Posted by admin on Jul 31, 2020 in |
In the healthcare system, the development and modernization of phytomedicine in phytochemistry play a central and important role in drug development. Isolation and purification of bioactive compounds from naturally occurring substance are of prime importance. A sensitive and reliable high-performance thin-layer chromatography method has been developed for the estimation of gallic acid in the alcoholic extract of Eulophia ochreata Lindl. Alcoholic extract prepared was applied on silica gel G 60 F254 plate. The plate was developed using toluene: ethyl acetate: methanol: formic acid (6:6:0.4:1.6) as a mobile phase detection and quantification were performed by densitometric scanning at 275 nm. The system was found to give well-resolved bands for alcoholic extract having Rf values as 0.8 was matched with the standard Rf values like 0.82. The densitometric chromatogram of HPTLC fingerprint of the alcoholic extract, isolate, and standard gallic acid was obtained. The calibration curve of gallic acid was linear over a concentration range (0.2- 2 microg/ml) with a good correlation coefficient (R2= 0.9986) and coefficient of variation as CV-...
Read More
Posted by admin on Jul 31, 2020 in |
The compounds that are derived from the natural sources have been used for the treatment of cancer since ancient times. The tubers of Eulophia nuda, belonging to family Orchidaceae, have been reported for its anti-cancer use in the literature. There are many disadvantages to the conventional herbal drug delivery system. These disadvantages could be overcome by formulating these herbs using novel drug delivery systems. Microemulsions are the novel formulations that improve the solubility and bioavailability of herbal bioactive bio-active compounds. In the present study, microemulsion loaded with alcoholic extract of tubers of E. nuda has been formulated by the water titration method using oil (Oleic acid), surfactant: cosurfactant (Smix) (Tween 80 and PEG400) and water. The prepared formulations of microemulsions were subjected physicochemical characterization parameters such as globule size, percentage transmission, refractive index, viscosity, pH, drug loading, and percentage drug permeated. The optimized microemulsion was further subjected to an in-vitro cytotoxicity study by MTT assay using the MCF7 breast cancer cell line. The optimized microemulsion loaded with alcoholic extract...
Read More
Posted by admin on Jul 31, 2020 in |
Notable progress has been made in methods that encourage the use of PCR as a rapid and accurate tool in quality evaluation of pharmaceuticals. In this study, monoplex and multiplex PCR based assays were developed and compared with standard conventional methods for rapid detection of three specified topical indicator pathogens, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans, in nonsterile pharmaceutical preparations. The detection limit of monoplex PCR assays for the microbial targets was achieved at 100 fg purified DNA and 10 CFU/ml for Pseudomonas aeruginosa and 1fg purified DNA and 10 CFU/ml for each of Staphylococcus aureus and Candida albicans. No change in the detection limit for cfu/ml of the three tested indicator pathogens was obtained upon using mPCR assays. The results of applying both conventional and PCR detection methods for different cream and lotion preparations revealed a 100% correlation between both methods. The PCR based detection method can be completed in 8 h versus 5-6 days in case of conventional methods, but the former can’t differentiate between viable...
Read More
Posted by admin on Jul 31, 2020 in |
Antimicrobial drug resistance is one of the major threats due to the wide usage of broad-spectrum antimicrobial drugs in the general population. The present study was undertaken to investigate the antibacterial potential of Alternanthera philoxeroides methanolic extract (APME) against multidrug-resistant UTI pathogens. The preliminary phytochemical screening of the extract showed the presence of carbohydrates, amino acids, proteins, alkaloids, glycosides, steroids, flavonoids, tannins, and phenolic compounds. The quantitative estimation of total phenolic, tannin, and flavonoid contents was determined for the crude extract of APME through standard curves of gallic acid and rutin. The phenolic content of APME was found to be 12.4 mg of gallic acid equivalent (GAE)/g of extract. The tannin content was found to be 5.6 mg of GAE/g of extract, whereas the flavonoid content was found to be 3.2 mg of rutin/g of extract. The antibacterial potential of the extract was observed as maximum against Staphylococcus saprophyticus, moderate against Escherichia coli and Proteus vulgaris, and minimum against Proteus mirabilis. The minimum inhibitory concentration was ranged from 12.5...
Read More
Posted by admin on Jul 31, 2020 in |
Liver diseases are a worldwide problem. Conventional drugs used in the treatment of liver diseases are sometimes inadequate and can have serious adverse effects like mild digestive problem, headache, nausea etc. It is necessary to search for alternative drugs and for treatment of liver disease to replace currently used drugs of doubtful efficacy and safety. Herbal therapies are free from side effects and toxicity, unlike allopathic medicines. Ayurvedic drugs are also acts by multiple pathways and shown full protection in liver disorders. Composition of marketed formulation Livsplin syrup (Test A) and Li- verve tonic (Test B) containing different plant extracts reported hepatoprotective and antioxidant activities. Thus these formulations may be synergistically or additively may show beneficial hepatoprotective activity with allopathic medicines. Hence, it may useful as alternative medicine to cure different kinds of liver diseases in man and animals. From our investigation, results were shown that administration of Li – verve tonic effectively protected against the loss of antioxidant activities of SOD, CAT, MDA respectively compared to control group...
Read More
