Posted by admin on Jul 31, 2025 in |
The aim of this study is to assess the antibacterial efficacy of the Datura stramonium seeds and leaves extracts against Mycobacterium kansasii. The antimicrobial experiments were performed using five doses (0.25µg/ml, 0.50µg/ml, 1µg/ml, 2µg/ml, 4µg/ml) of seeds and leaves extracts. The results showed notable inhibitory effects on Mycobacterium kansasii. The results indicated that the extract of D. stramonium exhibited action against all the tested bacterial isolates, with the level of activity varying depending upon the concentration. The hexane extract of seeds DSS-1H exhibited antibacterial activity, with mean zones of inhibition measuring 98.94±50.25.%, 120.98±56.92%, 120.499±69.60%, 125.752±133.18% and 89.494±180.63%, at the doses of 0.25, 0.50, 1, 2, and 4 µg/ml and while treating with DSS-2A the percent cell viability of the microbial cell was found to be 128.02±25.64%, 86.79±14.95%, 34.61±16.33%, 26.29±29.76% and 32.56±19.48% at the doses of 0.25, 0.50, 1, 2 and 4µg/ml, respectively, with incubation period of 48 hours. Similarly, the microbial cells were treated (0.25, 0.50,1,2,4 µg/ml) with DSS-3ET with incubation period of 48 hours, indicating a more substantial...
Read More
Posted by admin on May 31, 2025 in |
Antimicrobial resistance continues to cause antibiotics treatment failure and millions of deaths globally. Inhibition of a double-stranded binding protein-A enzyme (DsbA), machinery in bacteria resistance and pathogenesis mechanisms make the bacteria vulnerable to existing antibiotics, allowing restoration of antimicrobial efficacy. However, there is a gap in identifying bioactive and safe DsbA-inhibitors as a therapeutic restoration strategy. In this in-silico screening study, a co-crystallized E. coli-DsbA enzyme protein was used as the target. Seven bioactive ligands: linoleic acid, aloesin, azadirachtin, nimbin, palmitic acid, stearic acid, and aloeresin-A, showed maximum docking scores of low binding affinity (kcal/mol): -6.8345, – 5.9434, – 7.2810, -6.2394, -6.5958, -6.7170 and -6.9554 respectively, lower than the standard (9AG): -4. 6919. The ligands obeyed Lipinski’s rule of 5. The Root Mean Square Deviation (RMSD) of E. coli-DsbA complexes with azadirachtin, and aloeresin-A displayed remarkable stability throughout the simulations. Molecular dynamics simulation on azadirachtin and aloeresin-A showed great docking scores and modes over 10000ps and revealed lowest binding affinity scores, remain stable in the active site. Additionally, astructural-activity...
Read More
Posted by admin on May 31, 2025 in |
The current study aims to find out how effective different extractions of Barleria cristata leaves are at fighting cancer, free radicals, and bacteria. Physical and chemical qualities and preliminary phytochemical analysis will also be assessed. It also quantifies several active ingredients by total phenolic and flavonoid. Five leaf extracts of Barleria cristata were made with ethyl acetate, chloroform, acetone, ethanol, and water. They were then tested for their antioxidant potential using reducing power and free radical scavenging methods, such as DPPH and NO radicals. They were also tested for antibacterial activity against Gram-positive and Gram-negative bacteria. The MTT assay measured how successfully the medication killed MCF-7 breast cancer cell line and Hep-G2 hepatoma cancer cell line. We calculated IC50. Initial phytochemical analysis was performed on all extracts. The total extract was abundant in protein, carbs, tannins, flavonoids, and phenolics. Acetone extract had higher phenolic and flavonoid components than ethyl acetate, chloroform, ethanol, and water. Barleria cristata acetone extract was antioxidant rich. Plant extracts also had antibacterial properties. Extracts inhibited...
Read More
Posted by admin on Apr 30, 2025 in |
Background: The emergence and spread of Vancomycin, Linezolid and combined linezolid/vancomycin resistance in Enterococcus species (LVRE) is a major therapeutic challenge. Vancomycin-resistant Enterococci are an important cause of healthcare-associated infections. Linezolid and Teicoplanin are currently approved for the treatment of VRE; however, resistance to these antimicrobials appears to be increasing. Aims/Objectives: The study was done to find the prevalence and to study the antimicrobial resistance pattern of Enterococci isolated from patients with bacteremia in a tertiary care hospital in Ahmedabad. Methodology: This retrospective study was conducted for a period of one year; Blood samples were received and incubated in Bact/Alert 3D automated blood culture machine. Positive indicated blood samples were cultured, and identification and antimicrobial susceptibility testing was done using both manual and automated methods. Results: In this study, 4147(35%) blood samples were found positive. Out of 4147 positive blood samples 278(6.7%) samples were shown growth with Enterococci species. Out of 278 Enterococci, 8(2.87%) Enterococci were found to have both linezolid and vancomycin resistance. Conclusions: Prevalence of LVRE is low...
Read More
Posted by admin on Apr 30, 2025 in |
Background & Objective: India is home to two infectious diseases caused by Salmonella: typhoid and paratyphoid fever. The medication of choice for S. typhi is ceftriaxone, a third-generation cephalosporin. Study was carried out on the Surveillance of Enteric Fever in India to ascertain the prevalence, clinical manifestation, and trajectory of antimicrobial resistance (AMR). This work aimed to examine the antibiogram profiles of Salmonella typhi found in patient blood cultures. Second, to record how often Salmonella typhi infections occur located in Vadodara, the Antibiotic Susceptibility Test is third. Material and Methods: The Retrospective study was carried out over a period of 12 months after obtaining ethical approval. The freshly drawn venous blood was placed in a blood culture vial. Following identification of pathogen isolates, Kirby-Bauer disc diffusion test was employed to determine antibiotic drug susceptibility as per CLSI rules. Results: Total 10,126 blood samples were collected. In 84 samples (0.83%), 83 instances of Salmonella typhi and 1 instance of Para typhi B. The ratio of males to females was 1:2....
Read More
