Posted by admin on Jun 30, 2022 in |
Objective: A simple, rapid, accurate, precise and reproducible validated RP- HPLC method was developed for the determination of Lecithin and Silymarin in bulk and tablet dosage forms. Methods: A simple double beam UV spectrophotometric method has been developed and validated with different parameters such as linearity, precision, repeatability, the limit of detection (LOD), Limit of Quantification (LOQ), accuracy as per ICH guidelines. Results: The quantification was carried out using Symmetry PremsilC18 (250mm x 4.6mm, 5µm) column run in an isocratic way using mobile phase comprising of acetonitrile: water (0.1% OPA with PH 3) 70:30 v/v and a detection wavelength of 236 nm and injection volume of 20µL, with a flow rate of 1ml/min. The retention times of Lecithin and Silymarin were found to be 6.58 min and 3.616min. The method was validated in terms of linearity, precision, accuracy, LOD, LOQ, and robustness in accordance with ICH guidelines. The assay of the proposed method was found to be 99-101%. The recovery studies were also carried out, and the mean %...
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Posted by admin on Jun 30, 2022 in |
Polysaccharides are composed of monosaccharide’s linked by glycosidic bonds. These are abundantly available and are obtained from renewable resources. Polysaccharides provide an array of primary structures and conformations used in the pharmaceutical industry. Natural polysaccharides properties can be substantially modulated by chemical, physical, and enzymatic modifications and have received increased attention to expanding the landscape of application of polysaccharides in diverse sectors. Modifying the natural polysaccharides properties and structures gives functionally improved polysaccharides with excellent bioavailability, biocompatibility, structural stability, and versatile chemical. By modifying the physicochemical properties of polysaccharides, systems like micelles, hydrogels, liposomes, niosomes and vesicles are produced. The modified polysaccharides have a remarkable application in gene delivery, targeted cancer therapy, delivery of the drug, wound dressings, tissue engineering, nanocarriers, biosensors, pharmaceutical formulations, agricultural applications, and food industry. This article covers different types of polysaccharides and distinct methodologies applied during the new modified synthesis of polysaccharides and also the study of their properties after modification. This review also highlights the application of these modified polysaccharides in various pharmaceutical,...
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Posted by admin on Jun 30, 2022 in |
To compare the effect of combined oral contraceptive (COC) and progesterone-only injectable contraceptive (PIC) on the inflammation, we recruited 47 female participants (16 to 43 years old) in 3 groups: control participants (n=10), COC users (n=16) and PIC users (n=21). Using ELISA and Latex agglutination methods, we measured the effect of hormonal contraceptives on the inflammatory blood biomarker, C-reactive protein (CRP). Data were collected from July 2019 to January 2020 from Rajshahi Medical College and Hospital. The users of both COC and PIC had higher levels of CRP (p<0.001), as compared to the controls, and COC users had higher levels as compared to PIC users (p<0.001). We also found a correlation between the CRP data of contraceptive users and some socio-demographic variables. The CRP values of different demographic variables in the Elisa method but not in the Latex method were significantly correlated (p<0.01). There was a significant association between the types of contraceptives used, as measured both Latex and Elisa methods. Our data suggested that limiting hormonal contraceptives can...
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Posted by admin on Jun 30, 2022 in |
Adenosine release is more apparent after seizures or in-vitro seizure-like activity and tissue adenosine level increases quickly after seizure commencement. These adenosine increases linked to seizures are reflections in composing a response mechanism that controls seizure intensity and duration. Much evidence from animal studies suggests that this impact is mediated by the adenosine receptor (AR), just as it is with neurotransmission in general. Efforts to produce centrally acting treatments and reduce peripheral side effects, especially cardiac adverse effects, have long been prioritized in drug development. New study is targeting highly precise areas of the A2R might be a step forward in direct A2R-based anti-seizure therapy. Despite significant attempts to introduce innovative tactics for the treatment of various kinds of epilepsy, this illness continues to be a serious problem across the globe. Taking inspiration from the facts above and recognizing the growing need for anti-epileptic compounds with improved pharmacodynamics and pharmacokinetic profiles in the worldwide market. The present in-silico research involves studying the anti-convulsant (anti-epileptic) potentials of some substituted 2′-(prop-1-en-2-yl)-1′,2′,3′,4′-tetrahydro-[1,1′-biphenyl]-2,6-diol...
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Posted by admin on Jun 30, 2022 in |
ACAT enzyme is found in human body and it maintains cholesterol homeostasis in the cell. This leads to deposition of cholesterol in our body as cholesteryl esters. Freely deposited cholesterol in the membranes of the cells is cytotoxic. This deposition causes atherosclerosis, Alzheimer’s disease, Xanthelasma, etc. The first substrate for ACAT is both medium & long fatty acyl CoA. Therefore, we studied the ACAT enzyme, its active site, location and activity to develop the inhibitors of ACAT as antihyperlipidemic agents with the heterocyclic compounds for drug designing. ACAT is having two active sites: histidine and aspargines. ACAT 1 is present in two chromosomes 1 and 7. Active site His-460 Of ACAT-I is responsible for catalysis. For docking studies these factors should be known so that the hetercyclic compound could be developed having inhibitory activity against ACAT. Further many literature review have been done to find a hetercyclic moiety having the antihyperlipidemic activity in which we found that benzene ring with thiazole and oxazole rings having side chain could get...
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