Posted by admin on Dec 1, 2014 in |
The objective of the present study is to develop a rapid HPTLC method for the identification, separation and quantification of some amino acidsfrom two species of Marchantia. Three different mobile phases were used for the separation and estimation of eight amino acids (alanine, glycine, methionine, threonine, valine, leucine, histidine and isoleucine). TheHPTLC analysis of Marchantia palmata and M. nepalensis, showed the presence of all the eight amino acids. The content of all the amino acids in Marchantia nepalensis were higher than M. palmata. Of all the amino acids, the glycine content (0.417 mg/g dw in Marchantia nepalensis and 0.287 mg/g dw in M. palmata) was found to be maximum and valine content (0.053 mg/g dw in Marchantia nepalensis and 0.031 mg/g dw in M. palmata) was found minimum. The presence and content of amino acids in the liverworts has been for the first time evaluated by a modern technique like HPTLC. This method provides an important tool for the quantitative evaluation of important amino acidsandgives more accurate results than...
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Posted by admin on Dec 1, 2014 in |
Ravenala madagascariensis Sonn. is one of the most popular medicinal plants in Madagascar, India and several African countries. It is used in the treatment of many diseases, and particularly in those of diabetes. The duration of treatment, often for life, and its relatively high cost for certain social categories, constrain more and more patients to use this plant. Its chemical constituents were still little studied and this problem could create significant damage in case of intoleranceor abuse during its use.The present study undertaken on the leaves of Ravenala madagascariensis, led to the isolation and structural elucidation of two new compounds (1 and 2), together with the known cycloartanol triterpene (3) which was isolated for the first time from this plant. Their structures were elucidated as (2E, 7R, 11R) phytyl-3, 7, 11, 15-tetramethylhexadec-2-enyl pentadecanoate (1) and (24S, 31S)-cycloartan-31, 32-diol (2). Structural determinations were made on the basis of analysis of 1D and 2D-NMR spectroscopic data. The complete 1H and 13C resonance assignments were also carried out by using 1H-1H COSY,...
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Posted by admin on Sep 1, 2014 in |
Aim: H1 Antihistamines are classified into the first generation and second generation agents. The main differences between the first and second generations of drugs are their propensity to cause central nervous system (CNS) side effects. Therefore, the present study was aimed to analyze the effects of different H1 antihistamines (first and second generation) on CNS using different animal experimental models. Materials and Methods: H1 antihistamines such as pheniramine maleate (3 mg/kg, 6 mg/kg), cetirizine (0.6 mg/kg, 1.2 mg/kg), levocetirizine (0.6 mg/kg, 1.2 mg/kg), loratadine (1 mg/kg, 2 mg/kg) and desloratadine (0.6 mg/kg, 1.2 mg/kg) are evaluated and compared for their effects on CNS using experimental animal model (Pentobarbitone sleeping time, spontaneous motor activity, motor coordination) in Swiss albino mice. Results and Discussion: Desloratadine (0.6 mg/kg, 1.2 mg/kg) and loratadine (1 mg/kg, 2 mg/kg) did not produce significant (P<0.05) effect on sleeping time when compared to control. At 120 min time interval after treatment with cetirizine (1.2 mg/kg) and levocetirizine (1.2 mg/kg) was shown a reduction in locomotor activity and...
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Posted by admin on Feb 1, 2014 in |
The objective of the current study was to develop and optimize sublingual tablets of Terazosin Hydrochloride, which is an effective drug in the treatment of Benign Prostate Hyperplasia, Hypertension. Sublingual tablets of Terazosin Hydrochloride were prepared by direct compression method using different superdisintegrating agents such as Crosspovidone, Sodium starch glycolate and Crosscarmellose sodium. The tablets were evaluated for pre-compression studies like Bulk density, Tapped density, Carr’s index, Hausner’s ratio and post-compression studies like Thickness, Hardness, Weight variation, Friability, drug content, Wetting time, Water absorption ratio, in-vitro disintegration time, in-vitro dispersion time, in-vitro dissolution study and also drug release kinetic study. The Hardness, Weight variation, Thickness, Friability and Drug content of tablets were found to be acceptable according to pharmacopoeial limits. An optimized formulation i.e. F6 was found, which provided short wetting time of 67sec, water absorption ratio of 39.01 , in-vitro disintegration time of 61sec and in-vitro dispersion time of 112sec. From the above results It indicated that the amount of superdisintegrant i.e. Crosspovidone was significantly affected the dependent...
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Posted by admin on Jan 1, 2014 in |
The aim of the present study was to investigate the oil composition extracted from Sesamum indicum seeds. The oil was extracted from seeds by soxhlet method and analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 84 different components were identified and out of which 12 are fatty acid components. The principal compounds identified and present in all samples were silane, trimethyl[5-methyl-2-(1-me thylethyl)phenoxy]-; silicic acid, diethyl bis (trimethylsilyl)ester; stearic acid; oleic acid; n-Hexadecanoic acid; myristic acid; indole-2-one 2,3-dihydro-N-hydroxy-4-methoxy-3,3-dimethyl-; cyclotrisiloxane, hexamethyl-; acetamide, N-[-4-(trimethylsilyl)phenyl]-; benzene, 2-[(tert-butyldimethylsilyl)oxy]-1-isopropyl-4-methyl-; benzo[h]quinoline, 2,4-dimethyl-, trichloroacetic acid undec-2-enyl ester and 2-Ethylacridine. Among fatty acids, palmitic acid and oleic acid were identified in highest concentration. In comparison, myristic acid and stearic acid were present in lesser amount. Variation was found between chemical compositions of different genotypes of sesame. The obtained results supported the use of seed oil as edible oil and in pharmaceuticals. This study is also important as identification of volatile compounds in Indian genotypes of Sesamum indicum was done for the first...
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