness of hen egg white lysozyme and dextran conjugated lysozyme in decreasing the bacterial count of experimentally induced skin wound infection in mice. Methods; Lysozyme was conjugated with dextran under mild Maillard reaction conditions. Lysozyme and conjugated lysozyme were tested in vitro for their effectiveness against S. aureus and E. coli. Mouse model of skin wound infection was prepared in 6 group of mices (n=5) by 1cm × 1cm paravertebral laceration, extending to fascia created by scalpel. Five minutes after wounding, 10 µl culture media suspension containing 106 CFU⁄cm2 S. aureus was inoculated. Lysozyme and dextran conjugated lysozyme were applied as a 400µg/ml eucerin ointment base in treatment groups. Tetracycline was applied topically in the third group of mice. Results: Both Lysozyme and conjugated lysozyme exhibited in vitro antibacterial effect against S. aureus at 400µg/ml, but only the...
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Posted by admin on Oct 1, 2013 in |
A highly resourceful, elegant, simple and green technique with exceptionally facile reaction conditions of one pot three component reaction was applied to synthesized series of novel mannich base having an array of biologically and pharmaceutically active novel heterocycles. The present protocol offered a valuable alternative to known methods and will find applications in the field of green synthesis and antimicrobial study against pathogenic microbes supports the development of bioinformatical database of novel and abandoned heterocycles. These data indicate their potential to become antimicrobial agents against pathogenic and resistant strains of micro-organisms. The novel products were established by Elemental, IR, mass spectroscopic and 1H, 13C NMR analysis. The environmental advantages of the method include short reaction time, excellent yield, easy work-up, elimination of extraction and chromatographic purification process. The synthesized β-amino-heteronapthols were tested for their antimicrobial efficacy against pathogenic bacteria and fungi by comparing their zone of inhibition with standard antibacterial agents (Gentamicin, Amoxicillin) and antifungal agents (Nistatin,...
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Posted by admin on Sep 1, 2013 in |
Antibacterial activity and protein content was studied in two species of octopus belonging to the family Octopoda. The highest zone of inhibition of about 34mm and 28mm were obtained against Vibrio parahaemolyticus and theprotein content was 107.16µg/L and 136.20µg/Lin Octopus dolfusii and Octopus aegina, respectively. SDS profile revealed bands of molecular weight of about 32.83 KDa to 72.36 KDa for both the species. Conclusively, the factors analyzed prove that the sample species play an important roles in chemical defensive mechanism against pathogens causing diseases in human and fishes. This study paves a way for further pharmaceutical research against pathogenic bacterial strains producing valuable...
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Posted by admin on Sep 1, 2013 in |
The purpose of the study is to evaluate the antimicrobial and cytotoxic activity of methanolic and pet-ether extract of leaves of Mesua ferrea. The antimicrobial activity was investigated against eight pathogenic Gram-positive and Gram-negative bacterial species at three concentrations (100, 300, 500 µg/disc) by using disc diffusion method. Both extracts showed moderate antibacterial activity against six bacterial species among eight and the activity was increased with increasing concentration of the sample. The maximum antimicrobial potential was obtained against Bacillus subtilis, Bacillus megaterium and Staphylococcus aureus in case of both extracts. The cytotoxic effect of the extracts was assayed on Artemica salina leaches. The LC50 values were found to be 7.94 μg/mL for methanolic extract and 3.98 μg/mL for pet-ether extract compared to 0.451 μg/mL for standard drug vincristine sulphate. The study confirms that the plant extracts have moderate antimicrobial activity and considerable amount of cytotoxic...
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Posted by admin on Sep 1, 2013 in |
Medicinal plants form the backbone of traditional system of medicine in India. Biotechnological and Pharmacological studies have acknowledged the value of medicinal plants as potential source of bioactive compounds. Phytochemicals from medicinal plants serve as lead compounds in drug discovery and design. Medicinal plants are rich source of novel drugs that forms the ingredients in traditional systems of medicine, modern medicines, nutraceuticals, food supplements, folk medicines, pharmaceutical intermediates, bioactive principles and lead compounds in synthetic drugs. Aloe is a genus containing about four hundred species of flowering succulent plants belonging to Liliaceae family. Aloe vera is as old as civilization and through history it has been used as a popular folk medicine. It is present in the arid regions of India. Aloe vera has valuable medicinal properties and is commercially used in pharmaceuticals, cosmetics and food industries. Since ancient time Aloe vera L. are using in many herbal preparations. The Aloe vera plant contains the important antioxidant components such as vitamins (A, C and E), B (thiamine), niacin, B2...
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