Posted by admin on Aug 31, 2020 in |
Simple, sensitive, and accurate spectrophotometry method one each for estimation of five drugs viz., chloroquine phosphate (CHP), granisetron hydrochloride (GRA), rizatriptan benzoate (RIZ), zoledronic acid (ZOL) and zolmitriptan (ZOT), have been developed. The method depends upon oxidation of each drug by excess N-Bromo succinimide (NS), and subsequent determination of UN reacted NBS by Rhodamine-B dye at λmax 557 nm. Beers law is obeyed between 2-14 µg mL-1 (for CHP); 52-14 µg mL-1 (for GRA); 1-7 µg mL-1 (for RIZ); 1-7 µg mL-1 (for ZOL) & 2-14 µg mL-1 (for ZOT). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. All the methods have been validated as per ICH...
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Posted by admin on Aug 31, 2020 in |
Alzheimer’s disease is the most common neurodegenerative disease, which initially causes oxidative stresses and leads to neuronal death. It characterized by notable memory loss, cognitive impairment, and personality disorders accompanied by diffuse structural abnormalities in the brain of the aged population. The present work focused on anticholinesterase inhibiting activity Smilax zeylanica and Smilax china against neurodegenerative disorder such as Alzheimer’s diseases on animal model respectively, as proof of preliminary anticholinesterase profile. In this paper, we studied various extracts prepared from roots of Smilax zeylanica and Smilax china for their AChE -inhibitory activity using animals. The ethanolic and various extracts from the plant roots of smilax zeylanica and smilax china were screened for their acetylcholinesterase inhibitory activity by using animals and Ellman’s method. These extracts of smilax china and smilax zeylanica exhibited inhibitory activities were observed (Cortex and Hippocampus) at a concentration of 150 mg/kg, it can be used for further study to treat the neurodegenerative disorders like Alzheimer’s disease...
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Posted by admin on Aug 31, 2020 in |
At present, the whole world fronting a very challenging situation of the pandemic, severe acute respiratory syndrome due to coronavirus (COVID-19). Today, COVID-19 is spreading rapidly in every part of the world; these pandemic affected billions of people. This virus is found as a new human pathogen. However, currently, there is no hasty therapy available, which will provide fruitful results in this regard. As per previously reported data, human convalescent serum (plasma) will be a great choice for the rescue patients from COVID-19 infection, and it will be readily available if there are adequate numbers of people who have recovered and can donate immunoglobulin consisting serum. It includes the administration of antibodies against a given agent to an affected individual for the cause of treating an infectious disease due to the virus. In disparity, active vaccination involves the induction of an immune response that takes time to develop and varies depending on the vaccine recipient. The plasma therapy has a storied history going back and founds as well reported...
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Posted by admin on Aug 31, 2020 in |
The current pandemic of the novel coronavirus (SARS-CoV-2) termed as COVID-19 is the third recorded blowback of an animal coronavirus to a human being in the last twenty years. The pandemic is originated from China and currently impacted more than 205 countries worldwide (as on dated 2nd April 2020). This pandemic is not only affecting human life but also has questioned the worldwide status of healthcare facilities and resulted in a slowdown of the economy. But the fact is the SARS-CoV-2 virus, which causes Covid-19, has less virulence but a high infection rate than SARS-CoV-1, which caused an outbreak of SARS in 2003. The disease is mainly transmitted either through close contact with an infected person via respiratory droplets or by touching a contaminated surface. Treatment involves a combination of certain potent antiviral drugs. SARS-CoV-1 and SARS-CoV-2 share 80 to 90 percent genetic similarity; thus, the treatments which are effective against SARS-CoV-1 can be adjuvant therapeutic strategy against SARS-CoV-2. Some of the essential oils with certain active compounds show...
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Posted by admin on Aug 31, 2020 in |
A simple, sensitive, and rapid Liquid Chromatography-Mass Spectroscopy method was developed and validated for the quantification of Isoproterenol in human plasma using Dobutamine as an internal standard. The method utilized simple liquid-liquid extraction using a mixture of diethyl ether and dichloromethane for the sample preparation involved prior to LCMS analysis. The analytes were chromatographed on Prontosil ODS C18 Column with isocratic elution using methanol, Acetonitrile and Tri ethyl-amine in the ratio of 60:25:15 (v/v) at pH 6.3 as the mobile phase at a flow rate of 0.9 mL/min and the UV detector response of the column eluents were recorded at a wavelength of 248 nm. Quantification was performed in multiple-reaction-monitoring mode with the ion transitions m/z 212.19 → 135.21 for Isoproterenol, m/z 302.19 → 107.05 for Dobutamine. Good linearity was obtained in the range of 0.50–300 ng/mL (r2 = 0.999). The method was fully validated with accuracy, precision, matrix effects, recovery and stability. The results of the stability study confirm that the method was found to be stable. The...
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