Posted by admin on Jul 31, 2020 in |
This research explores Achyranthes bidentata L., Adhatoda zeylanica and Aeginetia indica L’s pharmacological potential. Antibacterial disc assay (20 mg/ml), antifungal disc assay (20 mg/ml) and antioxidant DPPH/reducing energy assay (50 μg/ml, 100 μg/ml and 200 μg/ml) were tested for biological operations of plant extracts (aqueous, methanol and acetone leaf extracts). Significant alkaloid content (378.37 ± 35.02 mg/g) was identified in Aeginetia indica L. with the lowest tannin content in Achyranthes bidentata L. (13.37 ± 3.15 mg/mg). Aeginetia indica L. methanol extract (99.28 ± 10.47%) disclosed significant antioxidant potential (p<0.05). Significant antibacterial and antifungal activity was demonstrated against Escherichia coli (22.80 ± 5.06 mm), Pseudomonas aeruginosa (22.80 ± 5.06 mm), Bacillus subtilis (25.40 ± 5.21 mm), Candida albicans (19.40 ± 2.05 mm) and Aspergillus niger (19.80 ± 1.48 mm) from Aeginetia indica L. methanol extracts. All plant methanol extracts showed significant antibacterial activity (22.80 ± 5.06 mm – 25.40 ± 5.21 mm) and antifungal activity (19.40 ± 2.05 mm – 19.80 ± 1.48 mm) very close to standard medicines whereas...
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Posted by admin on Jul 31, 2020 in |
In the study, we have successfully synthesized N-3 substituted tetrahydroquinazolinone by the reaction of isotonic anhydride with aniline derivatives. Thereafter novel derivative would be subjected to synthesize (1, 2, 3, 4-tetrahydroquinazoline) by Mannich reaction with ciprofloxacin and norfloxacin. These synthesized compounds (2a, 2b, 2c, 2d, 2e, 2f, 2g, and 2h) were characterized by FT-IR, 1H NMR, MASS spectroscopy, and elemental analysis and evaluated for their in-vitro antimicrobial activity and anti-inflammatory activity. The antimicrobial activities of synthesized compounds were evaluated against gram-positive and gram-negative bacteria. Amongst these tested compounds 2c, 2d, 2e, 2f exhibited potent antimicrobial activity than the rest of the compounds. All the synthesized compounds were screened for their anti-inflammatory activity using the carrageenan-induced rat paw edema model and exhibited protection against carrageenan-induced...
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Posted by admin on Jul 31, 2020 in |
The application of nanobiotechnology is an emerging area of nanoscience and nanotechnology. In the present studies, tungsten nanoparticles were synthesized and reduced chemically, and character-rization was done by UV, SEM, TEM, FT-IR, and XRD. The size of nanoparticles was found to be 20 nm. Further, these nanoparticles were teste for various biological applications. In antimicrobial activity, it was observed that potent efficacy was observed against Streptomyces grisveus (12 mm) at 80 µg/ml while in fungus maximum activity was observed against Penicillium funicolusum (24 mm) at the same dose. The antiplatelet activity of nanoparticles was investigated by Prothrombin (PT) and Activated Partial Thromboplastin Time (APTT). In both PT and APTT assay, maximum activity was observed at 40 µg/ml (295 sec and 80 sec, respectively). Cytotoxicity was also studied by MTT assay against various cell lines. Against MCF-7, potent activity was observed at 200 µg/ml, while in 3T3 it was observed at 500 µg/ml. Finally, it was observed that these nanoparticles have potent activity against tuberculosis at 1000 µg/ml. The result...
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Posted by admin on Jul 31, 2020 in |
The present study was aimed to evaluate the in-vitro anti-inflammatory activity of fruits and leaves extract of Cucumis melo L. subsp. agrestis (Naudin) Pangalo (Family Cucurbitaceae). The ethanol and aqueous extracts of fruits and leaves of the plant were subjected to preliminary phytochemical screening and in-vitro anti-inflammatory activity. Extracts were incubated with bovine albumin under controlled conditions for denaturation, and protein denaturation was calculated by determination of their absorbance. Since, the erythrocyte membrane is analogous to the lysosomal membranes and its stabilization implies that the extract may well stabilize lysosomal membranes thus human red blood cell membrane stabilization was used as a method to study the in-vitro anti-inflammatory activity. The results showed that the plant extracts showed anti-inflammatory activity in a concentration-dependent manner, and the activity was increased on increasing the concentration of extracts. The ethanol extract was found to be more effective than other extracts of fruit and leaf. The present study reveals that the fruit of Cucumis melo L. subsp. agrestis (Naudin) Pangalo possesses a higher anti-inflammatory...
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Posted by admin on Jul 31, 2020 in |
In the present study, we had developed Asenapine maleate (ASNM) loaded microemulsion (ME) to increase the solubility of Asenapine maleate by components of microemulsion including formulation concern, characterization, mucosal diffusion, stability and nasal ciliotoxicity of Asenapine maleate microemulsion (AME). For nasal delivery of Asenapine maleate, a challenge existing in formulation development is the solubilization of poorly water-soluble Asenapine maleate. The intrinsic solubility of Asenapine maleate is about 0.0312 mg/ml. The purpose of this study was to improve the solubility and to enhance the brain uptake of Asenapine maleate through an o/w microemulsion, with suitable intranasal delivery. The optimal microemulsion formulation consisted of Oleic acid, Tween 80: Propylene glycol (PG) (3:1) and water, with a maximum solubility of Asenapine maleate and no ciliotoxicity, was developed and characterized. AME was characterized for % Transmittance, pH, viscosity, globule size, zeta potential, drug content with in-vitro release studies and Ex-vivo diffusion studies using Standard Franz Diffusion cell. Further, the behavioural studies were assessed in rats by inducing hyperactivity. The optimized microemulsion was found...
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