Posted by admin on Jul 31, 2021 in |
High-Performance Liquid Chromatography is an important and powerful analytical technique for the identification, quantification and isolation of natural products. In the present investigation, a Reversed-Phase- HPLC method was developed for the identification and quantification of the marker compound quercetin in Euphorbia thymifolia Linn. (whole plant) and Manilkara hexandra Roxb. (leaves). Both the plants have been used to cure various ailments since a long time in India. The results confirmed the presence of quercetin in both the plants, and its concentration was found higher in M. hexandra than E. thymifolia extract. Thus, the study justified the traditional use of these plants as medicines, and both the plants could be used in the formulation of some quality drugs in the future; however, further studies are required in this...
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Posted by admin on Jul 31, 2021 in |
Integration of nano-science in medicine leads to the development of biomedical products that help society in a faster and safer manner. In the present studies, Zinc nanoparticles were synthesized by green synthesis route using aqueous extracts of Banium persicum, and characterization was done through UV, SEM, TEM, FT-IR, and XRD. The synthesized nanoparticle was spherical in shape with an average size of 20 nm. Various bioactive compounds present in aqueous extract of this plant were responsible for bioreduction of nanoparticles. Further, these nanoparticles were tested for various biological activities. In antimicrobial activity maximum, anti-bacterial activity was observed against S. aureus at 120 µg/ml (IZ-18 mm),while in the case of fungus, maximum potential was observed at against C. albigans at 120 µg/ml (IZ- 30 mm). Further nanoparticles were screened for their antioxidant activity by LPO, Peroxidase, and Catalase assay and found they possessed potent antioxidant activity. The platelet aggregation of nanoparticles was assayed by Prothrombin (PT) and Activated Partial Thromboplastin time (APTT). In PT assay, maximum activity was observed at...
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Posted by admin on Jul 31, 2021 in |
The medicinal plant Citrullus colocynthis is very significant for indigenous medicine. The pharmacological effects of root, fruits, seeds, leaves, and the whole C. colocynthis have been practices for the treatment of diseases. Phytochemical screening of the fruits of C. colocynthis has observed several bioactive compounds. The glycosides, flavonoids, alkaloids, carbohydrates, fatty acids, and essential oils contain phenolic and flavonoids in the C. colocynthis fruit. In current research work, a review on pharmacological study of medicinal plant C. colocynthis. Many related research articles were checked and collected in Google Scholar, PubMed, Science direct, the scientific information Database, and Scopus. For the present study purpose, using the terms colocynthis, Indrayan, Garhtumba, and Kharatumba in the title of all articles published to 2021 were observed. This study suggests that the extract of C. colocynthis could offer cheaper herbal drugs. The finding of the present research work would be significant, help prepare less costly, eco-friendly herbal drugs to treat various diseases and infections to replace synthetic...
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Posted by admin on Jul 31, 2021 in |
The plant Scoparia dulcis Linn. belongs to the family Scrophulariaceae, is a native of tropical America, commonly called a sweet broom. The traditional healers identified its uses as anti-diabetic, antipyretic, antihypertensive, diuretic, and they have developed many promising traditional medicines and formulations from this plant. The main objective of this present study reveals about the phytoconstituents present in the leaves extract by preliminary phytochemical study, isolation of active compounds by column chromatography, and characterize the isolated compound by spectral studies like UV, IR, NMR, and MASS spectra. The preliminary phytochemical study reveals that the plant possesses more phytochemicals such as carbohydrates, alkaloids, tannins, phenols, flavonoids, vitamin C, and amino acids. The quantitative estimation shows that it consist of a considerable quantity of flavonoids, Phenols, Vitamin C. The methanol extract was prepared and applied in column chromatography to isolate the phytoconstituents. A dull white crystalline compound was isolated in the fraction of Acetone: Ethanol (60:40), (40:60) ratio. This compound was subjected to spectral studies. The spectral studies indicate the presence...
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Posted by admin on Jul 31, 2021 in |
This paper presents the antimicrobial activity of instant coffee, a prevalent beverage utilized by millions of people worldwide every day. Several studies have shown that instant coffee contains many useful bioactive compounds, the most important: caffeine, trigonelline, chlorogenic acid, ferulic acid, caffeic acid, nicotinic acid, and phenolic compounds which are responsible for the antimicrobial activity. In this study, six strains of resistant bacteria were selected as follows (Methicillin-resistant Staphylococcus aureus MRSA, S. aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumonia, and Acinetobacter baumannii). The antibiotic sensitivity test revealed that the most tested bacterial strain was utterly resistant to the selected antibiotic discs. The antimicrobial activity of instant coffee was evaluated at different concentrations (20, 40, 60, and 80 mg/ml) against tested multi-drug resistant bacteria (MDR) and the fungi (A. niger and A. flavus). The results of antibacterial effect of instant coffee showed that the inhibition zone ranged between (7.6±0.5 and 19.6±0.5 mm). While the minimum inhibitory concentration (MIC) ranged between (62.5 and 32.25 mg/ml) against tested bacteria. Moreover, the instant...
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