Posted by admin on Mar 31, 2021 in |
Jimson weed (Datura stramonium) members of the Solanaceae plant family; serve as major folklore medicinal herbs. The biologically active compounds spectrum in leaves of Datura stramonium L. was investigated by GC-MS with NMR & FTIR analysis. Steroids, terpenoids, anticholinergic alkaloids, atropine, and scopolamine, are detected. Twenty of them are new constitutions for the species, and eight phytosterol compounds are described for the first time. This is the first report on some other antibacterial heterocyclic compounds, antioxidant flavonoids, antimicrobial phytol, amino acids, and sulfone & fluoro compounds in Datura stramonium leave identified by GC-MS...
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Posted by admin on Aug 31, 2019 in |
Proteins interact in the cellular arena to carry out a majority of the vital functions of the cells. After exploring the transcriptome, the researchers are now targeting to investigate the interactome of a cell. This can help to design more efficient drug and vaccine formulations and therapeutic targets benefiting humankind in every possible way. Advancement in protein and protein networking databases gives the first insight to study such macromolecular interactions paving the way for their in-vitro and in-vivo analysis. However, none of the technique can promise 100% efficiency alone due to some limitations like time, cost, and false positive results. Combination of one or more techniques can enhance data quality and reliability. Recent advancements in the studies of the protein-protein interaction using computational methods have led the identification of different signaling pathways and protein complex involved in various specific diseases. However, the major challenge faced is to verify the results obtained from the virtual prediction software’s. In this review, we have focused on how different techniques have been accustomed...
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Posted by admin on Jul 31, 2019 in |
One of the major problems faced by the pharmaceutical industry is to maintain the quality and efficacy of herbal drugs due to the lack of purity of herbal raw materials. Furthermore, using traditional identification methods to identify the correct plant part needed for the preparation of herbal medicine can be difficult. Creating a novel system to assess the quality of a medicinal herb and to discriminate adulterants from authentic raw material is essential. There is a lot of confusion regarding nomenclature and originality of Valerian available in the market. The objective of the present work is to develop a molecular tool for accurate identification of dry root samples. The isolation of DNA is the first step in developing this technology. The methods employed for extracting DNA from fresh and dried root samples of medicinal plants, however, are time-consuming and yield DNA in lesser quantity and low purity due to the presence of high levels of polysaccharides, phenolics and other secondary metabolites in these samples. In this study, an easy...
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Posted by admin on Feb 28, 2019 in |
The present work encompasses the development of two classical UV spectroscopy methods and an RP-HPLC method for the concurrent assessment of Halobetasol propionate and Mupirocin inculcating a statistical approach. The UV spectroscopy methods developed herein include absorption ratio method and first derivative spectroscopy method. The wavelength maxima selected for Absorption ratio method were 240 and 220 nm for Halobetasol Propionate and Mupirocin respectively. The zero crossing points selected were 240.626 nm and 220.180 nm for Halobetasol Propionate and Mupirocin respectively for first derivative spectroscopy. The chromatographic separation was achieved by using CHROMBUDGET C18 (250 × 4.6 mm) 5 µm column, mobile phase consisting of Acetonitrile: phosphate buffer (65:35 v/v, pH 3.2), at 1 ml/min flow rate and detection wavelength was 230 nm The retention time of Halobetasol propionate, and Mupirocin was found to be 8.647 ± 0.06 min and 3.357±0.123 min, respectively. Linearity was observed in the concentration range of 5.125 – 5.75 µg/ml for Halobetasol propionate and 5-30 µg/ml for Mupirocin respectively. The analytical method was validated as...
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Posted by admin on Mar 1, 2016 in |
Pressure ulcer (PU) is a complex and multifactor disease in which the cellular and molecular mechanisms contributing to risk of a delay in healing. An imbalance between pro-oxidant and antioxidant systems has been suggested to be implicated in the physiopathology of PU. Polymorphisms in TNF-α gene are emerging as key determinants of many diseases. The TNF-α (-238 and -308) G/A single-nucleotide polymorphisms (SNPs) are the most extensively studied. This study aimed to assess the value of serum pro-oxidant and antioxidant levels and to determine the role of a TNF-α gene SNPs in the pathogenesis of PU. 100 Tunisian subjects suffering of PU and 213 controls were admitted. Oxidant status was evaluated by the measure of homocysteine and thiobarbituric reactive oxygen substances. Antioxidant status was evaluated by the measure of total antioxidant status, serum catalase activity and trace elements. G308A and G238A variants of TNF-α gene were screened by AS-PCR and RFLP-PCR. Our results suggest that the unbalance between pro-oxidants and antioxidants seems to be more aggravated in patients suffering...
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