Posted by admin on Apr 30, 2020 in |
The endeavor of the present work is to develop a simple, economical, efficient, novel green analytical method for the estimation of Amlodipine besylate, Olmesartan medoxomil and Hydrochlorothiazide in pharmaceutical formulation. Quantification was carried out using an Inertsil CN-3.5 μm (4.6 ×250 mm) column, where the mobile phase consisting of 10 mm Phosphate buffer (pH 3.0) and Acetonitrile (40:60). The flow rate was 1.0 mL/min and the effluent was monitored at 262 nm. The observed linearity was in the range of 5-25 µg/ml for Amlodipine (AMLO), Hydrochlorothiazide (HCTZ) and Olmesartan medoxomil (OLME) with a correlation coefficient of 0.997, 0.999 and 0.999 respectively. The proposed method was validated as per ICH guidelines in terms of linearity, accuracy, precision, robustness, and specificity, the limit of detection and limit of quantification. The method has been applied to Amlodipine, Hydrochlorothiazide and Olmesartan formulation without the interference of excipients of the...
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Posted by admin on Apr 30, 2020 in |
The aim of the current investigation was to develop bilayered immediate-release tablets of Telmisartan (TEL) and Hydrochlorothiazide (HCTZ) for the treatment of hypertension. In contrast to monotherapy, the dual drug therapy of TEL (an angiotensin II receptor blocker) and HCTZ (diuretic) is connoted to have a cumulative antihypertensive effect. Additionally, it offers ameliorative patient adherence to fixed-dose combination therapy over monotherapy and diminishes pill burden and dose-related side effects. The preformulation studies were accomplished by determining the compatibility of model drugs with their respective excipients by FTIR studies. These studies unambiguously connoted nix chemical interaction of excipients with the chosen model drugs. The formulation development was achieved in phases comprising of preliminary screening, pre-optimization and optimization studies. The wet granulation technique was adopted for formulating bilayer tablets. For pre-optimization studies, five batches for each layer (T1-T5 for TEL and H1-H5 for HCTZ layer) were prepared. Based on the outcomes of pre-optimization, the formulation batches T2 and H5 were subsequently chosen for optimizing the varied process and formulation variables. The...
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Posted by admin on Apr 30, 2020 in |
The mammalian central nervous system is composed of the brain and spinal cord. The brain is protected by the blood-brain barrier (BBB) that allows selective traffic of substances into the brain. Fluoride is a highly toxic and reactive element. We get exposed to fluoride due to its common use in toothpaste, dental gels, non-stick pans, razor blades, etc. Fluoride also enters our food chain through fishes that are cultivated in water bodies, which get contaminated with fluoride from running water from farms around it. Fluoride can accumulate on our body and cause toxic effects. In the context of fluoride, it is reported that fluoride exposure causes various toxic effects in our body, including the central nervous system as well. Fluoride is known to cross the blood-brain barrier and enter our brain tissue. It interferes with the normal metabolic process of the brain, generates free radicals and causes various toxic effects therein. Fluoride has been found to be linked to various pathogenic conditions of the brain as well. In this...
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Posted by admin on Apr 30, 2020 in |
Two simple spectrophotometric methods have been developed for the simultaneous estimation of Empagliflozin and Metformin hydrochloride from the tablet dosage form. Method-I simultaneous equation method involves the measurement of absorbances at two wavelengths 224 nm (λmax of Empagliflozin) and 233nm (λmax of Metformin hydrochloride) using Methanol and Water as diluent. Method – II Absorption ratio method involves the measurement of absorbances at two wavelengths 233 nm (λmax of Metformin Hydrochloride) and 266 nm (λmax of Isobestic point). The linearity lies between 0.1-25 µg/ml for Empagliflozin and 0.5-25 µg/ml Metformin hydrochloride in both methods. The accuracy and precision of the methods were determined and validated statistically. The two methods exhibited good reproducibility and recovery with a relative standard deviation of <2%. Both the methods were found to be rapid, specific, precise, accurate, and reproducible and can be successfully applied for the routine analysis of Empagliflozin and Metformin hydrochloride in a combined dosage...
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Posted by admin on Apr 30, 2020 in |
The present study deals with the phytochemical study of leaves of Xylosma longifolia Clos. It belongs to the family Flacourtiaceae and is commonly known as ‘Long leaved xylosma’, whereas the plant is locally known as ‘Kataponial’ in Assam. Xylosma longifolia is an important ethnomedicinal plant used in the North-eastern region of India. The aim of the study is to investigate the bioactive compounds present and to evaluate the significance of the therapeutic and pharmacological uses of the phytoconstituents. All the standard phytochemical and spectroscopic procedures were followed for the detection and estimation of the phytoconstituents. The leaves of Xylosma longifolia were collected for the preparation of 95% methanol extract. Preliminary phytochemical screening of the methanol extract of Xylosma longifolia reveals the occurrence of several secondary metabolites like alkaloids, flavonoids, phenols, tannins, terpenoids and saponins. The quantitative phytochemical analysis exhibited the presence of alkaloids, flavonoids and phenols in considerable quantity. The presence of O-H stretch, C-O stretch, C-H stretch, C-H bend, C=C stretch, N-H stretch and C=O stretch were confirmed...
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