Posted by admin on May 31, 2023 in |
Objectives: The main intention of the current study was to isolate, antimicrobial assay and amylase screening of fungal strains SAPB-15 and SAPB-20 isolated from freshwater fish culturing ponds of Kaikaluru and Mudinepalli villages, South-East coast of Andhra Pradesh, India. Methods: The water samples were collected from different fish culturing aquatic ponds and transported to the laboratory. A serial dilution plate technique was employed for fungi isolation by using Czapek-Dox agar and Sabouraud dextrose agar media. Twenty fungal strains were isolated, all of them were screened for their antimicrobial and amylase production. The potential fungal strains were designated as SAPB-15 and SAPB-20. Partial characterization of the strains was carried out based on cultural, micro-morphological characteristics. Results: The potent fungal strains were provisionally identified as Penicillium species and Aspergillus species. The secondary metabolites produced by strains inhibited several test bacteria and fungi and the strains also exhibited a positive response to amylase production. Conclusion: Aquatic ponds are treasure houses for potential microbes. Among the twenty fungal strains, SAPB-15 and SAPB-20 were...
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Posted by admin on May 31, 2023 in |
Indeed, Actinomycetes and Penicillium are microorganisms that live in different ecosystems. Besides, they play important roles in various fields in respect such as agriculture, medicine and the environment. During this study, we were interested in the isolation of two strains namely: Actinomycetes and Penicillium sp. from coconuts and truffle respectively. In addition, the agar cylinder technique demonstrated the antibacterial activity of Penicillium sp. against four pathogenic bacterial strains (S. aureus, E. coli, P. aeruginosa and K. pneumoniae). In virtue of which, the results showed a variability of the diameters of the inhibition zones, thus the most important results were recorded against K. pneumoniae and S. aureus, which produced inhibition zones of 17.50±0.71 mm and 17.00±0.00 mm respectively. In order to struggle against pathogenic fungal strains, the ability of Actinomycete strain to suppress the mycelial growth of 03 pathogenic strains of Fusarium oxysporum f.sp. albedinis (EF1, EF2 and EF3) was examined. Hence, the result of the direct confrontation tests on the PDA medium between the Actinomycete strain and these pathogenic...
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Posted by admin on May 31, 2023 in |
An Antibiotic is an infectious disease that is the chief source for treating bacterial & fungal infections. The microbial resistance problem has been increasing daily, and the viewpoint for the usage of antimicrobial drugs is still undefined. Antimicrobial-resistant organisms are found in people, animals, food, plants, and in the environment. Do not use antibiotics to treat viral infections like colds and flu. The leading mechanism for spreading the resistance of antibiotics over bacterial populace is plasmids. Biofilm formation decreases the penetration of antifungal compounds into the population. According to World Health Organization (WHO) medicinal plants would be the best source to attain a variety of drugs. 80% of people from developed countries use traditional medicine derived from medicinal plants. Using plant extracts and phytochemicals with identified antimicrobial properties can be greatly useful in treatments. Several plants rich in a wide variety of secondary metabolites, such as tannins, alkaloids, and flavonoids, have been used for their antimicrobial qualities. They can spread from person to person or between people and animals,...
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Posted by admin on May 31, 2023 in |
Using plant extracts to synthesize metal nanoparticles is one of the easiest, most convenient, cost-effective, and ecologically acceptable ways of avoiding hazardous chemicals. As a result, many environmentally acceptable methods for the fast production of silver nanoparticles utilizing aqueous extracts of plant components such as leaves, bark, and roots have been described in recent years. This study highlights and expands on recent discoveries in the green production of silver nanoparticles (AgNPs) utilizing various plant extracts and their prospective uses as antimicrobial agents. A systematic in-depth discussion on the possible influence of phytochemicals and their concentrations in plant extracts, extraction solvent, and extraction temperature, as well as reaction temperature, pH, reaction time, and precursor concentration. Extensive details of the probable mechanism of AgNPs’ interaction with microbe cell walls, which leads to cell death and strong antibacterial activity, have also been developed. Furthermore, even though chemical techniques for shape-controlled synthesis are well-known, regulating the shape of biosynthesized AgNPs has numerous beneficial impacts on its...
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Posted by admin on Apr 30, 2023 in |
In recent days, the increasing number of microbes and their increasing resistance power against conventional drugs have led to enormous worldwide mortalities, hence they pose a great threat to human health. The modern era is already going through the threat of COVID-19, also caused by one of those microbes called the virus. In order to get a clear understanding, all the microbes have been classified in certain types. Nowadays, to develop new alternative antimicrobial medicines, scientists must acquire clarity about the responsible functional groups of different conventional drugs with proper mechanistic elucidation on different types of microbes. This information not only clarifies the functionalities and properties responsible for exhibiting antimicrobial effects, but also facilitates the idea of new drug development through proper functional group incorporation or modification. These modifications increase the efficacy of antimicrobial drugs as well as their activity and water solubility. In this review, my focus will majorly be on the four main types of microbes and their possible mechanistic elucidation of commonly used antibiotics and alternative...
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