Posted by admin on May 31, 2024 in |
The study was carried out to isolate and characterize pigment producing bacteria from rhizosphere soil. In our study, isolation and characterization of bacteria that produced pigments were performed using serial dilution, spread plating method followed by gram staining and various other biochemical tests (Oxidase, Catalase and IMViC tests). Our study concluded that bacteria isolated from rhizosphere soil are capable of producing pigment which is flexirubin (light orange in colour) and the bacterium belongs to the genus Chryseobacterium. After extracting the pigment, antimicrobial activity was performed, and it showed that the pigment has antimicrobial activity against both bacteria (E. coli and B. sphaericus) and fungi (Candida albicans). At 100µg/ml concentration, the pigment showed highest antioxidant activity with an absorbance of 0.116AU. The anti-inflammatory activity of the pigment was found to be highest at 1200µg/ml with an absorbance of 0.128AU. It is found that the pigment has the ability to increase SPF property of commercially available sunscreen by 0.713%. Thus different potentials of the pigment can be applicable in various industrial...
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Posted by admin on May 31, 2024 in |
Microbial chitosanase has received greater attention for producing chitooligosaccharides. In this study, chitooligosaccharide hydrolysate was developed using the chitosanase enzyme, and its bioactivity was evaluated. Marine muddy samples proved to be a source of isolating novel chitosan-degrading bacteria when compared to the other samples collected. A chitosan-degrading marine bacterium was isolated, and the characterization of its extracellular enzyme, chitosanase, was studied. The molecular characterization and 16S rDNA sequence evolutionary relatedness of the isolate were carried out. The organism was identified as Brevidomonas diminuta, and its sequence was deposited. The production of chitosanase enzyme was significantly induced by the chitosan substrate, while it reached its peak after 72 hours. pH and temperature have been verified to be ideal for enzyme synthesis at 6.5 and 30 °C, respectively. Utilizing a culture medium containing xylose as the substrate at a level ranging from 1.0 to 1.5% significantly enhanced the enzyme production. The molecular weight of the enzyme, 43 KDa, was ascertained through SDS-PAGE. The pathogen’s growth was suppressed by the chitooligosaccharide hydrolysate...
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Posted by admin on May 31, 2024 in |
Phytosterols, a subclass of steroids, have gained considerable attention in scientific research and nutrition due to their potential health benefits. This article presents an overview of phytosterols, encompassing their sources, chemistry, extraction methods, analytical techniques, and biological activities. Phytosterols, structurally resembling cholesterol, are found abundantly in plants, animals, and fungi. Their physiological roles include influencing membrane fluidity and acting as second messengers in signal transduction pathways. Incorporating phytosterol-rich foods into the diet has been linked to various health benefits, particularly in reducing cholesterol levels, as recognized by regulatory bodies like the European Food Safety Authority and the FDA. The article discusses various extraction methods for phytosterols, emphasizing the need for environmentally friendly approaches to minimize the use of organic solvents. Traditional techniques such as Soxhlet extraction are effective but pose health and environmental concerns, driving the exploration of alternative extraction technologies. Phytosterol chemistry, characterized by specific carbon atoms and side chains, is explored, highlighting their steroid structure and different forms in nature. Analytical methods such as gas chromatography (GC),...
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Posted by admin on Apr 30, 2024 in |
Punica granatum L. is the reddish colour fruit, commonly known as Pomegranate. It is known to have many health and therapeutic benefits which can be used as agent. The pomegranate juice is also used to prepare wine. The present study was carried out to determine the optimum fermentation parameters of pomegranate wine preparation. The antimicrobial and antioxidant activity of various wine preparations were also evaluated for their health benefits. The wines were prepared using various brix values under both aerobic and anaerobic conditions. The antimicrobial and antioxidant activity which was carried out using the agar cup method and DPPH assay respectively. The best wine was found to have 15.3% alcohol prepared with 0°B and aerobic conditions in 8 days. The antimicrobial and antioxidant activity of the wines prepared were higher than the fresh P. granatum juice. Therefore, we suggest that pomegranate wine is a better option of fruit juice storage having better antimicrobial and antioxidant...
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Posted by admin on Mar 31, 2024 in |
Thespesia populnea Linn., a member of the Malvaceae family also known as Portia tree, a plant commonly found in coastal and tropical regions of India. Its therapeutic properties have been recognized in ayurvedic medicine, which has been validated by modern scientific research. This paper highlights the chemical constituents and pharmacological profile of Thespesia populnea. The plant has shown remarkable potential as an anti-inflammatory, antidiabetic, antimicrobial, antipsoriatic, antidiarrheal, anti-implantation, anticancer, and alzheimer’s disease management agent etc. Its extracts also exhibit a synergistic activity with oxytetracycline, a commonly used antibiotic. Additionally, the plant is used as a natural dye for various fabrics, a source of biodiesel and an anti-corrosive agent. Phytochemical analysis has exposed the presence of alkaloids, sesquiterpenoids, fatty acids, polysterol, glycosides and alkane in various extracts of the plant. These bioactive compounds are responsible for the diverse pharmacological activities exhibited by Thespesia populnea. The review provides advanced evidence to support the traditional knowledge of Thespesia populnea and highlights its potential as a source of novel drugs for various diseases....
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