Posted by admin on Sep 30, 2017 in |
A simple, rapid and sensitive method using an isocratic Liquid chromatography coupled with Tandem mass spectrometry was developed and validated for the assay of nitrofurantoin in the Human Plasma. The Mass transition of nitrofurantoin and losartan (Internal standard) were M/z 237.1/151.9 and M/Z 421.300/ 179 in ESI Negative ionization. Linearity was observed between the nitrofurantoin concentration and the peak area ratio from 10.451 to 1033.897 ng/mL with R2 value of 0.99. Plasma samples containing nitrofurantoin were extracted with Acetonitrile: 5mM Ammonium Acetate (pH 3.8) (80:20). The observed recovery of nitrofurantoin was 90%. The intra-day and inter-day accuracy was performed. Stability parameter was performed. The method will be used in the determination of the pharmacokinetic parameters of nitrofurantoin....
Read More
Posted by admin on Sep 30, 2017 in |
Lead induced oxidative stress implies morphological dysfunctions and physiological deformation in the liver. The aim of present study was to establish the protective effect of chloroform extract of Murraya koenigii leaves against the lethal effects of lead on the antioxidant status of mice. Murraya koenigii Chloroform Extract (MKCE) was prepared by maceration. Male Swiss albino mice were divided into three groups of six each. Group I (Control group) served as normal diet and water ad libitum, Group II (lead treated group) received an intraperitoneal injection of lead acetate (15mg/kg) daily once whereas Group III (MKCE with lead-treated group) received MKCE (50 mg/kg) orally along with a single of injection of lead acetate intraperitoneally (15mg/kg) daily once. Experimental study was continued for the consecutive seven days. On the 8th day, liver and kidneys were desiccated; homogenized in normal saline to collect supernatant into vials for estimating serum malondialdehyde, antioxidant enzymes like superoxide dismutase, catalase, reduced glutathione, glutathione peroxidase, glutathione-s-transferase and ferric reducing ability of plasma levels. It was observed that...
Read More
Posted by admin on Sep 30, 2017 in |
Algae are extensively used in cosmetics, supplements and pharmaceutical industries. Researches support their hypotensive, cytotoxic, anti-inflammatory and many other biologic effects. In the present study, the cytotoxic, antioxidant and acetylcholinesterase inhibitory activities of two brown algae, Padina tetrastromatica and P. australis, have been evaluated. Total extract was prepared using methanol 80%. The extracts were evaluated for antioxidant and acetylcholinesterase inhibitory activity and cytotoxic properties against A-549, HT-29, HepG-2 and MCF-7 cell lines through DPPH, Ellman and MTT assays, respectively. The results revealed that P. australis with the IC50 value of 163.9μg/mL demonstrated stronger antioxidant activity compared to P. tetrastromatica (IC50 259.1μg/mL) in DPPH assay. Neither of the total extracts showed cytotoxic activity up to the maximum amount examined (100μg/mL). The total extracts of P. australis and P. tetrastromatica in concentration of 300μg/mL presented 15.6% and 23.7% enzyme inhibition in Ellman assay, respectively. It was concluded that the two algae have shown weak antioxidant and acetylcholinesterase inhibitory activity and their further use in prevention and treatment of diseases needs more...
Read More
Posted by admin on Sep 30, 2017 in |
Nanotechnology is an innovative and escalating field that has made its contribution to all spheres of human life. Conventional synthesis of nanoparticles suffers from serious problems like high temperature, poor yield, longer reaction time, and use of toxic and highly sensitive compounds. Green synthesis of nanoparticles is an emerging field which is known to eliminate / minimize the aforementioned drawbacks. In the present investigation, stable silver nanoparticles were synthesized in good yield by chemical reduction of silver nitrate in ionic liquids such as [BMIM] BF4 (IL-1), [BMIM] PF6 (IL-2), [BEMIM] BF4 (IL-3) and [BEMIM] PF6 (IL-4) at room temperature. X-ray diffraction (XRD) and scanning electron microscopy (SEM) have been employed for the characterization of structure and morphology of the synthesized silver nanoparticles. The as synthesized nanoparticles and pure ionic liquids were screened for their anti-microbial activities and it was found that both the pure ILs and silver nanoparticles possess significant antimicrobial...
Read More
Posted by admin on Sep 30, 2017 in |
The aim of the present study was to formulate topical microsponge-based delivery system containing ketoconazole for controlled release of the drug for proficient treatment of fungal infections. Ketoconazole loaded microsponges were prepared by quasi emulsion solvent diffusion method using ethyl cellulose N22 polymer with varied drug polymer ratios. The prepared microsponges were characterized by SEM, FTIR, and evaluated for surface morphology, % drug loading, particle size, % entrapment efficiency and in vitro drug release. The effect of formulation variables such as drug to polymer ratio, stirring speed on the physical characteristics of microsponges was examined. Compatibility studies using UV and FTIR indicated that there is no chemical interaction between drug and polymers. SEM studies revealed that the prepared micro sponges were spherical and porous with a mean particle size of 82.25μm. The formulations were subjected to in vitro release studies for 8 hr which showed sustained release. Microsponges were further incorporated in to carbopol gel formulation for topical delivery. Prepared gel formulations were evaluated for physical parameters like pH,...
Read More
