Posted by admin on Jan 31, 2021 in |
Rosuvastatin, a selective and competitive inhibitor of enzyme 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase, is marked as a first-choice drug amongst lipid-lowering agents because of its unique pharmacological and pharmacokinetic properties. An additional advantage of rosuvastatin administration is its specific, non-lipid pleiotropic properties, which are considered promising new fields of research for potential new therapeutic implications of this class of drugs. The aim of this study was to evaluate the antioxidant, anti-inflammatory, and anti-microbial activity of rosuvastatin with the application of the most commonly used in-vitro assays. Rosuvastatin was dissolved in dimethyl sulfoxide and prepared as a range of different concentrations. The activity of rosuvastatin was compared to well-known antioxidant, anti-inflammatory, and antimicrobial agents. For determination of antioxidant activity, a spectro-photometric DPPH radical scavenging assay was used, while inhibition of heat-induced protein denaturation was used for evaluation of anti-inflammatory activity of tested rosuvastatin solutions. Evaluation of antimicrobial activity was performed with the application of agar-well diffusion assay against both Gram-positive and Gram-negative bacteria as well as the fungi. The results of this...
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Posted by admin on Jan 31, 2021 in |
The antimicrobial activity of Urtica dioica L. and Urtica urens L. has been reported; however, there are no reports regarding Urtica mexicana Liebm., which is widely distributed in central Mexico. U. urens and U. mexicana were collected in Amecameca and U. dioica was collected in Toluca Valley, both located in the state of Mexico. Crushed leaves of the plants were soaked consecutively in hexane, dichloromethane, methanol and water. The minimum inhibitory concentration (MIC) was determined with 0.039 mg/mL to 5 mg/mL solutions of the extracts, which were added to cultures of Salmonella typhimurium, Shigella flexneri, Salmonella typhi, Escherichia coli, Proteus mirabilis, Bacillus subtilis, and Staphylococcus aureus (5 × 106 CFU/mL) with Resazurin sodium salt and Mueller‒Hinton agar. The lowest MIC (0.039 mg/mL) was observed for the hexane, dichloro-methane, and the methanol extracts of U. urens against Bacillus subtilis and the hexane and dichloromethane extracts of U. urens against S. aureus (0.156 mg/mL). U. mexicana showed a greater spectrum of activity at higher concentrations; U. dioica showed exhibited the least...
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Posted by admin on Dec 31, 2020 in |
In recent years, scientific research has confirmed the presence of large amounts of probiotic Lactobacillus sp. in fermented dairy products, which shows a positive impact on human health. This study was designed to isolate Lactobacillus with probiotic potential from local and commercial samples. 40 Lactobacilli strained were isolated from commercial and local yogurt samples by tenfold dilution plating method and plated on Man-Rogosa-Sharp agar medium and were screened for their use as potential probiotics. The isolates were tested for their ability to survive at pH 2.0, pH 3.0, in the presence of 0.3% bile salt and antimicrobial activity; sensitivity against 10 specific antibiotics (ciprofloxacin, erythromycin, tetracycline, penicillin G, ampicillin, streptomycin, polymyxin B, vancomycin, chloramphenicol and rifampicin) using filter paper disc diffusion method and two-fold serial dilution methods. Out of the 40 strains, 24 strains (42%) had survival rates above 90% after 2 h of incubation at pH values of 2.0 or 3.0 pH. Further screening performed on the above 40 isolates indicated that 24 strains show to 0.3% bile salt. Lactobacilli...
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Posted by admin on Dec 31, 2020 in |
The present study deals with the phytochemical analysis and evaluation of antibacterial activity of hydroalcohol, acetone, and hexane extracts of the leaves of Psidium guajava. The antimicrobial activity was measured by the agar well diffusion method. Gastric volume, pH, total and free acidity and the ulcerative index was also measured in hydroalcoholic extract of Psidium guajava on gastric ulcer pyloric ligation in rat. Glycosides, terpenoids, and tannins are only present in the hydro alcohol extract. The extracts are tested against gram-negative (Escherichia coli) and gram-positive (Staphylococcus aureus, Bacillus subtilis) bacterial strains. The zone of inhibition against microorganisms is direct proportional to the concentration of extract. Maximum zone of inhibition (24mm) against Staphylococcus aureus except hexane extract was seen. Psidium guajava leaves show maximum phytochemicals compounds and inhibition of microorganisms in hydroalcoholic extract. So the hydroalcoholic extraction was used for anti-ulcer activity. The present study was performed in pylorus ligation induced gastric ulcer model in albino rats in which the ability of hydroalcoholic extracts of Psidium guajava was tested at...
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Posted by admin on Dec 31, 2020 in |
Ruthenium(II) complexes of the type [Ru(bpy) 2(L)](PF6)2, where L= 2-{4,5-bis[(E)-2-phenylethenyl]-1H-imidazol-2-yl}-1H-benzimidazole (1), 2-{4,5-bis[(E)-2-(4-chlorophenyl)ethenyl]-1H-imidazol-2-yl}-1H-benzimi-dazole (2), 2-{4,5-bis [(E)-2-(4-fluorophenyl) ethenyl]-1H-imidazol-2-yl} -1H-benzimidazole (3), 2-{4 ,5-bis[(E)-2- (4-methylphenyl) ethenyl] -1H-imidazol-2-yl }-1H-benzimidazole (4), 2-{4, 5-bis[(E)-2-(4-methoxyphenyl) ethenyl]-1H-imidazol-2-yl}-1H-benzimidazole (5) have been synthesized and characterized by elemental analyses and spectral (IR, UV-vis, NMR, ESI-MS) techniques. The redox behavior of the complexes has been studied by cyclic and differential pulse voltammetry. In acetonitrile solution, all the complexes exhibit characteristic metal to ligand charge transfer (MLCT) absorptions and ligand-based transitions. The complexes showed efficient DNA cleavage activity in the presence of light at the wavelength of 480 nm. The complexes are also able to cleave supercoiled pUC19 plasmid DNA via guanine base oxidation in a concentration-dependent manner. In-vitro cytotoxic activity of the complexes shows that complex 1 has better anticancer activity against MCF7 human breast cancer cells with an IC50 value of 7.9 µM. The antimicrobial activities of the ligand and their metal complexes were screened by agar diffusion method and found that the metal complexes have higher antimicrobial activity than the...
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