Posted by admin on Feb 28, 2017 in |
Herbal medicine is the oldest form of health care known to humanity and has been used in all cultures throughout history. The validation of these novel products needs powerful analytical devices tailored for the study of herbal extracts in order to assess composition and face their natural complexity as a resource. The last item is important and crucial for the capacity and utility of the analytical results that means that each product should be analyzed with the right approach. Having in mind these arguments, the present study was selected HPTLC as useful tool for the analysis. The present study is mainly focused to establish the fingerprint profile of Tragia plukenetii R. Smith using high performance thin layer chromatography (HPTLC) and Preliminary phytochemical screening. Preliminary phytochemical screening of the extract showed the presence of alkaloids, triterpenes, phenolic compounds and steroids. HPTLC finger printing of chloroform extract of leaf revealed 15 peaks with Rf values in the range of 0.11 to 1.0. It can be concluded that HPTLC fingerprint analysis of...
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Posted by admin on Feb 28, 2017 in |
The glutathione S-transferase (GST) isoenzyme superfamilies detoxify a wide-range of toxic chemicals and environmental substances are extensively expressed in mammalian tissues. Liver and pancreas are the sites where cytosolic Phase I and phase II biotransformation GSTs enzymes have characteristic expression. GSTs play a key role in the deactivation of reactive oxygen species (ROS) and the metabolism of lipids, chemotherapeutic agents. GSTs are mainly involved in conjugation of reduced glutathione (GSH) with diverse substrates specificity and it is possible that genetic variations in these enzymes will influence cellular response to the environmental agents. GSTs are overexpressed in response to a chemical or oxidative stress as an adaptive physiology and upregulated in cancerous state of organ or tissue. GSTs are essentially involved in susceptibility to various forms of cancer as they are vital in detoxification mechanism to metabolize the environmental carcinogens. GSTM1 encodes for a class mu GST isoenzyme involved in polycyclic aromatic hydrocarbons (PAHs) detoxification. The substrates of GSTM1 include benzo(a)pyrene, benzo(c)phenanthrene, benzo(g)chrysene and other carcinogens. They can catalyze in-vitro...
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Posted by admin on Feb 28, 2017 in |
Objectives: The study aimed at evaluating the broncho-relaxant effect of the test drug Flupirtine in experimentally induced Asthma in Guinea pigs & compares its efficacy with the Standard. Materials and Methods: Guinea pigs of both sexes weighing around 350-400 gm were randomly divided into 3 groups of 6 animals each. Groups were categorised into Control, Standard and test groups. Animals serving as controls received 10 ml/kg body weight of Normal saline; Standard group received 4.5 mg/kg body weight of salbutamol & the test group received the test drug flupirtine in doses of 46.5 mg/kg of body weight. All the compounds were administered once a day, per orally, through a gavage tube for 5 days. On day 5 experimental bronchial asthma was induced by exposing animals to 0.25% histamine acid phosphate under constant pressure (40mm/Hg) in an aerosol chamber. Animals exposed to histamine aerosol developed progressive dyspnoea. Pre-convulsive time (PCT) was determined from the time of exposure to onset of dyspnoea, immediately before the appearance of convulsions. Greater the PCT...
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Posted by admin on Feb 28, 2017 in |
The purpose of the present investigation is to formulate and evaluate Lercanidipine pulsatile drug delivery system by press coating method. The inner core tablets were prepared by using direct compression method by using cross-carmellose sodium (Ac-Di-Sol), sodium starch glycolate, crospovidone as super disintegrants. The drug polymer interaction was investigated by FTIR and their results directed further course of formulation. The inner core tablets were evaluated for various post compression parameters like Tablet hardness, Friability, Weight variation, Drug content and in vitro dissolution for both core and coated Tablets. The disintegration time for the core tablet was found to be 35sec and the percentage drug release for the optimized formulation (F7) was 96.1% in 30mins. The Press coat tablets containing HPMC and Ethyl cellulose as a barrier layer. The press coat tablets were evaluated for post compression parameters and were found to be in limits. The percentage drug release of optimized formulation Ethyl cellulose (F7P3) was found to 99.65% at the end of...
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Posted by admin on Feb 28, 2017 in |
The kinetics and mechanism of oxidation of Phenylephrine (PHE) by Chloramine-T (CAT) in acid media have been investigated by spectroscopic technique at the temperature of 298 K. It is found that the reaction is of first order with respect to PHE, fractional order with respect to CAT & inverse order with respect to H+. The reaction rate remained unchanged with the variation of ionic strength of the medium indicating that the non ionic species are involved in the rate determining step. The rate of reaction decreases with increase in concentration of dielectric constant. Addition of chloride ions had no effect on rate of reaction. The stoichiometric ratio was found to be 1:1(PHE: CAT) ratio. The major oxidation product of PHE has been identified by an UV, Cyclic voltammetry (CV), IR, H1 NMR and Mass spectroscopy. On the basis of the experimental results, a suitable mechanism has been proposed. The rate constants of the rate-determining step together with the activation parameters were evaluated and also thermodynamic quantities were...
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