Posted by admin on Feb 28, 2021 in |
Ethnomedicinal plant-like Gymnosporia montana belonging to the family Celastraceae is commonly known as Vikalo in Gujarat. Ethanomedicinally, fresh leaves of Vikalo are chewed in trible regions of Gujarat to cure jaundice. Phytochemical screening of the plant showed the presence of phytoconstituents like phenol, flavonoids, alkaloids, triterpenoids, and saponin. The present study was undertaken for Phytochemical Investigation, Isolation and Characterization of Betulin from the leaf of Gymnosporia montana. On the basis of characterization studies, the novel compound betulin was isolated by column chromatography; the isolated compound has been evaluated for its physical properties (colour, state, solubility, melting range, and Rf value), which identically resembles the standard...
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Posted by admin on Feb 28, 2021 in |
This study reports the isolation and antioxidant activity of non-polar terpenes extracted from the leaves of Bougainvillea glabra choicy. To characterize the phytochemical constituents of Bougainvillea glabra leaves using the GC-MS method. The 95% ethanol extract was filtered, distilled, and concentrated to obtain the solid greenish residue. The residue was further fractioned by n-hexane, chloroform, ethyl acetate, acetone, ethanol, and methanol. The GC-MS analysis of Chloroform extract of Bougainvillea glabra leaves revealed the presence of thirteen compounds that could contribute to the medicinal quality of the plant. The first compound identified with less retention time (9.50 min) was 1-(1-methyl-decyl)-decahydro-napthalene. The isolation of organic compounds was done using column and thin layer chromatographic techniques. Compound characterization using various spectroscopic techniques identified the final isolated compound as 1-(1-methyl-decyl)-decahydronapthalene. Antioxidant activity was evaluated by the DPPH method. The method of isolation is simple, cost-effective, and...
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Posted by admin on Feb 28, 2021 in |
The aim of the present work is to formulate and characterize Econazole nitrate (EN) loaded transfersomal gel for antifungal activity. EN is a broad-spectrum imidazole antifungal agent that belongs to BCS Class II. Due to poor solubility, EN is incompletely absorbed after oral administration and bioavailability vary among individuals. Topical treatment of fungal infections is usually preferred, but the barrier is to cross stratum corneum, so formulating the drug in Transfersomes (TFs) solved this problem. TFs are a highly adaptable, stress-responsive, complex aggregate, and self-regulating membrane. EN loaded transfersomes were prepared by a thin-film hydration method. The EN-loaded transfersomes were optimized by the three factors and two levels Box-Behnken design using Design-Expert software (version 12). Independent formulation variables such as concentrations of phospholipid and two edge activators were evaluated. The prepared TFs were evaluated with respect to particle size, % entrapment efficiency, and % drug release. The prepared EN loaded TFs particle size ranging from 0.28 to 0.71 µm, entrapment efficiency in between 31.5 to 75%, and drug release...
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Posted by admin on Feb 28, 2021 in |
RP-HPLC method has been developed for separation and quantification of Metoprolol succinate, Telmisartan, and Clinidipine using Phenomenex Luna C18 column (150 mm × 4.6 mm, 5 µm) as a stationary phase with security guard cartridge C18 (4 × 3 mm) and acetonitrile: methanol: phosphate buffer pH 7.5 (45:30:25) as a mobile phase by isocratic elution at the flow rate of 1.0 ml/min. The detection was carried out at 229 nm. The drugs are eluted at retention times 2.0, 2.8, and 6.8min for telmisartan, metoprolol succinate, and clinidipine, respectively. The method is linear in the range of 10-80 μg / ml, 6.25-50 μg / ml, and 2.5-20 μg / ml with regression coefficients 0.997, 0.995, and 0.999 respectively for telmisartan, metoprolol succinate, and clinidipine. The method is precise within the acceptable limits (% RSD- 1.22). The proposed method showed linearity, accuracy, precision, specificity, robustness, LOD, LOQ, and system suitability results within the acceptance criteria of ICH. The method can be applied for the routine analysis of pharmaceutical...
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Posted by admin on Feb 28, 2021 in |
Tinospora cordifolia is one of the best medicinal plants holds most prominent place in Ayurveda Rasayana herbs and it also known as “rejuvenate herbs” because of its medicinal properties. It has been found to exhibit anti-microbial properties, its anti-microbial feature found in root, stem, and leaf extracts on pathogenic micro-organisms. Green synthesis of AgNPs can potentially eliminate chemical agents’ problems that may have adverse effects, thus making nanoparticles more compatible with the eco-friendly approach. The present study focuses on the green synthesize of silver nanoparticle, which was conducted by Tinospora cordifolia leaf extract. Characterization of silver nanoparticle was performed by Scanning Electron Microscopy. Silver nanoparticles exhibit strong anti-microbial effect against gram-positive and gram-negative bacteria such as Escherichia coli, Pseudomonas syringae, Staphylococcus aureus and Enterococcus faecalis. Both methanolic and ethanolic extract showed good antimicrobial activity against all the bacteria whereas methanolic extract had wide range of anti-microbial activity than ethanolic...
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