Posted by admin on Sep 30, 2020 in |
HPTLC is simple, reliable, precise and accurate for separation and identification of drug in a combined dosage form which gives satisfactory accuracy in results when applied on amlodipine besylate (Amlo) and celecoxib (Celo). Toluene, ethyl acetate, methanol and conc. ammonia (6:5:1.5:0.3 v/v/v/v) was required as a mobile phase for separation and identification of drugs. These drugs were scanned by densitometry at 310 nm. The developed method was validated for linearity, precision, accuracy, LOD, and LOQ. The linearity range and correlation coefficient for Amlo and Celo were obtained to be 100 to 600 ng/ml and 2 to 12 μg/ml, 0.9971, and 0.9989, respectively. The results of the recovery study for Amlo and Celo have obtained the range of 99.52% to 99.83% and 99.83% to 99.98%, respectively. The method precision was established by repeatability study. LOD and LOQ are 17.88µg/ml and 54.20 µg/ml for Amlo and 0.39µg/ml and 1.20µg/ml for Celo, respectively. The statistical parameters and recovery data indicated that the method may be engaged for efficient, rapid drug analysis from...
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Posted by admin on Sep 30, 2020 in |
In the present study, eleven new chalcones bearing a methanesulfonyl group at para position have been synthesized by the condensation of 4-methylsulphonylacetophenone with different para-substituted aromatic aldehydes. The structures of the newly synthesized molecules were elucidated by spectroscopic methods such as as1H NMR, 13C NMR, and mass. These compounds were screened for their anti-inflammatory activity by carrageenan-induced rat paw edema and leucocytes migration methods and analgesic activity by acetic acid-induced writhing test in albino Wistar rats. Among the synthesized compounds, MS7 to MS10 which contain electron-withdrawing groups were found to be most active with reference to anti-inflammatory activity at a dose level of 10 mg/kg and caused a significant reduction in the number of recruited leukocytes and inhibition of pleural exudates formation that was comparable to that of indomethacin at a dose of 10 mg/kg. The compounds which contain halogens and methoxy groups showed a significant analgesic activity at a dose of 10 mg/kg when compared to standard diclofenac sodium. Thus, it can be concluded that among the compounds...
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Posted by admin on Sep 30, 2020 in |
Cerebral ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demands. This leads to poor oxygen supply or cerebral hypoxia and thus leading to the death of brain tissues or cerebral infarction/ischemia stroke. It has been the third most common cause of death in developed countries; stroke can affect walking, talking, speech, vision, spatial awareness, swallowing, bladder control, bowel control, and balance co-ordination. The test drug Amaranthus spinosus leaves were defatted with petroleum ether (60-80 °C) and then extracted with hydroalcoholic solvent (Ethanol 95%, v/v: water, 1:1) by soxhlation process. The hydroalcoholic extract of the drug was evaluated for cerebroprotective activity in Wistar albino rats in the doses of 200 and 400 mg/kg by the Induction of cerebral ischemia/reperfusion followed by behavioural tests, infarct size measurement, and antioxidant activity. The test drug ASE produced the cerebroprotective activity in a dose-dependent...
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Posted by admin on Sep 30, 2020 in |
Medicinal plants are the predominant source of phytochemicals and nutraceuticals, and it is used for the treatment of many diseases for its high effectiveness, low cost, and wide availability. In the current research study five, commonly used ethnomedicinal weeds of West Bengal, and India namely; Heliotropium indicum, Tridax procumbens, Cleome rutidosperma, Commmelina benghalensis, and Euphorbia hirta were used for analyzing their nutritional potentiality measurements. Different solvents extracts of leaves of the plants were used for detecting and quantifying the essential phytochemicals and nutraceuticals. For the quantitative study, UV-Vis spectrophotometer and titration method was used. Mineral content analysis was done by atomic absorption spectrophotometer followed by acid digestion method. To the best of our knowledge, the comparative evaluations of organoleptic, proximate characteristics, and nutritional components of these herbs are reported first. FT-IR study of leaves aqueous extracts reveals different types of functional groups. Among the studied plants, EH leaves extracts showed the highest amount of presence of polysaccharides, amino acids, ascorbic acids, thiamine, and riboflavin. CR leaves extracts showed the...
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Posted by admin on Sep 30, 2020 in |
Seeds of Hygrophila spinosa T. Ander (Acanthaceae) are traditionally used as an aphrodisiac and spermatogenic in the Indian System of Medicine. Studies were planned to evaluate the effect of unsaponifiable (sterol-enriched) fraction from chloroform extract, of the seeds of Hygrophila spinosa as spermatogenic and as aphrodisiac in-vivo, using rats as experimental animals. An unsaponifiable fraction was evaluated for its ability to stimulate testosterone production using isolated rat leydig cells in-vitro. The fraction was administered for 28 days to animals. At the end of the study period, biochemical evaluation of selected parameters, determining the increase in weight of reproductive organs, histological studies of testes, and sexual behavioral studies were performed as evaluation parameters. Increased serum cholesterol and testosterone level, increased weights of reproductive organs, and marked improvement in testes histo-architecture of rats suggested the spermatogenic potential of the fraction. Mounting behavior of test animals improved while the latency period was decreased, as observed in behavioral studies. Stimulation to leydig cells and increased serum testosterone level in-vivo might be responsible for...
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