Posted by admin on Aug 31, 2023 in |
Objective: The study aims to evaluate the granuloma and associated inflammatory mediators inhibition potential of the chloroform extract of Piper pedicellatum C. DC. Leaves, as plant leaves, are being used traditionally in the mitigation of various inflammatory conditions; however, there is an insufficiency of scientific evidence about the relevant bioactivity of the plant. Method: The cotton pellet induced-granuloma model in rats is used in the study, providing insights into subsequent phases of inflammation. Piper pedicellatum C. DC. Leaf chloroform extract at 200 mg/kg and 400 mg/kg and indomethacin (reference standard, 10 mg/kg) were used during treatment. Results: The chloroform extract at 200 mg/kg and 400 mg/kg dose inhibited exudate formation by 25.23% and 38.43%, respectively, and inhibition of granuloma tissue was found to be 27.62% and 40.33%, respectively. Further significant decrease in serum inflammatory markers CRP and PGE2 and reduced cytokine TNF-α and IL-6 were observed in chloroform extract and indomethacin-treated rats. In contrast, a substantial increase in anti-inflammatory cytokine serum IL-10 levels was noted in treated rats. Conclusion:...
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Posted by admin on Aug 31, 2023 in |
An overview of the neurodevelopmental disorder ADHD (attention deficit hyperactivity disorder), its consequences, and details on the brain regions impacted by ADHD are the driving forces behind this review. The hereditary basis of ADHD has been investigated. Throughout the investigation, numerous reviews and research publications were compared and examined. The themes covered in this article are (a) ADHD as well as its symptoms (b) the complexity of the brain regions affected in ADHD patients (c) and (d) linkage analysis for researching the genes of ADHD. The brain’s structure, development, and functioning will alter, according to review analysis results. The main brain areas involved include the cerebellum, parietal, Prefrontal and frontal lobes. According to the study, the thickness of the cerebellum will diminish, and the anterior striatal region will be most negatively impacted. There isn’t much proof that ADHD is inherited, although a few experts have suggested that there may be a connection between siblings’ ADHD and their DNA. For a complete understanding of the condition, a great deal more...
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Posted by admin on Aug 31, 2023 in |
Rosuvastatin is a widely used lipid-lowering medication that belongs to the class of “Statins” whereas Teneligiptin is a novel potent drug that belongs to the class of “DPP-4 inhibitors” which is used for the treatment of Type 2 Diabetes mellitus. The FDA recently approved the combination of these two drugs in 2021 since it proved to be highly effective for treating dyslipidemia associated with Type 2 Diabetes mellitus. Forced degradation studies have been performed on Rosuvastatin and Teneligliptin separately using various analytical techniques such as RP-HPLC, UV, RP-UFLC, RP-UPLC, UHPLC, HPTLC and TLC. Characterization of the degradation products generated during various stress conditions is also performed using various sophisticated techniques such as UPLC, LC/MS/MS, NMR, and FT-IR. These degradants can cause many adverse effects, such as carcinogenicity, mutagenicity, neurotoxicity, hepatotoxicity, skin sensitization, respiratory sensitization, etc. So, identifying and characterizing these degradation products will be very useful for their metabolic studies and in-silico toxicity assessment. In-silico toxicity studies are very helpful in predicting the toxic potential of these degradants, and...
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Posted by admin on Aug 31, 2023 in |
Tuberculosis (TB) is caused by a bacterium called Mycobacterium tuberculosis, identified as a global health emergency by the world health organization. The development of drug-resistance stains due to spontaneous gene mutation has been a major boost to research in the pathogenicity and biochemistry of Mtb. To combat drug resistance to tuberculosis, new drugs and methodologies are emerging. Since, starting itself mycobacterium complex cell wall has been a choice for widely selected targets for anti-TB drugs. Peptidoglycan, arabinogalactan, and mycolic acid are the basic layers supporting cell growth. The current work investigates virtual screening for optimal small molecule inhibitors targeted against selected mycolic acid targets (β-keto acyl ACP synthase, enoyl acyl ACP-reductase and Polyketide synthase 13). A small library of 485 compounds was designed and docked into a selected target core to identify the potential inhibitor. The designed compounds were subjected to docking studies using Glide (Schrodinger). InhA was the most suitable mycolic acid inhibitor target for the designed compounds. Further, the effectiveness of the study was evaluated by comparing the...
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Posted by admin on Aug 31, 2023 in |
Pan-genome analysis can identify the core genome, which is the total number of genes present in all strains of a bacterial species. Further essential genes can be identified in this core set of genes which gives us a minimal number of genes required for the survival of the bacteria. This set of genes can be analyzed to develop new antimicrobial agents against pathogenic, multidrug-resistant bacteria such as Listeria monocytogenes which causes several primary and secondary infections in humans. Pan-genome analysis of 38 strains of Listeria monocytogenes was performed to estimate 2331 genes in core genome, 1056 genes in the dispensable genome, and 152 genes in the strain-specific genome. Essentiality analysis of 2331 core genome proteins predicted 965 essential core-genome families (ECGFs). Furthermore, the identification of host non-homologous proteins using BLASTP with the Homo sapiens proteome resulted in 485 non-homologous essential core-genome families (NH-ECGFs). These putative proteins can be analyzed to identify novel drug targets that could generate broad-spectrum, safe and effective therapeutic agents against Listeria monocytogenes infections in...
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