Posted by admin on Sep 30, 2023 in |
The drugs Aceclofenac (ACF), Paracetamol (PAR), and Tramadol Hydrochloride (TH) are analgesics, and the combination is used in the symptomatic treatment of moderate to severe pain. The reported HPLC methods for this combination use complicated mobile phases; none is a stability-indicating method. The present study aims to develop and validate a simple stability-indicating RP-HPLC method for simultaneous estimating ACF, PAR, and TH in bulk and tablet dosage form. The chromatographic separation was performed using Neosphere C18 (250 x 4.6 mm, 5mm) with a mobile phase comprising methanol: 0.5% Triethanolamine. 0.5% Triethanolamine was added to methanol to reduce tailing. The flow rate was set to 1.5ml/min and UV detection was carried out at 270 nm with a run time of 4 min. It was found to be linear in the range of 15-40 µg/ml, 39.9- 106.4 µg/ml, and 129.9-346.4 µg/ml with a R2 value of 0.999, 0.999, and 0.995 for TH, ACF and PAR respectively. Statistical analysis proved that the developed method is simple, accurate, precise, reproducible, specific, sensitive and cost-effective....
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Posted by admin on Sep 30, 2023 in |
The medication pretomanid effectively treats drug-resistant tuberculosis (MDR TB). For the analysis of pretomanid, a simple, focused, and accurate HPTLC method has been created and approved. The samples were applied as bands on a silica gel-coated TLC plate made of aluminium. Pretomanid was completely separated using ethyl acetate and n-hexane as the mobile phase; the RF value was 0.68 0.046. Densitometry was used for detection, namely in absorbance mode at 330 nm. The peaks produced by this approach were discovered to be crisp, symmetrical, and highly resolved. A linear relationship was found across the concentration range of 200-1200ng/spot with coefficients of determination of 0.987. According to the International Conference on Harmonization’s criteria, the method’s accuracy, recovery, repeatability, and robustness were all confirmed. The limit of quantitation was determined to be 439.36 ng/spot, while the lowest detectable level was found to be 144.9 ng/spot. According to statistical analysis of the data, the procedure is exact, accurate, repeatable, and selective for the examination of Pretomanid. The suggested single approach permitted analysis...
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Posted by admin on Sep 30, 2023 in |
Carbamazepine (CBZ) has long been a therapeutic option for bipolar disorder. Carbamazepine is used to treat acute manic and mixed episodes associated with bipolar I disorder. In the present study, bilayer tablet was developed to improve dosing convenience and decrease daily fluctuations in serum CBZ concentration, thereby lowering the incidence of adverse events. In bilayer tablet one layer provides the loading dose by immediate drug release and another layer provides the maintenance dose up to 12 hrs by sustained release. The drug excipient compatibility study was carried out by FTIR and DSC techniques; there was no interaction found. Cross povidone was used as a superdisintegrant in the immediate release layer and controlled release fraction was formulated by using HPMC K 100 polymer. The prepared granules were evaluated for angle of repose, bulk density, tapped density, and compressibility index which showed satisfactory results. The prepared bilayer tablets were evaluated for thickness, hardness, friability and in-vitro release studies. In-vitro dissolution study was carried out for 12 hours using USP dissolution apparatus...
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Posted by admin on Sep 30, 2023 in |
Background: Traumatic brain injury has significant morbidity and mortality. Several prognostic markers have been evaluated to predict outcomes. ABG (arterial blood gas) analyzers are routinely available and predict the value of calcium, lactate and other electrolytes. We evaluated serum calcium and lactate as a prognostic markers in severe TBI. Methods: We prospectively studied 100 patients with severe TBI. Blood samples for calcium and lactate were taken following admission, day 3 and day 7 after trauma and the relationship with mortality and functional outcome was studied. The Glasgow Outcome Scale (GOS) was used to access functional outcomes. GOS≤3 was considered a poor outcome (Group I). GOS>3 was taken as a good outcome (Group II). Results: Mean age was 51 years. Most patients were males, 77% in Group I and 70% in Group II. Patients in the GOS≤3 group were significantly older than patients with a GOS score >3(p=0.007). Lactate levels were significantly higher in the GOS≤3 group on day 3 (p<0.0001) but no association on day 7. GOS and ionized...
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Posted by admin on Sep 30, 2023 in |
Nanoparticles are the modern-day drug delivery system for biocompatible drugs. Numerous products have developed from nanotechnology to develop effective wound healing and inflammatory treatments. Plant extracts (phytoconstituents) have a lot of therapeutically potential because of their distinctive properties, including anti-inflammatory, antioxidant and insulin-sensitizing properties etc. Furthermore, the nanostructure of herbal extracts and phytoconstituents could enhance the bioavailability, influence relieves of the manner of drug carrier systems to the wounded area, and increase permeation ability to the underlying layers of the skin, all of which are essential for the recovery process and treating inflammation. This review emphasizes nano-formulations of plant extracts. Many approaches have already been published for the preparation of metal nanoparticles, including laser ablation, evaporation-condensation, thermal-decomposition, chemical reduction, photochemical processes, and biological methods (Green synthesis of metal nanoparticles utilizing diverse herbal extracts). Some herbal extracts and their phytochemicals are classified as a potential substitute for wound healing and treating inflammation intermediates due to the presence of various bioactive ingredients, the ease of access, and the reduction of adverse effects. Overall, when employed in nano-formulations, various...
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