Posted by admin on Oct 31, 2023 in |
Raphanus sativus L, belongs to the family Brassicaceae. Phytochemical analysis of its methanolic root extract revealed the presence of alkaloids, flavonoids, glycosides, tannin, phenolic compounds, triterpenoids and steroids. The antimicrobial activities and in-vitro Pharmacological activities like Antioxidant Activity and Anti-inflammatory activity of the root extract was assessed. Production of reactive oxygen species (ROS) causes various diseases and cellular anomalies in human beings. Antioxidants inhibit generation of reactive species, or scavenge them, or raise the levels of endogenous antioxidant defenses. In this study the radical scavenging ability different extracts of Raphanus sativus L. was undertaken. Results revealed that the root of Raphanus sativus L. has potent antioxidant ability. A good correlation was found to exist between concentration of extract and % inhibition of protein denaturation and free radicle damage. From different literature reports, root extract was found to be more potent antioxidant than the other parts of the plant. The extract had shown significant anti-bacterial activity against bacterium like Lactobacillus, Streptococcus aureus, Escherichia coli against standard Tetracycline by the determination...
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Posted by admin on Oct 31, 2023 in |
The aim of this study was to evaluate the antimicrobial and antibiofilm activity of silver nanoparticles of Azadirachta indica (AISNPs) against the pathogens isolated from effluent. Green synthesis approach was opted for the synthesis of the synthesis of AISNPs by using the ethanolic extract of Azadirachta indica which was added to 1mM solution of AgNO3. These synthesized nanoparticles were molecularly characterized by UV-Visible spectrophotometer, TEM, Zeta Sizer, Zeta Potential, XRD and FTIR respectively. Antimicrobial activity of the AISNPs was carried by well diffusion method on Muller Hinton Agar against biofilm-producing multi-drug resistant effluent pathogens, which were identified as E. coli, P. aeruginosa, S. aureus and K. pneumoniae. The zone of inhibition of AISNPs observed in case of E. coli was 16.08 mm, 18.65 mm against S. aureus, 10.46 mm against P. aeruginosa and 15.34 mm against K. pneumoniae. Antibiofilm activity of AISNPs demonstrated the decline of biofilm producing activity by 76% against P. aeruginosa, 74% against S. aureus, 60% against K. pneumoniae and 59% against E. coli respectively, indicating...
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Posted by admin on Sep 30, 2023 in |
Antimicrobial proteins and peptides are also known as Host defense system. Recently, research in the food industry and therapeutic fields has seen a considerable expansion in the use of antimicrobial proteins. It has a wide spectrum of antibacterial and immunomodulatory properties against infectious bacteria, viruses, fungus, etc., obtained from microbes, plants, animals, and even humans. Antimicrobial peptides can potentially improve the specificity and efficacy of antibiotics by being a promising alternative because microorganisms can’t metabolize them. Regulation, post-transduction modification, ribosome synthesis, etc. are the essential components of the AMP (Antimicrobial protein and peptide) biosynthesis process. Today, AMPs can be developed synthetically based on their activity, source, structure, and species of amino acids. They have the unique ability to rupture the cell wall, which results in cell death. As a novel approach, scientists have determined a few strategies to administer AMPs by formulating with chitosan nanoparticles, gold and silver nanoparticles, and peptide-assembly by producing microgels, mesoporous materials, and other substances that can be applied in the fields of skin disease,...
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Posted by admin on Sep 30, 2023 in |
ZnO is used as a nanofiller in various concentrations to create flexible self-standing films of PVP-PVA mix composites. This work presents the results of structural, compositional, morphological, and optical analyses carried out using X-ray diffraction (XRD), Fourier Transform Infra-Red spectroscopy (FTIR), scanning electron microscopy (SEM), Thermo gravimetric analysis (TGA), and ultraviolet-visible spectroscopy (UV-vis). The XRD data show that ZnO nanoparticles are produced in the polymeric matrix with hexagonal phase. ZnO nanofiller is dispersed throughout the polymer matrix, as seen in SEM pictures. UV-vis spectra show that the nanocomposite films’ wavelength absorption peak is between 235 and 370 nm. The blue shift may be seen with a drop in the nano filler’s...
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Posted by admin on Aug 31, 2023 in |
Fatty acids produced by plants protect themselves from pathogens, such as multidrug-resistant bacteria. In the present work, crude extracts, and fatty acid fractions of four pteridophyte species from Assam, Northeast India, were evaluated for their antimicrobial properties, and the bioactive compounds were identified using GC-MS analysis. The crude extract of Pteris semipinnata (Ps), Lygodium microphyllum (Lm), Lycodium flexuosum (Lf), and Lycopodiella cernua (Lc) was found to possess antibacterial and antifungal activities. The lowest MIC and MBC values were 0.25 mg/ml and 0.5 mg/ml for the fatty acid fractions against most of the microbial test strains. The GCMS analysis revealed 21 compounds in Ps, 35 in Lm, 33 in Lc, and 52 in Lf fractions. The present work supports the use of fern species in traditional medicine and...
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