Posted by admin on Nov 30, 2020 in |
Electrophoresis is an effective analytical tool for separation, as it does not affect the molecular structure, and it is also highly sensitive to small differences in molecular charge and size of the analytes. Capillary electro-phoresis is a separation technique where the ions are separated based on their electrophoretic mobility with an applied voltage. So far, the research field of capillary electrophoresis remains very active, as exhibited by a steady and significant flow of scientific reports on theory, separation modes, new instrumentation, and applications of CE techniques in various areas. Capillary electrophoresis is the most predominately used because it gives faster results and it provides a high-resolution separation. It is one of the useful techniques because there is a large range of detection methods available. This paper is devoted to providing a brief explanation of types of electrophoresis; capillary electrophoresis, and CE-based separation modes with some advantages and disadvantages along with applications. This paper also includes some of the hyphenation techniques based on capillary electrophoresis (CE-MS, CE-ICP-MS, CE-ESI-MS, CE-MALDI-MS, and...
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Posted by admin on Nov 30, 2020 in |
Objective: Arjunarishta is an important Ayurvedic formulation with chief ingredient Arjuna (Terminalia arjuna), which is excellent “Hrudya” Dravya. It nourishes & strengthens heart muscles & promotes cardiac functioning by regulating blood pressure and cholesterol level. This study aims to established quality parameters for standardization of Ayurvedic formulation “Arjunarishta” on the basis of various techniques viz. Physico-chemical screening, Chromatography & IR Spectroscopy. Method: Conventional analysis such as Organoleptic tests, pH, Alcohol content, Brix, and Specific gravity was done. Chromatographic analysis was performed to estimate Arjungenin, Ellagic acid, and Gallic acid using High-Performance Thin Layer Chromatography (HPTLC). IR fingerprint was done using Fourier transform infrared (FT-IR) spectrometer. Result: The above analysis showed the pH of tested formulation ranges between 3.0-5.0, Brix 25-30%, and Alcohol content 6-10% v/v. Organoleptic tests confirmed characteristic odor of self-generated alcohol with sweet, astringent & slightly bitter taste. HPTLC analysis confirmed the presence of Arjungenin, Ellagic acid, and Gallic acid. FT-IR reveals unique transmittance spectra in the range of 4000 – 600 cm-1. Conclusion: This study can...
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Posted by admin on Nov 30, 2020 in |
The extracts of Boswellia serrata possess notable medicinal uses. Boswellia serrata is a highly lipophilic drug. It undergoes extensive hepatic first-pass metabolism. The t1/2 is approximately 6 h. There are reported side effects with the administration of Boswellia serrata. This research work aims to fabricate Elastosomes which get hold of many desired features for transdermal drug delivery. The Elastosomes are prepared using thin-film hydration technique using edge activators like Span 80, Tween 80, and Brij 30. The prepared elastosomes are evaluated for entrapment efficiency percentage (EE %), particle size (PS), polydispersity index (PDI), zeta potential (ZP), deformability index (DI), drug release study, and permeation study. EF-10 was regarded as the optimized formulation with all the desired characteristics. The optimized formulation (EF-10) resulted in better prolong drug release and sustained therapeutic action as compared to the marketed...
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Posted by admin on Nov 30, 2020 in |
Teff millet is progressively gaining importance worldwide due to its recognition as gluten-free food. Interestingly, its attractive nutritional profile and additional functions related to maintaining health play an important role in its identification as a nutraceutical food. The present study was conducted to analyze the nutritional, phytochemical, and antioxidant properties of teff millet. Moisture, ash, fat, fiber, protein, and carbohydrate were analyzed in this study. Minerals like calcium, iron, phosphorus, and zinc were also estimated. Result on proximate analysis showed moisture (10.5 g / 100 g), ash (2.2 g / 100 g), fat (0.5g/ 100 g), fibre (2.8 g / 100 g), protein (10.9 g / 100 g) and carbohydrate (73.1 g / 100 g) respectively. Result on mineral composition showed Calcium (168.1 mg /100 g), iron (13.1 mg / 100 g), phosphorus (420.2 mg / 100 g) and zinc (4.5 mg / 100 g) were also found in teff millet. The presence of different macro as well as micronutrients was recorded in teff millet. Phytochemical analysis indicated the...
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Posted by admin on Nov 30, 2020 in |
Increasing severity of the disease osteoporosis in postmenopausal women with long term exposure to pharmacotherapy has spurred interest in the development of using food as medicine and phyto-pharmacotherapy. We examined the effect of n-hexane crude leaves extract and its nanoparticle formulation of Sesbania grandiflora in cell proliferation and bone mineralization at different concentrations in Saos-2 cell line. Osteogenic differentiation and cell viability of Saos-2 cells were quantified by monitoring alkaline phosphatase activity and MTT assay. HPLC analysis confirms the presence of bioactive compound α tocopherol. It was found that the concentrations 10 µg & 20 µg/100 µL significantly induced cell proliferation (p<0.05), and the expression of ALP (p<0.01) was not inhibited by the presence of the bioactive compound. Our data suggest that S. grandiflora nanoparticles affect the Saos-2 cell health in higher dose concentration and will be a good natural herbal medicine candidate for the treatment of osteoporosis at lower dose concentration. Further research is required to gain insight into the plant bioactive compounds and the molecular mechanism involved...
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