Posted by admin on Apr 30, 2020 in |
The present project was conducted with the objective of developing and validating a RP-HPLC method for the simultaneous estimation of aspirin, clopidogrel bisulphate, and rosuvastatin calcium in fixed-dose combination capsule. The chromatographic separation was carried out on Agilent 1260 series using Waters C18 (250 × 4.6 mm, 5 μ) column as the stationary phase and acetonitrile (ACN): phosphate buffer pH 3, gradient mode at a flow rate of 1.2 ml/min and detection at 230 nm. The validation of the developed method was conducted as per the ICH guidelines Q2 (R1). The retention time of aspirin, rosuvastatin calcium and clopidogrel bisulphate was found to be 3.2 min, 4.7 min, and 12.8 min, respectively, under the optimized chromatographic conditions. The developed method was linear in the concentration range of 6.25-400 μg/ml for aspirin, rosuvastatin calcium, and clopidogrel bisulphate. The developed method was specific, with a mean percent recovery of the three drugs in the range of 99-101%. The relative standard deviation (RSD) was less than 2 in the intraday and inter-day...
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Posted by admin on Apr 30, 2020 in |
Glioblastoma (GBM), with restricted therapy alternatives, is a catastrophic primary brain tumor. The receptor of the epidermal growth factor receptor (EGFR) in glioblastomas is recurrently enhanced, over articulated, or mutated, but up to 20 percent of GBM patients find it to be responded to kinase inhibition of EGFR. Several inhibitors of EGFR tyrosine kinase (TKI) failed clinically, due in part to acquired resistance. To automatically examine this type of resistance, we used molecular docking and swissADME approach to elucidate its putative inhibitor. We have attempted to determine a drug candidate in the current research based on the discovery of structural drugs. Docking simulation was conducted on mutated EFGR to determine the best drug candidate from Erlotinib, a renowned anti-cancer agent, derivatives. A total of 200 structures were selected for the 2D crystal structure of erlotinib based on molecular fingerprinting. Top 10 best-docked proteins were analyzed using UCSF Chimera and discovered the complicated atomic-scale properties between ligand and the target protein. SCHEMBL13087058 ligand selected based on hydrogen bonding with methionine...
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Posted by admin on Apr 30, 2020 in |
Background: Medication errors (MEs) are the potential to cause adverse clinical outcomes. The incidence of MEs is hopefully controlled with patient-centric pharmaceutical services. The present study evaluated the incidence of MEs in various departments in a multispecialty hospital in India. Methods: This prospective observational was analyzed 100 prescriptions included 749 drugs from general medicine, cardiology, renal, and endocrinology departments. The prescriptions were analyzed for medication errors as per NCCMERP criteria. The outcome was expressed as range additionally as percentages of variables. Results: A total of 217 MEs (n-105, 48.39%) were ruled out from out of these 100 cases. The applied NCCMERP criteria found 87 drug administration errors (40.09%) with higher categories A and B, but the distribution of category C (n-10) and D (n-10) was noted quite alarming. Conclusion: The study would suggest perpetual inculcation and training programs to be conducted to incorporate cognizance and practice inputs of medicos, nurses, and...
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Posted by admin on Apr 30, 2020 in |
The present research work was aimed at the enhancement of solubility and dissolution rate of Gefitinib by Self Emulsifying Drug Delivery Systems (SEDDS). Gefitinib is a BCS class II drug with poor aqueous solubility (oral bioavailability 60%). The saturated solubility of Gefitinib in different oils, surfactants and co-surfactants were determined. The excipients were screened and selected showing maximum solubility and compatibility for Gefitinib. SEDDS formulations of Gefitinib were developed using selected Oils, Surfactants and Co-Surfactant combinations (4:1 and 3:1). Pseudo ternary phase diagrams were created using Triplot V 4.1.2 software and applying Pseudo ternary phase diagrams, the nano-emulsification region was identified. Formulations were optimized based on Pseudo ternary phase diagrams using various proportions of oil (Peceol), surfactants (Kolliphor HS 15) and co-surfactants (Labrasol). The prepared four formulations were selected among them F1 was optimized and carried out for further evaluations like robustness to dilution (Passed), dispersibility test (Grade A), self emulsification time (29 ± 1.05 sec), percentage transmittance (Clear emulsions), drug loading efficiency (98.735 ± 0.43%), thermodynamic stability study...
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Posted by admin on Apr 30, 2020 in |
Candida albicans is the leading cause of superficial and systemic fungal infections in human beings and is very common in people with a weakened immune system. It can grow either as budding yeast, a pseudohyphae form, or in a true hyphal form, and the ability to switch between these three forms is important for its pathogenicity. To date, an in-depth study on the relationship between histone modification and hyphal formation is limited, and our study has therefore aimed at adding further information regarding this relationship. In our study, we have found that by disrupting the gene GCN5that codes for a histone acetylating protein Gcn5, there was indeed a defect in the hyphal formation confirming the previous report made by Chang et al., (2015). We also found that the defect in the hyphal formation was probably due to regulation of hyphal inducing signaling protein Ras1 by Gcn5 leading to its reduced expression as well as its downstream target hyphal transcription factors Efg1 and Cph1 in the homozygous mutant. The reduced...
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