Posted by admin on May 31, 2022 in |
Objective: Active chemical constituent in plants exhibits pharmacological action. Hence this research work aimed to identify and quantitate oleanolic acid. Methods: A simple, high-performance thin-layer chromatography (HPTLC) is developed and validated to identify and quantify oleanolic acid. The extracts were spotted as bands on aluminum TLC plates which were pre-coated with silica gel. Different compositions of mobile phase tried for high resolution and reproducible results. Mobile phase composition toluene: methanol: ethyl acetate (8.5:1:0.5 V/V) utilized. Plates were derivatized with 10% sulphuric acid and scanned. Oleanolic acid was detected quantitatively at 529nm. Result: In the selected mobile phase, Rf value for oleanolic acid was found to be 0.35, which indicated a good separation. The Chromatography method was validated with respect to Linearity range (ng), Intraday and Interday precision, Instrumental Precision, Quantitation Limit i.e., LOQ (ng), Detection Limit, i.e., LOD (ng), Repeatability for oleanolic acid as per ICH guidelines. Oleanolic acid calibration graph in 200-1200 ng range per band was found linear with correlation coefficient value 0.998. All the parameters obeyed...
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Posted by admin on May 31, 2022 in |
Determine the fungicidal activity of five disinfectants on Candida auris of clinical and environmental origin. Assess the fungicidal efficiency of each disinfectant on the microorganism. Calculate the percentage of efficiency (% E) of each disinfectants on Candida strain and determine the specific death rate (k) and decimal reduction time for microorganisms. Analytic and experimental research with quantitative methods. We realized a strain’s workbench by culturing it in corresponding culture media. Analysis was a challenge essay, performed on four surfaces and at different exposure times; 1, 5, 15, and 30 minutes of contact between disinfectant and yeast. Once the disinfection process was carried out, what was expected was that after 15 minutes of contact between the yeasts and the disinfectants, a percentage of inhibition of the microorganism between 90 and 95% was presented, thus guaranteeing the duration of elimination of the infectious agent by disinfectants. Results show that after one minute of contact between Candida auris and disinfectant in a gloved hand, the yeast was removed when the clinical and...
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Posted by admin on May 31, 2022 in |
Background & objectives: Diallyl disulphide (DADS) is an active component of garlic that has been claimed to have hypoglycemic effect and are beneficial in achieving glycemic control in diabetes mellitus (DM). The present study was conducted to assess the glucose utilization in erythrocytes in diabetic subjects after the exposure of DADS. Methods: The blood samples were collected from diabetic subjects; the plasma and erythrocytes were separated. The fasting plasma glucose levels were measured and the separated erythrocytes from diabetic blood samples were preincubated and posted incubated with DADS. The glucose utilized by erythrocytes and the percentage of glucose utilization were measured. Results: The present study results showed that diabetic erythrocytes exposed to DADS showed a significant rise of glucose utilization compared to untreated erythrocytes, which signifies the positive influence of DADS on glucose utilization in diabetic patients’ erythrocytes. Conclusion: DADS, through the formation of thiol derivatives, promotes glucose utilization by mimicking the actions of insulin in diabetic erythrocytes after post-incubation. This study can be concluded that DADS can be...
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Posted by admin on May 31, 2022 in |
The plant Salvia hispanica belongs to the family Lamiaceae, a highly valued medicinally important seed, commonly known as chia seeds. About 75% of the fats in chia seeds consist of omega-3 alpha-linolenic acid (ALA), while about 20% consist of omega-6 fatty acid. Omega-3s play an important role in sharpening memory and improving mood. Chia seeds also contain polyunsaturated fatty acids, dietary fiber, proteins, vitamins (A, C, E, niacin, thiamine), and minerals. Besides this, the seeds are an excellent source of polyphenols and antioxidants, such as caffeic acid, rosmarinic acid, myricetin, quercetin, and others. These antioxidants exhibit the capacity to scavenge free radicals. Chia’s health benefits as a dietary supplement and efforts are underway to promote it as ‘functional food’ owing to the presence of α-linolenic acid, an essential precursor fatty acid of physiologically significant polyunsaturated fatty acids. Chia seeds also possess various pharmacological activities like anti-inflammatory, antihypertensive, anti-diabetic, anti-cancer and it also reduces the risk factors of heart disease. It is commonly used for weight loss. And it also...
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Posted by admin on May 31, 2022 in |
Anorectal disorders are often perceived in surgery practice and often reside with pain with shifting vigour. Endeavour has been made to lessen pain and avoid ligature. Minuscule hospital stay with early return to work is the main aim of developing newer techniques in the management of anorectal diseases. Diverse modalities with the main tensity on surgical interceding for managing more common anorectal disorders are...
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