Posted by admin on Jul 31, 2020 in |
Black tea, the most widely consumed aromatic non-alcoholic beverage second to water, is prepared by boiling cured leaves of Camellia sinensis. It is reported that black tea contains plenty of bioactive constituents, including complex polyphenols like theaflavins (TF2A, TF2B, TF3) and thearubigins that exhibit a wide range of pharmacological properties and also contribute to its quality and bioactivity. The present study aims at biochemical characterization and evaluation of antimicrobial activity of Theaflavins and Flavonoids rich Extract (TFE) and their silver nanoconjugates. TFE was obtained by steeping black tea in boiling water at 90 °C for 5 min, subsequently extracted with ethyl acetate and dried for further use. The biochemical characterization of TFE was done using HPLC and other spectrophotometric methods. Next, silver nanoconjugates of the TFE were prepared using a green synthesis route, and antimicrobial property was evaluated with respect to its non-conjugated form. It was observed that the TFE is an antioxidant rich fraction and helps in metal-nanoparticle synthesis efficiently. The study highlighted that, in maximum cases, nanoconjugates...
Read More
Posted by admin on Jul 31, 2020 in |
Recently, several methods have been developed for the determination of drugs and their impurities products by Reverse Phase-High Performance Liquid chromatography (RP-HPLC). The present paper describes highly specific, linear, precise, rugged, accurate, robust, and stability, indicating the RP-HPLC method for the determination of related substances present in Venlafaxine tablets as per ICH guidelines. Chromatographic separation of impurities at satisfactory level was achieved at the detection wavelength of 235 nm using column Agilent C18 column (4.6 × 150 mm, 5 µ) and a mobile phase of Methanol: Phosphate buffer of pH 6.8 (60:40% v/v), pumped at a flow rate of 1 ml/min. The % recovery of Venlafaxine and Impurity-A was found to be 98.19% and 98.97%, respectively. The % RSD of Venlafaxine and Impurity-A was found to be 0.52 and 0.83, respectively. The proposed method was found to be specific, linear, precise, accurate, robust, stable, and can be successfully used for the determination of related...
Read More
Posted by admin on Jul 31, 2020 in |
Acute renal failure (ARF) is characterized by a rapid, potentially reversible decline in renal function, including a rapid fall in glomerular filtration rate (GFR) and retention of nitrogenous waste products over a period of hours or days. The mortality rate of patients with ARF has remained 25–70% despite the use of various pharmacologic agents. Due to the multiple causes of renal failure, many animal models have been developed to advance our understanding of human nephropathy. Among the experimental models, rodents have been extensively used to enable a mechanistic understanding of kidney disease induction and progression, as well as to identify potential targets for therapy. Numerous experimental models have confirmed the nephrotoxicity induced by gentamicin, cisplatin, acetaminophen, glycerol, CCl4, adenine, potassium dichromate, and others. Nephrotoxicity induced in these experimental models showed pathophysiological, ultrastructural and functional renal impairments in the form of tubular desquamation and necrosis and elevated blood urea and serum creatinine. The aim of this study was to know the mechanism of actions of different nephrotoxic agents for inducing...
Read More
Posted by admin on Jul 31, 2020 in |
Herbal drugs have gained more importance and popularity in recent years because of their safety, efficacy, and low cost. The pteridophytes have an important role in folklore medicine, although neglected in modern days. These plants have been successfully used in different systems of medicines like Ayurvedic, Unani, Homeopathic, and other systems of medicines. Antimicrobial drugs are the greatest contribution of the present century to therapeutics. Dryopteris cochleata, family Dryopteridaceae which is popularly called as Jatashankari exhibits various therapeutic values to treat many diseases such as, epilepsy, leprosy, wounds, ulcers, diabetes, etc. was selected for the present research work to screen for antibacterial properties. The antimicrobial activity of ethanolic extract of Dryopteris cochleata was screened against two gram-positive bacteria (B. subtilis, S. mutans) and three-gram negative bacteria (E. coli, P. aeruginosa, K. pneumonia) and antifungal activity against Candida albicans and Aspergillus flavus by disc diffusion method. The extract showed significant antimicrobial activity against B. subtilis, S. mutans, E. coli, P. aeruginosa, Candida albicans but did not show antimicrobial activity towards...
Read More
Posted by admin on Jul 31, 2020 in |
Chitosan-based porous scaffolds are of great interest in biomedical applications especially in tissue engineering because of their excellent biocompatibility in vivo, good texture, surface contact, controllable degradation rate and tailorable mechanical properties. These days biomaterials scaffolds have contributed as an alternative choice of therapy mainly due to the increase failure rates in autografts and allografts techniques. Terbinafine HCl is allylamines group of drugs which is used topically to treat dermatophyte group of fungi like ringworm. Chitosan possesses both anti-bacterial and antifungal property which synergises with Terbinafine HCl (TBH) for both prophylactic and therapeutic actions in treating fungal wound infection (FWI). The haemostatic property of chitosan allows sorption of plasma, erythrocyte coagulation and platelets activation. These properties contribute additional role in repairing debilitated tissue. The prolong drug release property of fabricated scaffold and its natural characteristics of remodelling tissue favours its use in human fungal wound dressing. The main intention of this research work is to fabricate stable polymer medical devices, examine drug release profile and characterize TBH loaded CS porous scaffold...
Read More
