Posted by admin on Apr 30, 2023 in |
Biopolymers are gaining much interest and are considered appropriate substitutes for petroleum-based plastics. It is used for various applications in the medical, agriculture, and packaging fields. The bacterial strains from river soil samples were isolated and screened for biopolymer production. The objective of the present study was to produce polyhydroxybutyrate (PHB) from the isolated strains under optimized conditions in a shake flask. The optimization was done with the software tool Design expert – Response surface methodology (RSM). The optimum environmental conditions were: initial pH 7.0, Carbon Source 35g/l, and Nitrogen source 1g/l. Under these controlled conditions, biopolymer yield was obtained 2.25g/L. The FT-IR spectra of extracted biopolymer indicate symmetric (O-C-O) presence at 1600cm-1 and asymmetric absorption peak at 1400 cm-1. The TGA result indicates the thermal degradation of the obtained biopolymer was at 106 ℃. The obtained results suggest that these biopolymers may be similar to...
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Posted by admin on Apr 30, 2023 in |
In recent days, the increasing number of microbes and their increasing resistance power against conventional drugs have led to enormous worldwide mortalities, hence they pose a great threat to human health. The modern era is already going through the threat of COVID-19, also caused by one of those microbes called the virus. In order to get a clear understanding, all the microbes have been classified in certain types. Nowadays, to develop new alternative antimicrobial medicines, scientists must acquire clarity about the responsible functional groups of different conventional drugs with proper mechanistic elucidation on different types of microbes. This information not only clarifies the functionalities and properties responsible for exhibiting antimicrobial effects, but also facilitates the idea of new drug development through proper functional group incorporation or modification. These modifications increase the efficacy of antimicrobial drugs as well as their activity and water solubility. In this review, my focus will majorly be on the four main types of microbes and their possible mechanistic elucidation of commonly used antibiotics and alternative...
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Posted by admin on Apr 30, 2023 in |
Docking has become an indispensable method for predicting a molecule’s efficacy and toxicity. This technique is of great value in reducing the pain and suffering caused due to toxic drugs when tested in animals. Rheumatoid arthritis and the pain associated with the condition is a curse to mankind. In this research, the molecular property and toxicity predictions and evaluation of anti-arthritic and analgesic activities of L-Ascorbic acid derivatives were carried out using in-silico models. The molecular property and toxicity were predicted using the software called as OSIRIS. Molecular properties of compounds like ClogP, solubility, molecular weight and drug-likeness were evaluated. Toxicity parameters like mutagenicity, tumorigenicity, irritancy and reproductive effect were also examined. The anti-arthritic and analgesic activities were analyzed using CDOCKER. All the designed 10 molecules were docked with Hase enzyme for evaluation of anti-arthritic activity and opioid receptor for evaluation of analgesic activity. The results showed favourable ClogP, solubility, molecular weight, and drug-likeness values and excellent dock scores with toxicity profiles except for C4 and C6. Compound C10...
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Posted by admin on Apr 30, 2023 in |
A reverse phase high-performance liquid chromatographic bio analytical Technique (RP-HPLC) was developed and validated as per ICH 2019, US FDA 2018 guidelines for the quantification of amlodipine besylate, Rosuvastatin and Valsartan in human plasma using RP – C18 column. The mobile phase in composition (acetonitrile: water in the ratio 75:25% v/v, pH adjusted to 4.0 with acetic acid) was pumped and run at a flow rate of 0.8 ml/min, and for monitoring of eluents, a detector was set at wavelength 245 nm. The precipitation method was employed for the separation of the analyte from the plasma. A calibration curve was generated from the response and respective conc of standard solutions in the Concrange of LLOQ to ULOQ. The method was studied and validated per regulatory guidelines for selectivity, specificity, accuracy, precision, and stability study. Statistical data of the calibration curve of these drugs in the biological matrix was found within the prescribed limit. Results of accuracy and precision of quality control samples of these combined drugs were found (104.25...
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Posted by admin on Apr 30, 2023 in |
The analgesic activity of vilazodone in albino rats and mice was compared with the standard drug pentazocine and diclofenac, and its anti-inflammatory activity in albino rats was compared with the standard drug indomethacin. The doses of vilazodone used were 1.8mg/kg and 3.6mg/kg in albino rats and 2.6mg/kg and 5.2 mg/kg in albino mice, and the methods to screen analgesic activities were eddy’s hot plate method, tail flick method using radiant heat and acetic acid induced writhing method and formalin-induced peritonitis was used to screen anti-inflammatory activity. The data was analyzed statistically and results were expressed as numbers and percentages. For vilazodone dose of 1.8mg/kg and 3.6mg/kg using eddy’s hot plate method mean reaction time were 6.50± 0.288 and 5.70± 0.285, in tail flick method 6.50±0.288 and 6.45± 0.521. In the writhing method at a dose of 2.6mg/kg and 5.2 mg/kg in albino mice vilazodone showed 30.33±1.15 and 28.16±0.98 analgesic activity compared to control. In formalin induced peritonitis vilazodone 1.8mg/kg and 3.6mg/kg showed 2.2±0.13 and 1.86±0.05 anti-inflammatory activity when compared...
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