Posted by admin on Oct 31, 2021 in |
Currently, the knowledge on the oncogenic behaviour exhibited by microbial agents is the result of long and laborious studies conducted over the years. It started with the liver fluke infection associated with bile duct carcinoma and bilharziasis with bladder cancer. Following this, multiple pathogens got implicated in carcinogenesis, including bacteria, many viruses, and even fungi. For some of these infections, the cancer-causal association has confirmatory evidence, but the data is limited for others. There is a need to understand the pathogenesis of these infectious cancers and the oncogenic mechanism of newly implicated infectious agents being added to the list of infectious cancer. Cancers arising out of infections are theoretically preventable either by vaccination or early treatment and careful drafting of public health policies can help to reduce the cancer burden. The concept of herd immunity in cancer prevention is new and emerging, but as it has an established role in preventing some infectious diseases, the same advantage can be conferred in the prevention of infectious cancers. Cancer research has...
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Posted by admin on Oct 31, 2021 in |
Objective: To elaborate and characterize new co-processed excipients for direct compression use. Methods: The new co-processed excipients were obtained with different proportions of microcrystalline cellulose (Avicel® PH101) and Isomalt (galenIQ® 800), using Starch® 1500 and Plasdone® K-25as binding agents in a fluid bed. Rheological characterization was performed; to determine their deformation pressure (Py), the Heckel model was applied. Tablets were elaborated with each polymer and each co-processed compound at different compaction pressures, and the behavior of the tensile strength curves was analyzed. Results: The range of particle size of the obtained co-processed compounds was 95.5 to 151µm. The flow velocity of the co-processed excipient elaborated with Avicel® PH101/galenIQ® 800 improved as compared to the velocity presented by the individual raw matters. Conclusions: The Avicel® PH101/galenIQ® 800 co-processed excipients elaborated at 60/40% and 70/30% proportions can be recommended to be used as direct compressible excipients because these proportions presented the best characteristics regarding flowability, less deformation pressure (Py) and a higher slope in the tensile strength...
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Posted by admin on Oct 31, 2021 in |
The plants Portulaca oleracea and Gomphrena globosa belong to the order Caryophyllales, and their flowers are known to produce betalains. The biosynthesis of betalains involves various intermediate molecules such as catecholamines or biogenic amines. Catecholamines include dopamine, adrena-line (epinephrine), and noradrenaline (norepinephrine) and are normally believed to be released in response to stress. They are derived from the amino acid tyrosine, with L-DOPA being formed as an intermediate. Catecholamines and their predecessors (tyrosine and L-DOPA) have many medicinal applications and are used in the treatment of Parkinson’s disease, hypotension, low cardiac output, glaucoma, and allergic conditions like asthma, to name a few. These biogenic amines and their predecessors have been found in many plant families. Their presence has been reported in Portulaca oleracea and Gomphrena globosa. The current study focuses at the identification and quantification, by LCMS/MS, of the different catecholamines or their predecessors that are present in Portulaca oleracea and Gomphrena...
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Posted by admin on Oct 31, 2021 in |
Natural products from various medicinal plants, in the form of pure compounds or standardized extracts, which provide unlimited opportunities for discovery of new drug leads, is due to the unmatched availability of chemical diversity. There is an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, interest, particularly in edible plants, has grown throughout the world. Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. The focus of this paper is on the analytical methodologies, which include the extraction, isolation, and characterization of active ingredients of bioactive compounds from Stachytarpheta urticifolia. The common problems and key challenges in the extraction, isolation, and characterization of active ingredients of bioactive compounds are discussed. As extraction is the most important step in the analysis of constituents present in herbal preparations, the strengths and weaknesses of different extraction techniques are discussed. The analysis of bioactive compounds present in the plant extracts involving the applications of common phytochemical screening assays, chromatographic techniques such as HPLC...
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Posted by admin on Oct 31, 2021 in |
L-glutaminase [EC.3.5.1.2] is an amidohydrolase that catalyzes the hydrolytic deamination of L-glutamine, resulting in the production of L-glutamic acid and ammonia. The L-glutaminase has received a significant attention due to its potential as an anticancer agent. In the present study, soil sediments were collected from the estuaries region of Parangipettai, Cuddallore District, Tamil Nadu. The isolated fungal strains were screened for L-glutaminase production, and the potent strain was characterized and identified as Aspergillus flavus JK-79 (Genebank accession number MF445235). The anti-cancer enzyme L-glutaminase production by Aspergillus flavus JK-79 was evaluated in five different production media under the submerged fermentation process. The Minimal medium showed maximal enzyme production by the marine isolate. Further, parameters such as Inoculum size, pH, temperature, and nutritional requirements such as carbon sources, nitrogen sources, and amino acids were optimized by One-factor-at-a-time (OFAT) for maximal production of L-glutaminase under submerged fermentation process. The maximum production of L-glutaminase by Aspergillus flavus JK-79 was recorded at pH 8 (790 .436U/mL), temperature 28°C with 2% inoculum size. The findings...
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