Posted by admin on Sep 30, 2021 in |
The present study aimed to develop a reversed-phase high-performance liquid chromatographic method for the simultaneous quantitation of water-soluble vitamins like ascorbic acid (C), thiamine (B1), riboflavin(B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6) and folic acid (B9) in ten potent wild edible fruits named Artocarpus gomezianus, Baccurea sapida, Cyclanthera pedata, Flacourtia jangomas, Gymnopetalum cochinchinense, Hodgsonia heteroclite, Prunus nepaulensis, Spondias axillaris, Terminalia bellirica and Zhanthoxylum armatum consumed by the tribal people of North-eastern region in India. The chromatographic separation of vitamins was carried out on Acclaim C 18 column (5 μm particle size, 250 x 4.6 mm), Dionex Ultimate 3000 liquid chromatography, and detection was carried out at three different wavelengths (210, 245 and 254 nm) using a mobile phase of acetonitrile and aqueous trifluoroacetic acid (TFA, 0.01% v/v) solution with gradient elution. The experimental results showed that for different plants, the vitamin C content ranged between0.05 ± 0.001to 4.99 ± 0.03 mg/100g dry plant material (DPM). The B2 content was determined high in F. jangomas (0.62 ± 0.013 mg/100g...
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Posted by admin on Sep 30, 2021 in |
Rasagiline Mesylate (RM), an antiparkinson drug, belongs to BCS class III drug having high solubility with low permeability. It undergoes extensive first-pass metabolism with oral bioavailability of only 35%. In the present research work, efforts were made to improve its permeation as requisite for the transdermal drug delivery system. RM was authenticated by determination of melting point. Further confirmed by ATR-FTIR, DSC and XRD. The solubility and pH dependant solubility profile was established. The purity of the received drug and estimation of the drug during the study was performed by an in-house developed novel RP-HPLC method. To determine the drug flux, permeation experiments were performed in modified Franz diffusion cells using dialysis membrane and excised rat abdominal skin as a barrier and the flux found to be 45.4 and 29.9 µg/cm2 / h, respectively. To increase flux and permeation, permeation enhancers with different mechanisms like Isopropyl myristate (IPM), Hyaluronidase, Ethanol, Oleic acid, Dimethyl sulfoxide (DMSO), Polyethylene glycol (PEG 400) and Propylene glycol (PG) were employed in the concentration of...
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Posted by admin on Sep 30, 2021 in |
Green synthesized gold nanoparticle is one of the most promising drug delivery approaches with biocompatibility and biodiversity. Various plant sources such as Aegle marmelos, Eugenia jambolana, Soursop, Persea Americana, Terminalia chebula, Aloe arborescens, Musa paradisiacal, Alternanthera philoxeroides, Cissus quadrangularis, Sterculia acuminate, Garciniaindica choissy, Eucalyptus globulus, Rosmarinus officinalis, Punica granatum, Pistacia atlantica, Pistacia integerrima, Juglans regia, Curcumae kwangsiensis; fungal sources as Pleurotus cornucopiae var. citrinopileatus, Cladosporium cladosporioides; other microbial sources as Magnusiomyces ingens LH-F1, Micrococcus yunnanensis, Padina tetrastromatica are used to develop biocompatible gold nanoparticle with veritable diversified particle size and applicability profile as anticancer (especially against breast cancer, liver cancer, ovarian cancer and lung cancer), antibacterial agent, photo luminescent, heavy metal sensor, etc. If biogenic sources are composed of a large number of hydroxyl and carboxylic acid groups, it can behave as reducing agents to develop gold nanoparticles with immense biomedical and pharmaceutical applications. This novel approach and data are very much encouraging and may be considered as one platform for searching all the important green synthesized gold nanoparticles...
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Posted by admin on Sep 30, 2021 in |
The present study investigates the isolation and identification of Multi-Drug Resistant bacteria from Hospital Effluent and the determination of Antimicrobial activity of Terminalia bellerica (Roxb.) against the isolated Multi Drug-Resistant Bacteria. Hospital effluent was collected and screened for the presence of Drug resistance phenotype against five different antibiotics, and the isolated bacterium was identified by 16s rRNA sequencing. Terminalia bellerica Roxb., Baheda has established antimicrobial activity against Gram-negative and Gram-positive bacteria hence the above plant was selected for the present investigation. Ethanolic extract of Terminalia bellerica outer coat, fruit, and the seed was tested for antimicrobial activity against the isolated Multi Drug-Resistant Bacteria, and the MIC90 value was determined. Ethanolic extract of T. bellerica outer coat having the highest zone of inhibition was further fractionated, and four individual fractions, F1 (water), F2 (50% ethanol and water), F3 (ethanol), and F4 (acetone) were tested for antimicrobial activity against isolated Multi Drug-Resistant Bacteria. Staphylococcus saprophyticus, which was isolated from Hospital effluent and identified by 16 s-rRNA sequencing, was found to be...
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Posted by admin on Sep 30, 2021 in |
Pioglitazone is an oral anti-diabetic agent used in treating class – II diabetes mellitus. T1/2of pioglitazone was 3-6 hrs and is eliminated rapidly. Hence sustained release is needed to prolong its duration of action and to increase its oral bioavailability. The main aim of the study was formulation and evaluation of an in-situ gel system of Pioglitazone to increase its bioavailability as a convenient dosage form. Method of Ion-sensitive in-situ gelation was used in this study. Total 15 formulations were prepared with Guar gum, Xanthan gum, and Carbopol-934 in various combinations and assessed for physical appearance, pH, viscosity, in-vitro gelling capacity, drug content, and in-vitro drug release. FTIR, DSC for Pioglitazone, excipients used, and optimized formulation were conducted. In-vivo drug kinetic studies were conducted for optimized formulation. Formulations showed an optimum viscosity allowing ease of administration and swallowing. All formulations have shown pH between 6.9-7.3, floating lag time was 2-3sec and floated for >12 hrs. The x-ray image studies are also confirming the same thing. In- vitro drug release...
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