Posted by admin on Dec 31, 2021 in |
Oral administration of 131I to the patients of differentiated thyroid cancer is routinely used to ablate remnant thyroid tissue or metastasis. In 30% of the patients, permanent salivary gland damage due to therapeutic 131I exposure results in xerostomia, affecting their quality of life. Amifostine is the only standard FDA-approved radio protect ant available for damage control, albeit associated with side effects such as hypotension and allergic reactions. The present study was carried out to compare the radioprotective effect of O. sanctum extract with that of a standard radio protectant, amifostine in salivary glands of the rabbits exposed to high dose (1GBq) of 131I internal radiation exposure. The study parameters included were salivary amylase, serum SGOT and SGPT, haematological parameters, 99m Technetium pertechnetate scintigraphy study, histopathology and ultrastructure of the salivary gland. The experimental rabbits were sacrificed after 6 months of 131I exposure. The experimental observations, majorly histopathological and electron microscopic are suggestive of better preservation of cell morphology and ultrastructure after O. santum as well as amifostine pre supplementation....
Read More
Posted by admin on Dec 31, 2021 in |
Urena lobata is used by traditional practitioners for treating external wounds. A preclinical study was conducted to evaluate the efficacy of the ointment formulations containing ethanolic extract of Urena lobata (EO) (5%) and nanosized extract (NO) of U. lobata (5%) for their wound healing activity by assessing wound contraction, tensile strength, superoxide dismutase, catalase, reduced glutathione, myeloperoxidase, hydroxyproline, lipid peroxidation and his to pathological studies in experimentally-induced open wounds in Swiss albino rats. Quercetin, an active principle of the formulation, was estimated qualitatively and quantitatively through HPLC studies. Topical application of NO and EO formulations promoted faster reepithelialization, wound contraction and maximized tensile strength, increased SOD, catalase activities and GSH, hydroxyproline levels with lower myeloperoxidase and lipid peroxidation in comparison with control. Histopathological studies revealed lesser inflammatory cell proliferation, degeneration, with marked fibroblast proliferation and collagen maturation than the control group. Results indicate that both NO and EO formulations showed benefits in the management of wounds when compared to the standard povidone...
Read More
Posted by admin on Dec 31, 2021 in |
Discovery of the drug molecule which has therapeutic values is the backbone of drug development. Pharmaceuticals have an important role in the prevention and treatment of diseases in humans and animals. However, gradual change in the process of drug synthesis from drug development to bulk production is a common complication in the drug development process. This change may result in the formation of impurities. Moreover, impurities can also be formed during transport and storage. The presence of more than the prescribed limit of impurities in the drugs may influence the purpose of the pharmaceuticals. Therefore, the evaluation of pharmaceutical drugs for the identification and quantification of impurities at various stages is essential. Several chemical and instrumental methods are used to assess the impurities. For this purpose, analytical instrumentations and methods play a crucial role. The current review highlights the development of impurities at various stages of drug development and pharmaceutical manufacturing. Moreover, this review put light on the recent advancement in the analysis of...
Read More
Posted by admin on Dec 31, 2021 in |
Fostemsavir was quantified using a simple and reliable HPLC technique developed and validated. PhenomenexC18 column was used to achieve chromatographic segregation (150 x 4.6 mm, 5m). The mobile phase was a 55:45 mixture of 0.01N disodium orthophosphate and acetonitrile pumped through the column at 1.0ml/min. The temperature was maintained at 30 °C and the buffer used was 0.01N sodium dihydrogen phosphate. The wavelength of the ideal choice was 278.0nm. Fostemsavir’s retention time was discovered to be 2.471 minutes. Fostemsavir’s percent RSD was found to be 0.7 percent. For Fostemsavir, the percentage recovery was 100.07 percent. Fostemsavir’s regression equation yielded LOD and LOQ values of 0.18 and 0.54, respectively. Fostemsavir’s regression equation is y = 26381x + 8398.8. Since run time and retention time were minimized, the system developed was simple and cost-effective, and it could be used on a daily basis for quality...
Read More
Posted by admin on Dec 31, 2021 in |
To study the hepatoprotective activity of ethanolic leaves extracts of Abroma augusta against non-alcoholic fatty liver disease to validate ethnobotanical claim regarding the plant used in the above mentioned disease. Materials and Methods: The hepatoprotective potential of Abroma augusta leaves extract (AALE) (250 and 500 mg/kg/ body weight) was studied on Methionine and Choline deficient diet (MCD diet), High Fat Diet (HFD), Cholesterol and Cholate diet (CCD) and streptozotocin + HFD induced non-alcoholic fatty liver disease. In the last treatment, blood was collected from direct cardiac puncture and analyzed for various parameters like alanine aminotransferase (ALT), aspartate transaminase (AST), triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), free fatty acid (FFA) and malondialdehyde (MDA). Results: The present study showed for the first time that AALE reduced level of ALT, AST, TG, TC LDL, FFA, MDA and possessed hepatoprotective activity as evidenced by its significant inhibition in the formation of hepatoxicity induced by diet and chemical agents with maximum curation (500mg/kg/b.w.) against non-alcoholic fatty liver disease. It...
Read More
