Posted by admin on Jun 30, 2024 in |
Food safety is at risk from several variables, including physical, chemical, and biological risks. In this context, biological risks are of utmost significance. The bacteria among them are crucial in producing food deterioration and food-borne illnesses. Additionally, a novel strategy has been applied in recent years that relies on pro-biotic and post-biotic to inhibit the increase of pathogenic microbes and the mediated corruption they cause because of their potent antimicrobial characteristics. The word “postbiotic” designates soluble elements produced by live bacteria or released following bacterial lysis, including enzymes, peptides, teichoic acids, muropeptides produced by peptidoglycans, polysaccharides, cell surface proteins, and organic acids. Postbiotics have a significant potential to be employed in the pharmaceutical, food, and nutraceutical sectors to improve health and disease prevention due to a variety of signaling substances that may have antioxidant, immune-modulatory, anti-inflammatory, antihypertensive, and antiproliferative effects. The current investigation was to determine the antagonistic potential of the secreted probiotic filtrates, also known as postbiotics, generated from some microorganisms against pathogenic microbes such as Candida albicans,...
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Posted by admin on May 31, 2024 in |
The study was carried out to isolate and characterize pigment producing bacteria from rhizosphere soil. In our study, isolation and characterization of bacteria that produced pigments were performed using serial dilution, spread plating method followed by gram staining and various other biochemical tests (Oxidase, Catalase and IMViC tests). Our study concluded that bacteria isolated from rhizosphere soil are capable of producing pigment which is flexirubin (light orange in colour) and the bacterium belongs to the genus Chryseobacterium. After extracting the pigment, antimicrobial activity was performed, and it showed that the pigment has antimicrobial activity against both bacteria (E. coli and B. sphaericus) and fungi (Candida albicans). At 100µg/ml concentration, the pigment showed highest antioxidant activity with an absorbance of 0.116AU. The anti-inflammatory activity of the pigment was found to be highest at 1200µg/ml with an absorbance of 0.128AU. It is found that the pigment has the ability to increase SPF property of commercially available sunscreen by 0.713%. Thus different potentials of the pigment can be applicable in various industrial...
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Posted by admin on May 31, 2024 in |
Microbial chitosanase has received greater attention for producing chitooligosaccharides. In this study, chitooligosaccharide hydrolysate was developed using the chitosanase enzyme, and its bioactivity was evaluated. Marine muddy samples proved to be a source of isolating novel chitosan-degrading bacteria when compared to the other samples collected. A chitosan-degrading marine bacterium was isolated, and the characterization of its extracellular enzyme, chitosanase, was studied. The molecular characterization and 16S rDNA sequence evolutionary relatedness of the isolate were carried out. The organism was identified as Brevidomonas diminuta, and its sequence was deposited. The production of chitosanase enzyme was significantly induced by the chitosan substrate, while it reached its peak after 72 hours. pH and temperature have been verified to be ideal for enzyme synthesis at 6.5 and 30 °C, respectively. Utilizing a culture medium containing xylose as the substrate at a level ranging from 1.0 to 1.5% significantly enhanced the enzyme production. The molecular weight of the enzyme, 43 KDa, was ascertained through SDS-PAGE. The pathogen’s growth was suppressed by the chitooligosaccharide hydrolysate...
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Posted by admin on May 31, 2024 in |
Phytosterols, a subclass of steroids, have gained considerable attention in scientific research and nutrition due to their potential health benefits. This article presents an overview of phytosterols, encompassing their sources, chemistry, extraction methods, analytical techniques, and biological activities. Phytosterols, structurally resembling cholesterol, are found abundantly in plants, animals, and fungi. Their physiological roles include influencing membrane fluidity and acting as second messengers in signal transduction pathways. Incorporating phytosterol-rich foods into the diet has been linked to various health benefits, particularly in reducing cholesterol levels, as recognized by regulatory bodies like the European Food Safety Authority and the FDA. The article discusses various extraction methods for phytosterols, emphasizing the need for environmentally friendly approaches to minimize the use of organic solvents. Traditional techniques such as Soxhlet extraction are effective but pose health and environmental concerns, driving the exploration of alternative extraction technologies. Phytosterol chemistry, characterized by specific carbon atoms and side chains, is explored, highlighting their steroid structure and different forms in nature. Analytical methods such as gas chromatography (GC),...
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Posted by admin on Apr 30, 2024 in |
Punica granatum L. is the reddish colour fruit, commonly known as Pomegranate. It is known to have many health and therapeutic benefits which can be used as agent. The pomegranate juice is also used to prepare wine. The present study was carried out to determine the optimum fermentation parameters of pomegranate wine preparation. The antimicrobial and antioxidant activity of various wine preparations were also evaluated for their health benefits. The wines were prepared using various brix values under both aerobic and anaerobic conditions. The antimicrobial and antioxidant activity which was carried out using the agar cup method and DPPH assay respectively. The best wine was found to have 15.3% alcohol prepared with 0°B and aerobic conditions in 8 days. The antimicrobial and antioxidant activity of the wines prepared were higher than the fresh P. granatum juice. Therefore, we suggest that pomegranate wine is a better option of fruit juice storage having better antimicrobial and antioxidant...
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