Posted by admin on Nov 30, 2023 in |
Autism spectrum disorders (ASDs) refer to the group of neuropsychiatric disorders that alter neurodevelopment and mental function and are characterised by an extensive range of symptoms, including hyper-or hyposensitivity, repetitive behaviours, and impairments in social and academic functioning. Untangling the causation of ASD has been complicated, in part because distinct genetic abnormalities can result in both identical and distinctive traits that entirely fall within ASD. ASD can be triggered by hereditary and/or environmental causes. Studies in both experimental and clinical trials have revealed that the gut microbiome is altered in ASD patients. Due to its linkages to nutrition and the reciprocal relationship with the host, these changes affect the composition of the gut microbiota and also the metabolites they create. The gut-brain axis (GBA) mediates how the central nervous system (CNS) associated roles and behaviours are affected by psychobiotics, a unique family of probiotics that enhances not only gastrointestinal (GI) function but also anxiolytic and antidepressant activity. Psychobiotic use can enhance GI processes and ASD-related symptoms. New models for studying ASD...
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Posted by admin on Nov 30, 2023 in |
Lysozyme amyloid formation is studied as model in-vitro system to find out natural or synthetic inhibitors of amyloidosis. Protein aggregation and amyloid formation is the basic reason for many neurodegenerative diseases. Indigenous medicinal plants are great source of therapeutic molecules and drugs. Ethnobotanical studies have indicated many plants have neuroprotective functions. We have studied three indigenous medicinal plant namely Bacopa monnieri (BM), Trigonella foenum-graecum (TFG) and Tridax procumbens (TP) showing various pharmacological properties as inhibitors of lysozyme aggregation. We isolated leaf proteins and did the protein aggregation studies. Anti-aggregation properties of leaf extract were carried out by following assays – DTT induced lysozyme aggregation assay, Congo red binding Assay, Thiaflavin binding assay and protein stability assay by Tryptophan Fluorescence. BM leaf protein extract showed maximum inhibition followed by Methi (TFG) and then...
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Posted by admin on Nov 30, 2023 in |
A simple, precise, accurate, and rapid high performance thin layer chromatographic method has been developed and completely validated for the estimation of hydroxychavicol isolated from piper betel leaves powder. The isolation of hydroxychavicol was carried out using flash chromatography. Validation was carried out with pre-coated silica gel aluminum plate G 60 F254 (10 x 10 cm) as the stationary phase, mobile phase consisting of Butanol: Ethyl Acetate in the composition of 7:3 (v/v) and scanned in Absorbance Reflectance mode at 280 nm using Camag TLC scanner 3 with WinCAT software. The Rf value of 0.64 was obtained for isolated hydroxychavicol. The proposed method was validated over a linearity range of 200–800 ng/spot, and its percentage recovery was calculated along with intraday and interday precision. The limit of detection and the limit of quantification were found to be 13.890 ng/spot and 40.457 ng/spot, respectively. The suggested method can be successfully used to detect and quantify hydroxychavicol in herbal...
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Posted by admin on Oct 31, 2023 in |
A sensitive, rapid, precise and accurate RP-HPLC method was developed for simultaneous estimation of Pregabalin and Duloxetine in synthetic mixture. To introduce a chromophoric group in Pregabalin structure, derivatization was carried out through benzoylation reaction. RP- HPLC method is carried out using Hypersil BDS column C18 (250 cm × 4.6 cm, 5 μm). Mobile phase used as combination of Methanol: Water (75:25 v/v) and detection was carried out at 235 nm & 224 nm respectively for Duloxetine and Pregabalin. Retention time of Duloxetine and Pregabalin were found to be 2.15 min and 5.23 min, respectively. The developed method was validated in pursuance of ICH Q2 (R1) guidelines. Linearity range of Duloxetine is 50-100 μg/mL and for Pregabalin is 100-200 μg/mL. Correlation coefficient for both drugs was 0.999 and mean recoveries for both drugs were between 99% – 100%, minimum values of %RSD indicate the accuracy of the method. The detailed quantitative results show that this method is sensitive, precise as well as cost...
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Posted by admin on Oct 31, 2023 in |
A known case of a cervical polyp of a 26-year-old woman presented in the Outdoor-patient Department of Dr. D. Y. Patil Homoeopathic Medical College and Research Centre with complaints of abnormal bleeding in between periods, pain after coition, and profuse white discharge. USG reports confirmed the diagnosis of the cervical polyp. After a detailed history taking of the patient and considering a complete totality of symptoms, Natrum Muriaticum 30C, a single individualized homeopathic medicine, was prescribed to the patient, and it proved effective in the treatment of cervical polyp. The patient slowly showed improvement in physical symptoms, followed by mental symptoms. Modified Naranjo Criteria were used, and regular follow-ups were taken to assess the case. This case demonstrates the usefulness of homeopathic medicines in psychosomatic diseases wherein the psychological cause is the main cause of the disease in the patient. Also, this case shows the effectiveness of Homoeopathic remedies in the treatment of cervical polyps. It can be looked at as an alternative method of treatment with further research...
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