Posted by admin on Jun 30, 2017 in |
Our previous study highlighted the antimicrobial activity evaluation of Nigella sativa seed oil extract that was obtained from supercritical fluid extraction using carbon dioxide (SCFE-CO2) on Gram positive pathogenic bacteria. The aim of this study was to formulate oil-in-water (o/w) emulsion of previous N. sativa seed oil extract and to investigate its antimicrobial activity against Streptococcus mutans and Streptococcus sanguis as the most common causative bacteria of dental caries. Oil-in-water emulsion containing 10% N. sativa seed oil extract (DLBS1355) exhibited good inhibition activity on both S. mutans and S. sanguis. Time-kill assay and biofilm inhibition assay were conducted using emulsion with 0.5% N. sativa seed oil extract content. The results showed bactericidal and biofilm inhibition activities on both tested bacteria. Antimicrobial activity of the emulsion was found higher on S. sanguis than S. mutans. Results of the present study showed that emulsification process did not significantly affect the antimicrobial activity of N. sativa seed oil extract and the formulated N. sativa oil-in-water emulsion could further be used as an...
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Posted by admin on Jun 30, 2017 in |
Typhoid fever and paratyphoid fever (also known as enteric fever, but collectively referred here as typhoid Fevers) are severe systemic illnesses characterized by sustained fever and abdominal symptoms. Treatment of typhoid fever becomes difficult by the development of multi-drug resistant of typhoid organisms especially to ampicillin, trimethoprim-sulfamethoxazole and chloramphenicol. In recent years, gradual development of resistance to fluoroquinolones has resulted in more challenges. Cefotaxime, ceftriaxone, Ciprofloxacin, Ofloxacin, Gatifloxacin, Co-trimoxazole, Ampicillin, Chloram-phenicol, Azithromycin can be used for the treatment with the antibiotic susceptibility test. But difficulties arise with the culture negative (i.e. unidentified febrile illness). A larger study enrolling 10153 patients in Bheri Zonal Hospital and Nepalgunj Medical College in western Nepal reported that 23.45% and 31.77% cases respectively were most prevalent during June-August season. An antibiotic susceptibility was also carried out in B P Koirala Institute of Health Sciences, Dharan eastern Nepal, reported that none of the isolates were resistant to ciprofloxacin. Researchers reported that in Patan hospital no characteristics clearly distinguished typhus patients from those with blood culture-positive...
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Posted by admin on Jun 30, 2017 in |
Background: A novel reconstitutable suspension of Paracetamol loaded microspheres was prepared using ethyl cellulose as a release controlling polymer and by solvent evaporation technique for the relief of osteo-arthritic pain. Objective: The formulation was developed to enhance patient compliance of population suffering from dysphagia or have difficulty in swallowing oral solid dosage forms like tablets and capsules. Method: Microspheres were evaluated for percent yield, drug loading, and entrapment efficiency, in vitro drug release profile and particle size. The microspheres were formulated as a dry oral suspension which has to be reconstituted at the time of use. Result: The particle size of microspheres was in the range of 550 -700 μm. The entrapment efficiency was in the range of 82.95- 95.56% and the yield obtained was 91.13-98.76%. The dissolution studies of selected trial showed a percent cumulative drug release of not less than 85% at the end of 3 hours. Formulation trial P5containing 4% xanthan gum, 5% purified talc and 6 mg sucralose gave a suspension having good flow property,...
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Posted by admin on Jun 30, 2017 in |
Plants have always been traditionally used to address various disorders. Due to their rich phytochemical profile, they have been employed in a gamut of skin care and concomitant treatment of disorders. In this Study, we screened the aqueous (CAE), hydroalcoholic (CHE) and ethanolic (CEE) extract of leaves of Cinnamomum verum for its activities The antioxidant potential of the extracts were determined by the DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Potential) assays. The extracts showed good activity with increasing concentration. Further, extracts were screened for anti-Inflammatory property by HRBC membrane stabilization assay and Inhibition of Protein Denaturation. The results of anti-inflammatory activity were compared with standard control values. CHE was found to exhibit better activities as compared to the other two extracts and was subsequently screened for Tyrosinase inhibition, Elastase inhibition and Hyaluronidase inhibition activity. CHE showed moderate anti-inflammatory and Hyaluronidase-inhibition activity. CHE can therefore be used as a potential bioactive component in skin care...
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Posted by admin on Jun 30, 2017 in |
Sterility is the complete absence of any viable microorganism in a drug product. The Specification of sterility is unchanging and is independent of the kind of manufacturing Process. Sterilisation of the final product in its container closure system or aseptic manufacturing is followed. Under Terminal sterilisation usually it involves filling and sealing product containers under high quality Environmental conditions. Products are filled and sealed in this type of environment, so as to minimise the microbiological content of the in-process product and also to help ensure that the Subsequent sterilisation process is successful. The main complicating factors are the temperature and humidity of the cleanroom and the variations between people. The length of time will also depend upon the grade of the cleanroom. Critical Consumables are designed to provide protection for the head, body, hands and feet. In the process of establishing a system for garment selection, it is important to consider the broader aspects of cleanroom use. The main purpose of this study is to check the level of...
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