Posted by admin on Apr 30, 2021 in |
Angiogenesis is an inevitable process of tumour growth, invasion, and metastasis. Understanding the process of new blood vessel formation has gained prime importance recently, and anti-angiogenic therapy has now been considered as an important strategy for cancer therapy. Many bioactive compounds, especially of plant origin, have been explored for their anti-angiogenic potential. The present study was formulated to evaluate the effect of Majorana hortensis leaf extract on angiogenesis, in an effort to examine its influence against cancer cell proliferation, invasion, and metastasis. Cell viability analysis in non-transformed chick embryo fibro-blasts showed that Majorana hortensis leaf extract alone exhibited slight cytotoxicity as assessed by MTT and SRB assay. However, in combination with etoposide, the leaf extract exhibited a synergistic effect as evident from the improved cell viability in chick embryo fibroblasts. Using CAM assay, the anti-angiogenic potential of the Majorana hortensis leaf extract alone was inferred from the significant reduction in blood vessel formation. The anti-invasive potential of M. hortensis leaf extract was further substantiated by a scratch assay using...
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Posted by admin on Apr 30, 2021 in |
The biotransformation of different nitrile compounds into valuable acids was attempted using whole cells of nitrile hydrolyzing enzyme produced from a bacteria strain (BITSN007) isolated from sludge sample of Hot spring Surajkund, Jharkhand. Based on the biochemical characterization, the isolate producing nitrilase is predicted as Bacillus sp. The nitrilase production was observed as intracellular and inducible in nature. The preliminary results showed broad substrate specificity of enzyme, and the highest activity was obtained at 50 ºC and at pH 7.0 with a substrate concentration of 5 mM. Out of all the substrates, nitrilase showed greater activity for propionamide and converted it to propionic acid, which finds application in different food and chemical...
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Posted by admin on Apr 30, 2021 in |
The SARS-CoV-2 virus is the causative agent of the Covid-19 pandemic. The major proteins of this virus, like covid main protease RNA dependent RNA polymerase, are being extensively studied with the hopes of finding an effective molecule that would bind to these proteins and lead to their inhibition. The inhibition of these critical viral proteins would hamper SARS-CoV-2 infection. With most of the currently available drugs falling short of providing an absolute cure and the reported side-effects of the current medications, a lot of phytochemicals are being investigated for their efficacy against the viral proteins. In our preliminary in-silico analysis, we found that the Ocimum Sanctum (Holy Basil/Tulsi) flavonoids, orientin, and vicenin-1, showed energetically favoured docking with the functionally critical amino acid residues of covid main protease, RNA dependent RNA polymerase, Spike protein, nucleocapsid, and 3a proteins of SARS-CoV-2 virus. These results hold promise for orientin and vicenin-1 to be further investigated as potential therapeutic drugs against SARS-CoV-2...
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Posted by admin on Apr 30, 2021 in |
The aim of this research was to examine the protective effect of D-Pinitol on haematological profile against Doxorubicin-induced cytotoxicityin mice. Doxorubicin is a chemotherapeutic agent valuable in treating various cancers regardless of its toxicity to normal cells, including hematopoietic suppression. Oxidative stress and generation of reactive oxygen species appear to be the reason for Doxorubicin-induced cytotoxicity in bone marrow cells. D-Pinitol is a richly available carbohydrate in Soybean plants and it has been shown to possess free radical scavenging activity and antioxidant effect in previous researches. Sixty Swiss Albino mice were divided into ten groups: Control, Doxorubicin (5 mg/kg), D-Pinitol (100 mg/kg, 200 mg/kg, 300 mg/kg and 400 mg/kg) and D-Pinitol (100 mg/kg, 200 mg/kg, 300 mg/kg and 400 mg/kg) + Doxorubicin (5 mg/kg). Blood samples were taken from the mice to determine the blood levels of haematological parameters such as white blood cells, lymphocytes, monocytes, red blood cells, hemoglobin, hematocrit and platelets. The D-Pinitol-only treated groups reported no significant variations in haematological parameters compared to the control group....
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Posted by admin on Apr 30, 2021 in |
Aim: To estimate the impact of clinical pharmacist mediated patient counseling in dyslipidemia patients in RIMS hospital, Kadapa. Objectives: To conduct a randomized study on patients. To categorize patients on demographic basis, comorbidities. To compare the lipid levels based on 20 mg and 40 mg doses of atorvastatin, the outcome based on lipid profile. Methodology: It is a prospective observational, comparative type of study. Conducted in the department of general medicine, Rajiv Gandhi institute of medical sciences at Kadapa with a period of Six months (July 2018 to December 2018), and the sample size was 72 subjects. Results: A total of 72 patients were included in the study 86.11% were male, and 13.88% were female. Average age was found to be 57.11 years. Average BMI of males is 25.4 and females is 27.17 .36 patients included in group A (statin therapy) and 36 patients included in group B (statin + patient counseling). The P value of lipid levels in group A, and group B with two different doses of...
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