Posted by admin on Oct 31, 2021 in |
This study is focused on evaluating the detrimental factors of drinking water and its relation to blood glucose level and anxiety status of non-stressed experimental rats. The study was conducted on 30 adult Wistar albino rats. The control group was treated with filtered water by reverse osmosis unit, and test 1, test 2, test 3, and test 4 groups were treated with well water from rural area 1, rural area 2, urban area 1, and pipeline water from urban area 2 respectively. Each week, body weight and fasting blood glucose (FBG) levels were measured. From the 11th week onwards, animals’ anxiety response was measured using an elevated plus maze (EPM). On the 80th day of the experiment, all the animals were sacrificed, and blood was collected, and the pancreas was removed for further investigations. On 9th week of the experiment, FBG level became 83 ± 3.89 mg/dL, 94.6 ± 10.05 mg/dL, 79.8 ± 2.54 mg/dL, 79.5 ± 4.51 mg/dL, 107 ± 6.14 mg/dL for control, test 1, test 2,...
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Posted by admin on Oct 31, 2021 in |
Actino bacterium Streptomyces sp. NLKPB45, which was isolated from mangrove soil sediments of Nellore coastal regions of Andhra Pradesh, India, was used in shake-flask fermentation to explore the different carbon and nitrogen substrates for antimicrobial crude compound. Careful experimentation of ranging each variable in the fermentation medium found that glucose and peptone as preferred carbon and nitrogen sources at 1% (w/v) concentration each, at pH of 7 and 28 °C incubation temperature for 8 days of incubation time. Response surface methodology-based statistical optimization of the process variables with their interconnection was analyzed using a central composite design. A second-order quadratic polynomial equation yielded a complacent fit for experimental with respect to crude compound concentration. Analysis of variance statistics showed that significant-high R2 value for model and adjusted and predicted; R2 values were also shown decent agreement with observed and predicted results. The results have confirmed very good interaction among five process variables at optimized values of 7.51318 pH, 28.734 °C incubation temperature, 8.18409 days incubation time, 1.05185 g/L glucose...
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Posted by admin on Oct 31, 2021 in |
Objectives: The present study aimed to develop simple and accurate first and second-order derivative spectrophotometric methods (1D and 2D) for the assay and stability studies of Ranolazine (RAN) in bulk and dosage forms. Methods: The original UV spectrum of RAN solution in methanol was differentiated instrumentally to generate the first and second derivative spectra which were measured at 278 nm and 283 nm, respectively. The developed methods were then validated as per ICH guidelines and applied for the stability studies. Results: Beer’s law was found to be valid over the concentration range 100-600µg/ml with a correlation coefficient (r) not less than 0.999. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 24µg/ ml and 73 µg/ ml, 17.8 µg/ ml and 53.6 µg/ ml, for 1D and 2D respectively. Good results were also obtained from the assay of RAN tablets (98.6 ± 2.3%, n=3). Studying the stability behavior of RAN using the developed methods reflects its instability under alkaline conditions following the first-order kinetics....
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Posted by admin on Oct 31, 2021 in |
By HPTLC method, a mobile phase system comprising of Ethyl acetate: Toluene: Methanol (5:4:1v/v/v) was selected, and the detection was carried out at 280nm. The Rf values were found to be 0.65 ± 0.03 and 0.27 ±0.03 for Mebendazole and Atorvastatin, respectively, which showed a good separation. The method was validated as per ICH guidelines. The percentage RSD values of repeatability, intraday, and interday were found to be less than 2 prove the precision of the method. The correlation coefficient value from the calibration graph was found to be 0.9949 for Mebendazole and 0.9979 for Atorvastatin. The percentage protein binding of Mebendazole and Atorvastatin was estimated by the equilibrium dialysis method. The in-vitro displacement interaction study was carried out using standard solutions of a mixture containing 1.5×10-4 M Mebendazole and Atorvastatin along with 1.5×10-4 M BSA. The percentage protein binding of individual Mebendazole and Atorvastatin after 6hr was found to be 86.14% and 91.18% respectively. While they were evaluated in the presence of each other, the percentage protein binding...
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Posted by admin on Oct 31, 2021 in |
The main objective of the study was to formulate and evaluate solid self nano emulsifying drug delivery systems (SNEDDS) of famotidine (BCS class-IV drug), a histamine receptor antagonist used in case of ulcers, Zollinger-ellison syndrome. Liquid SNEDDS was formulated using oils like oleic acid, arachis oil, surfactants like Tween 20, Tween 80, etc., and co-surfactants like Transcutol-P, polyethylene glycol. Based on solubility studies, oleic acid (15mg/ml), Tween 20 (38.41mg/ml) & Transcutol-P (2.593mg/ml) were further studied by phase titration method using different ratios of oil: s-mix (surfactant: co-surfactant) from 1:9 to 9:1 with pseudo-ternary phase diagrams. FTIR spectra indicated drug excipient compatibility. Further evaluation studies, namely thermodynamic stability studies, robustness to dilution, self-emulsification time, dispersibility test, drug content, and in-vitro drug dissolution tests, formed the basis for optimization of liquid SNEDDS formulation. In-vitro drug release of F1 (97.15±0.02% in 90 min) and F19 (100.23±0.1% in 60 min) was significantly higher when compared to the pure drug (18.41±0.01% in 90 min). The droplet size of F19 (ratio of 3 (oil): 1...
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