Posted by admin on May 31, 2021 in |
Now a day’s interest towards natural and has been growing due to the unhealthy consequences of chemicals in the health industry; though, herbal substances possess several quality control and confirm pharmacological action issues. This present study was designed to determine the effects of Salix alba L. methanolic extract (MESAL) for its antioxidant and anti-inflammatory activity in rat models. Further to establish and validate a sensitive, fast and reproducible high performance thin layer chromatographic (HPTLC) method of two biomarker compounds Salicin and Ferulic acid from MESAL. The anti-inflammatory activity was studied by the carrageenan-induced rat paw oedema method while DPPH free radical scavenging ability was utilized to determine the antioxidant activity. Additionally, the separation was performed by HPTLC with quantification of markers (Salicin and Ferulic acid). Among the different combinations of mobile phases used, the best separation was achieved in Toluene: Ethyl acetate: Methanol: Formic Acid (5:3:1:1v/v/v/v). The MESAL exhibited antioxidant activity with a maximal inhibitory concentration (IC50) value of 400 µg/ml, and exerted anti-inflammatory activity, wherein 70 % protection...
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Posted by admin on May 31, 2021 in |
The present study investigates the anti-parkinsonian activity of various extracts of Justicia gendarussa (JG) leaves in MPTP-induced neurotoxicity mice. Parkinson’s disease (PD) is a neurodegenerative syndrome belongs to a group of motor symptoms disorders. In PD, brain cells degenerated in some parts of the brain which produce dopamine. JG herb is widely distributed in tropical regions of the world. The various parts of the plant are employed traditionally in the treating of various disorders. The present study was designed with 11 group’s mice with 6 each. Group 1 (control), Group 2 (MPTP 20 mg/kg IP four injections at 2 h intervals), Group 3 (L-dopa), and Group 4 to Group11 were administered with Ethanol, Ethyl acetate, Aqueous and n-hexane extracts for 7 days with dose 200 and 400 mg/kg orally along with MPTP 4 injections on day 1. After 7 days, Neurotransmitters, in vivo antioxidant levels, histopathology observation, and isolation of Phytoconstituents by column and characterization studies were performed. The results of the study showed that significant alterations in neurotransmitter...
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Posted by admin on May 31, 2021 in |
Capparis decidua Edgew. (Capparidaceae) is an important medicinal plant widely used in Ayurveda as anti-inflammatory, anti-arthritic, hepatoprotective, diuretic, digestive, laxative, analgesic and anti-asthmatic. In this study, a sensitive and reliable high-performance thin-layer chromatography (HPTLC) method has been developed for the quantification of a flavonol quercetin as an important marker in C. decidua stem. Ethyl acetate extract prepared from hydrolyzed alcoholic extract was applied on precoated silica gel G 60F254 plate and the plate was developed using chloroform-methanol-formic acid (9:1:0.6, v/v/v) as the mobile phase. The detection and quantification was carried out by densitometric scanning at 366 nm. The system was found to provide good resolution of quercetin from other constituents present in C. decidua stem. The method was validated for linearity, inter-day and intra-day precision, repeatability, accuracy, specificity, limit of detection and limit of quantification. The linear regression analysis data for the calibration plots showed a good linear relationship (r2 = 0.995) in the concentration range of 500-1500 ng. The accuracy of the method was confirmed from recovery studies...
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Posted by admin on May 31, 2021 in |
A simple, accurate, and precise reversed-phase high-pressure liquid chromatography (RP-HPLC) method was developed for the simultaneous estimation of nimesulide and dicyclomine hydro-chloride in fixed-dose combination tablets. The developed method was validated as per ICH guidelines Q2(R1). The chromatographic separation was achieved on Hypersil ODS C18 (200 × 4.6 mm 5 μ) column using methanol: phosphate buffer (pH 4) 40:60 (v/v) as the mobile phase at a flow rate of 1 mL/min at 25 °C and detection at 215 nm. The retention times of nimesulide and dicyclominehydrochloride were 3.32 min and 4.22 min, respectively. The linearity range of nimesulide was 2.5-80 μg/mL, and that of dicyclominehydrochloride was 150- 650 μg/mL. The percent recovery of nimesulide and dicyclomine hydrochloride was found to be in the range of 98-102%. The developed method was validated as per ICH guidelines Q2 (R1) and can be used for the estimation of nimesulide and dicyclomine hydrochloride in fixed-dose combination tablets. The developed method can be extended to other dosage forms containing nimesulide and dicyclomine hydrochloride with...
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Posted by admin on May 31, 2021 in |
Medicinal plants, herbs, spices, and herbal remedies are integral components of the alternative system of medicine since while’s ancient Cassia auriculata Linn is a potential folklore medicinal plant (Caesalpiniaceae) used for Ayurveda and Siddha systems of medicine. Plants contain a wide variety of phytochemical constituents, which are secondary metabolites and are used either directly or indirectly in the pharmaceutical industry. For centuries, man has effectively used various components of plants or their extracts for the treatment of many diseases. The aim of the study was to investigate the phytochemical compounds of Cassia auriculata and GC-MS analysis. The presence of phytochemical compounds was screened by a qualitative method using gas chromatography and mass spectroscopy (GC-MS). An aqueous and ethyl acetate extract obtained were subjected to GC-MS for the determination of bioactive volatile compounds. GC-MS analysis was carried out using claries 680 GC with clarus 600 (EI) MS. The GC-MS analysis of the aqueous and ethyl acetate extract revealed the presence of 26 bioactive compounds with valuable biological activities. The major...
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