Posted by admin on Feb 28, 2022 in |
Oral cancer is the sixth leading cancer globally and the leading carcinoma in the Indian subcontinent. The relatively high incidence is due to usage of tobacco, and exposure to carcinogens are frontrunners of oxidative stress-induced DNA damage. The practice of chemopreventive agents is considered to be alternative oral carcinoma. A study on novel natural chemo preventive agent-Indigofera Aspalathoides was undertaken to evaluate its preventive chemo effect. Male Syrian Golden hamsters aged 10-12 weeks and weighing about 80-100 g were selected. Group 1were control; Group II hamsters were given Intragastric administration of ethanolic fraction of IA; Groups III had Intragastric administration of chloroform fraction of IA, Group IV had Intragastric administration of E IA + DMBA ,Group V had DMBA + Intragastric administration of CIA, and Group VI with DMBA alone. Total antioxidant power and extend of lipid peroxidation was measured by gold standard methods. Our study suggests that both EIA and CIA had a significant anticancer and preventive chemo effect against squamous cell carcinoma. Elevation in the extent of...
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Posted by admin on Feb 28, 2022 in |
Curcumin is used as a potent anti-inflammatory agent in the treatment of rheumatoid arthritis and its clinical pharmacokinetics requires a sensitive method for estimation of its plasma concentration. Hence the aim of the study was to develop a rapid, selective, and sensitive HPLC method coupled with UV detection for the determination of Curcumin in rat plasma. The process of elution was carried out on Phenomenex Luna C18 (250*4.6 mm id, 5 μm particle size, 130 Å) column using acetonitrile and 1 % formic acid mixture in the ratio of 70:30 % v/v as mobile phase delivered with a flow rate of 1.0 mL/min. Detection was carried out using a UV detector at 420 nm. The method was found to be linear in the range of 100 to 3000 ng/ml with R2 close to one (0.9969). The limit of detection (LOD) and limit of quantification (LOQ) of CUR was found to be 2.82 ng/mL and 8.5 ng/mL, respectively. The method was validated for accuracy, precision, linearity, LOD, LOQ, and robustness....
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Posted by admin on Feb 28, 2022 in |
The work aimed to prepare super porous hydrogels (SPHs) of Amoxicillin trihydrate to release the drug in a sustained manner in the gastric environment and study the polymeric ratio and pore effect formers on the physicomechanical properties and drug release kinetics of the formulations. Fourier Transform Infra-Red (FTIR) studies were done to check the compatibility of the drug with the polymers. The super porous hydrogel formulations were prepared in two batches (A and B) using sodium chloride and sodium bicarbonate as pore formers, respectively. Scanning Electron Microscopy observed the structural morphology of hydrogel. The presence of a large number of pores with sizes ranging from 1 to 10 μm was revealed, confirming the formation of a super porous hydrogel. As dictated by in-vitro dissolution data, the increases in the polymer concentration of HPMC K4M progressively retards and prolongs the drug release; however, no significant change in the effect was observed among NaCl and NaHCO3 pore formers in the super porous hydrogel. The drug release data were fitted into different...
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Posted by admin on Feb 28, 2022 in |
Standardization of herbal formulation is essential to assess the quality of drugs for its therapeutic efficacy. Pancha Haritha kadi Churna (PHC) is a traditional poly herbal formulation meant for digestion which consists of five ingredients having digestive property. It is used for the treatment of Constipation and Bloating. Churna’s will play an important role in gastro intestinal problems and they are having greater bioavailability because of smaller particle size. It consists of fine powder (sieve 100 size) of Ginger rhizomes, Fennel fruits, Myrobalan fruits, Senna leaflets and Pink Rock salt in equal proportions (1:1:1:1:1) are mixed well. PHC is formulated by standard procedures and evaluated by physical parameters, phytochemical analysis and HPTLC studies, the values are compared with that of marketed churna. Physical parameters were found to be within the standards, phytochemical analysis shows the presence of Alkaloids, Terpenoids, Steroids, Tannins, Saponins, Coumarins, Quinones, Flavones, Flavanones, Phenols and Carbohydrates whereas in HPTLC fingerprinting profile, seven peaks of PHC were matching with marketed...
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Posted by admin on Jan 31, 2022 in |
A simple, precise, rapid, linear and economical RP-HPLC method was developed and validated for simultaneous estimation of Pregabalin and Etoricoxib in pharmaceutical tablet dosage form. The determination was performed on Phenomenex C18 (250 mm × 4.6 mm, ID, 5µ) column at 30 ºC temperature using a mobile phase Acetonitrile: Water (55:45 %v/v) at a flow rate of 0.8 ml/min was employed. The RP-HPLC detection wavelength was 200 nm and 235 nm for Pregabalin and Etoricoxib, respectively. This method obeys Beers law in employed concentration range 2.4-12 µg/ml and 0.8-4 µg/ml for Pregabalin and Etoricoxib, respectively. The correlation coefficient of Pregabalin and Etoricoxib was found to be 0.9993 and 0.9997, respectively. The retention time was 2.680 and 7.383 minutes. The percentage RSD for accuracy and precision was found to be less than 2%. The method was validated as per ICH guidelines for its selectivity, specificity, system suitability, linearity, range, precision, accuracy, LOD, LOQ, robustness, assay. The method was successfully employed for routine quality control analysis of Pregabalin and Etoricoxib in...
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