Posted by admin on Jan 31, 2020 in |
Neuropathic pain is commonly comorbid with mood disorders, such as depression and anxiety. A state of depression may suggest biochemical imbalance that can increase the oxidative stress into the brain. The present study aimed to investigate the effect of aqueous extract of the red algae Sphaerococcus coronopifolius on depression-induced behavioral alterations and oxidative stress in female Wistar rats. Sciatic nerve injury was employed to induce depression in rats. Antidepressant-like effect of S. coronopifolius was investigated using two models: the forced swimming test (FST) and the open field test (OP). Three groups of rats were considered: The first group served as a control. The second group was subjected to sciatic nerve ligation (untreated group). The third group was subjected to sciatic nerve ligation treated daily by intraperitoneal injection of 25 mg algae extract kg−1 body weight of rat during two consecutive weeks(treated group). At the end of the experiment, rats were sacrificed and brain tissues were isolated to determine the activities of catalase (CAT) and glutathione-S-transferase (GST) as well as...
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Posted by admin on Jan 31, 2020 in |
The present study was aimed to assess the hepatoprotective activity of ethanolic extract of Coleus vettiveroides on experimentally induced hepatotoxic models. Phytochemical screening of EECV was carried out and the results showed the presence of carbohydrates, alkaloids, tannin, flavonoids, phenolics, proteins and aminoacid, steroids and triterpenoids. HPTLC screening of EECV was carried out along with different marker compounds to ensure the presence of phytoconstituents such as Quercetin, Rutin, and Gallic acid. Antioxidant activities of the herbal extract were determined using both DPPH and ABTS scavenging method. MTT assay is most widely used to determine the cytotoxic potential of medicinal agents and other toxic materials. It was observed that for different concentrations of EECV, the cell viability was greater than 90%. In-vitro hepatoprotective activity of EECV was evaluated on Hep G2 cells, the human hepatoma cell line. In Hep G2 treatment with EECV (12.5, 25, 50, 100 and 200 μg/ml) the cell viability was above 90% indicating that EECV showed no cytotoxicity up to 200 μg/ml. Evaluation of biochemical parameters...
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Posted by admin on Jan 31, 2020 in |
A plethora of herbs have been tested for their activity against ulcerative colitis, a debilitating disease in the group of IBDs. However, a promising adjuvant to conventional therapy remains still at large. Ellagic acid, a polyphenol widely available in berries have shown efficacy against ulcerative colitis in preclinical models. Owing to its high content of ellagic acid and its analogs, the stem bark of Terminalia tomentosa was chosen for our study. Effect of Terminalia tomentosa hydroalcoholic extract and its ellagic acid-rich fraction were tested for their anti-colitic potential in DSS (5%) induced ulcerative colitis in mice. The evaluation was carried out for assessing the severity of disease at macroscopic biochemical and histopathological level. Myeloperoxidase and superoxide anions, an inflammatory and an oxidative marker respectively were also evaluated in all the groups. Amongst all, the highest significance was observed in the group treated with an ellagic acid-rich fraction (200 mg/kg). The ameliorative effect has been attributed to the high antioxidant potential of the...
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Posted by admin on Jan 31, 2020 in |
A stability-indicating UHPLC method was developed for the simultaneous estimation of Sofosbuvir (SOF) and Ledipasvir (LPS) in bulk and tablet dosage form. The study was performed using C18 BEH column (210 mm × 50 mm, 1.7 µm), with a mobile phase consisting of acetonitrile (55% v/v) and phosphate buffer of pH 3 (45% v/v) at a flow rate of 0.3 ml/min. The detection was carried at 247 nm using PDA detector and the retention times were found to be 0.7 and 1.2 min for SOF and LPS respectively. The developed method was validated according to ICH guidelines and the results were statistically validated. The method was found to be linear in the concentration range of 80 – 240 µg/ml for SOF with r2 value 0.999 and 18 – 54 µg/ml for LPS with r2 value 0.999. The % RSD value for precision, measured as repeatability and intermediate precision was less than 2 for both the analytes. The recovery percentages were found to be 100.13 and 99.93 for SOF and...
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Posted by admin on Jan 31, 2020 in |
Acne vulgaris is a commonly diagnosed inflammatory skin condition that affects pediatric, young and adult patients. The object of the paper is formulation development and evaluation of isotretinoin gel for the treatment of Acne vulgaris. The isotretinoin based anti-acne gel was formulated using different polymers includes carbopol 940, carbopol 934 and carbopol 980. Disodium edetate is used as chelating agents. The anti-acne gel was evaluated for solubility, drug release, pH and drug interaction, in-vitro drug release through Franz diffusion cells, acute skin irritation test and antibacterial test. The evaluation test was also compared with the marketed formulation of isotretinoin gel, that is, sortet gel. The antibacterial and anti-acne activity of different formulations was determined by the modified agar well diffusion method in the culture of Propionibacterium acne. The optimized formulation (F-IB) showed the highest spreadability (48.623 g/cm3), in all formulations and also have a high percentage of drug contents (97.2%). In–vitro diffusion study suggested that F-IA, F-IC, F-IIA and F-IIB showed more diffusion, and drug release from all the...
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