Posted by admin on Apr 30, 2020 in |
The present study describes simple, accurate, precise and cost effective UV-spectroscopic method for the estimation of clomiphene citrate in methanol as per ICH guidelines. The absorption maxima for clomiphene citrate in methanol was found to be 235nm and 294 nm. The drug follows linearity (linearity 1, linearity 2 & linearity 3) in the concentration range 3-40 μg/ml at 294 nm and 3-24 μg/ml at 235 nm with a correlation coefficient value of 0.9991, 0.9989, 0.9988 and 0.999, 0.9994, 0.9994 respectively. The accuracy of the method was checked by recovery experiment performed at three levels i.e., 80%, 100%, 120%. The percent recovery was found to be in the range of 90-120%. The low values of % RSD are indicative of accuracy and reproducibility of method. The % RSD <2 indicates that method is precise. The ruggedness of method was studied with the help of different analysts. No significant degradation of clomiphene citrate was showed in force degradation studies like heat-induced degradation the spectra for acid degradation, acid/base hydrolysis, oxidation and...
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Posted by admin on Apr 30, 2020 in |
Chalcones are naturally occurring compounds with a wide range of biological activities along with an anticancer activity through various mechanisms. It is also known as aromatic ketone, which creates a central core for different essential biologically active compounds. The literature on anticancer chalcones shows the use of various prolonged approaches like structural manipulation and replacement of both aryl rings, molecular hybridization by combination with other pharmacological important moieties to enhance anticancer characteristics. Methoxy substitution on both the chalcones A and B aryl rings based on their position in the aryl rings, usually affect anticancer and other activities. Additionally, chalcones affect the anticancer activity proven through the various categories of compounds. Hybrid chalcones developed by chemically linking chalcones to other leading anticancer scaffolds such as benzodiazepines, imidazole, benzothiazole have proved complementary or intrinsic pharmacological behavior. This study summarizes the concerted attempts made in the design and growth of anticancer chalcones reported in the latest literature and also gives an overview of recently released...
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Posted by admin on Apr 30, 2020 in |
The main aim of this work was to formulate and study mucoadhesive buccal tablets of valsartan using various suitable bioadhesive polymers such as CP 934 and Xanthan gum. Nine formulations of valsartan were prepared by the direct compression method. The prepared tablets were characterized by swelling studies, % matrix erosion, surface pH, bioadhesive properties, in-vitro drug dissolution and in-vitro diffusion studies. It was found that CP 934 increases the swelling index also increased. The surface pH of all formulations was found to be satisfactory, and values were in between the range of 5-7 pH, hence no irritation to the buccal cavity is assumed. Tablets containing CP: Xanthan gum in the ratio 3:1 has shown the maximum percentage of in-vitro drug release through the buccal mucosa. The drug release was found to be zero-order release. The formulation F3 was considered as the optimized formulation based on satisfactory bioadhesive strength, in-vitro dissolution drug release of 96.72 ± 0.43%, for 12...
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Posted by admin on Apr 30, 2020 in |
Diclofenac is the most popular and widely prescribed non-steroidal anti-inflammatory drug (NSAID). However, no preclinical data are available on repeated diclofenac IV bolus injection. In the present study, forty male Wistar rats were randomly divided in in total of four groups with 10 rats in each group. The control group received 0.9% sodium chloride injection. Marketed injectable Diclofenac Sodium formulations of Jonac AQ (75 mg/mL), Dynapar AQ (75 mg/mL) and Voveran (75 mg/mL) were administered in group 2, 3 and 4 respectively @ 15.4 mg/kg body weight as IV bolus injection daily for seven days in the lateral tail vein. At the end of seven days treatment period, six animals per group were sacrificed. The remaining four animals per group were sacrificed after seven days recovery period. On day 8 of the study, 9/10 rats in the Voveran group, 10/10 rats in the Dynapar AQ group and 6/10 rats in Jonac AQ group showed an injection site reaction. On day 15 (reversal necropsy) of the study, 4/4 rats in...
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Posted by admin on Apr 30, 2020 in |
The present study was carried out to characterize the bioactive phytoconstituents from the fractions of F. elephantum fruit and to evaluate their in-vitro anti-diabetic activity. Column chromatography of methanol extract of F. elephantum fruit yielded Hexane: Ethyl acetate (1:1 v/v) fraction (HEFE), Ethyl acetate fraction (EFE) and Ethyl acetate: Methanol (1:1 v/v) fraction (EMFE), which were subjected to GC-MS analysis. They were also tested for in-vitro α-amylase and α-glucosidase inhibitory potential. GC-MS analysis of EFE predominantly showed 2,5-Furandione, dihydro-3-methylene; n-Hexadecanoic acid; 5-Eicosene,(E)-; cis-13-Octadecenoic acid; and γ-Sitosterol; 2,5-Furandione, dihydro-3-methylene-; cis-Aconitic anhydride; Ethanol, 2,2′-[(1-methylethyl)imino]bis-; and Propanedioic acid, ethyl-, diethyl ester; were the major compounds in EMFE. HEFE showed 2,5-Furandione, dihydro-3-methylene (18.5%), Dodecanoic acid (4.48%), n-Hexadecanoic acid (15.18%) and cis-13-Octadecenoic acid (18.95%) which are biologically active. Moreover, the α-amylase IC50 values of HEFE, EFE, and EMFE were 68.77, 52.59, and 40.28 μg/mL, respectively, while that of acarbose was 41.99 μg/mL. And the α-glucosidase IC50 values of HEFE, EFE, and EMFE were 69.53, 35.08, and 42.49 μg/mL, respectively, which were comparable to that...
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