Posted by admin on Dec 31, 2019 in |
Chromolaena odorata was extracted by ethanol/water. Centella asiatica was extracted by methanol. A cream containing C. odorata and C. asiatica extracts was formulated and evaluated for its physicochemical properties and stability. C. odorata and C. asiatica were extracted and their respective active compounds scutellarein tetramethyl ether and asiaticoside were determined as bioactive compounds using HPLC. Creams were prepared using 5% C. odorata and 1% C. asiatica extracts and tested for their appearance, pH, and viscosity. Stability was evaluated under accelerated conditions using six hot/cold cycles and after long-term storage at 30 °C/75% RH for 3 months. The content of scutellarein tetramethyl ether in C. odorata extract was 0.13 ± 0.02% w/w and the content of asiaticoside in the C. asiatica extract was 0.35 ± 0.04% w/w. Contents of scutellarein tetramethyl ether and asiaticoside in the cream formulation were 98.25 ± 0.43% and 102.16 ± 0.22%, respectively. The cream formulations containing the extracts had a good appearance and were non-greasy and easily applied, with a pH value of 5.4 ±...
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Posted by admin on Dec 31, 2019 in |
Cryptolepis buchanani Roem. & Schult. (Family: Asclepiadaceae) commonly-known as Wax leaved climber, Kali Sariva in English and Sanskrit. It is broadly used in Ayurveda as a remedy of ailment. Though, its diverse medicinal aspects, no detail pharmacognostical study is available till date. The present study is to investigate the pharmacognostical characteristics of C. buchanani aerial part. The investigation was carried out in terms of macroscopical, microscopical, leaf constant, powder behavioral with chemical reagents and fluorescence analysis by using yardstick methods. Sensory and macroscopic studies found that leaves are lanceolate, thin, spirally arranged, dark green in colour, with an astringent taste and acute apex. In transverse section, cuticularised epidermis having polygonal cells was found. Mesophyll cells were differentiated into single-layered palisade cells on each surface and 2 to 3 layered spongy parenchyma. Bowl-shaped vascular bundle in mid rib portion containing xylem and phloem tissues. The quantitative microscopy of leaves showed the presence of anomocytic stomata with cicatrix which is an identifying character. The stomatal number and stomatal indexes of the...
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Posted by admin on Dec 31, 2019 in |
A simple, accurate, precise method was developed for the simultaneous estimation of the Ertugliflozin and Metformin in Tablet dosage form. For the method development, Chromatogram was run through HSS C18 (100 × 2.1 mm, 1.7µ) column at a flow rate of 0.3 ml/min. and buffer used in this method was Ortho Phosphoric Acid buffer. The temperature was maintained at 30 °C. The optimized wavelength selected was 240 nm. The retention time of Ertugliflozin and Metformin was found to be 0.736 min and 1.286 min. % RSD of the Ertugliflozin and Metformin were and found to be 0.8 and 0.9 respectively. % Recovery was obtained as 100.98% and 99.81% for Ertugliflozin and Metformin, respectively. LOD, LOQ values obtained from regression equations of Ertugliflozin and Metformin were 0.02 µg/ml, 0.08 µg/ml and 1.04 µ/ml, 3016 µg/ml respectively. The regression equation of validated method for Ertugliflozin is y = 4132.x + 239.4 and y = 3921.x + 4097 of Metformin, respectively. Retention times were decreased so that run time was decreased, the...
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Posted by admin on Dec 31, 2019 in |
Ashwagandhadi lehya is an Ayurveda formulation, official in Ayurvedic Formulary of India and used as aphrodisiac, tonic, rejuvenating agents etc. It is one of the major components of Ashwagandhadi lehya is Withania somnifera which contain Withaferin –A. The study was undertaken to develop an analytical method using HPLC to estimate Withaferin – A, being a marker from, methanolic extract of Ashwagandhadi lehya as one of the quality parameters. Method development involved simple sample preparation without any purification, as well as isocratic separation of Withaferin-A. The method was subjected to validation involving establishing linearity, precision, accuracy, specificity, and robustness.Optimized mobile phase was acetonitrile: water (45: 55 v/v) contained 1% v/v glacial acetic acid. The column used was Phenomenex (250 × 4.6 mm, 5 µm) with flow rate set at 1 ml/min and chromatogram was recorded at 216 nm. The studies showed that peak corresponding to Withaferin – A within concentration range of 1-75 µg/ml was found to be correlated with respective concentration with correlation coefficient (r2) 0.99802 ± 0.0004. The...
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Posted by admin on Dec 31, 2019 in |
The aim of the present study was to formulate an osmotically controlled drug delivery system (O-CDDS) of Metformin hydrochloride to reduce the frequency of multiple dosing in non-insulin dependent diabetes mellitus-II which is a lifelong disease. Metformin hydrochloride a BCS Class-III drug having a poor biological half-life of 6 h. O-CDDS of Metformin hydrochloride is a recent approach for the zero-order release profile. Different factors X1 (effect of percentage of plasticizer), X2 (effect of percentage of release modifier), X3 (effect of percentage of coating) were optimized using central composite design. The granules were prepared by wet granulation technique using PVP K90 as a binding agent. Evaluations of granules like bulk density, tapped density, Hausner’s ratio, carr’s compressibility index, angle of repose was done. Then core tablets were prepared using 16 station tablet press and evaluated for hardness, friability, weight variation. For the preparation of a semi-permeable membrane, the coating of cellulose acetate using polyethylene glycol 400 as a plasticizer was done. Then drilling was done mechanically to create the...
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