Posted by admin on Feb 29, 2020 in |
The present study was carried out to evaluate phytochemical composition and pharmacological activities of leaf extract of Lusia zeylanica by using different solvents. The solvents like n-hexane, ethyl acetate and methanolic were used to extract dried leaf material of L. zeylanica. These extracts were analyzed for phytochemical constituents with GC-MS analysis and antimicrobial activity tested against four bacterial strains and three fungal strains by using the agar diffusion method. In-vitro anticancer activity was done against two cancer cell lines (MCF-7 and HeLa cell line) by using MTT assay. Phytochemical analysis revealed the presence of coumarins, flavonoids, glycosides, phenols, saponins, tannins, and terpenoids. GC-MS analysis determines the presence of 11 compounds in ethyl acetate, 7 compounds in methanolic extracts and a total of 7 unknown compounds respectively. A significant cancer cell growth inhibition was observed for two extracts with IC50 values ranging between 18.36 µg/ml to 67.914 µg/ml. Our result shows this plant is a promising source of phytocompounds with potential antimicrobial and anticancer...
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Posted by admin on Jan 31, 2020 in |
Bacteria have proved remarkably developing resistant to a known antibiotic; however, Medicinal plants have manifested notably useful for the control of bacterial infections. This study aimed to isolate and identify the multidrug-resistant bacteria from wound infection, then examine the antibacterial activity of different concentration of the ethanolic extracts of Cymbopogon proximus, Azadirachta indica, and Grewia senegalensis plants against it. One hundred specimens collected from wound infection, isolation and identification done according to standard methods. Antibiotics susceptibility test performed using the Kirby-Bauer disc diffusion method for: Cotrimoxazole (25 μg) Ciprofloxacin (5 μg), Gentamicin (10 μg), Imipenem (10 μg), Erythromycin (15 μg), Tetracycline (30 μg) Methacillin (5 μg), Ceftazidime (30 μg). 94 isolates showed bacterial growth, 40 (42.6%) isolates were Gram-positive bacteria (Staphylococcus aureus) and 54 sample Gram-negative bacteria; 15 (27.8%) Pseudomonas aeruginosa, 13 (24.1%) Proteus spp, 12 (22.2%) Escherichia coli, 10 (18.5%) Klebsiella pneumonia, 4 (7.4%) Citrobacter freundii. Ethanolic extracts from Sudanese medicinal plants namely Cymbopogon proximus, Azadirachta indica, and Grewia senegalensis, screened for their antimicrobial properties against isolated multidrug-resistant...
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Posted by admin on Jan 31, 2020 in |
Mupirocin is a topical antibiotic which is very effective against staphylococci, streptococci and Methicillin-Resistant Staphylococcus aureus (MRSA) infection. Because of its high effectiveness against MRSA infection, it is frequently used in clinical practice for decolonization of MRSA. Due to the increased use of mupirocin, increased resistance rates have been reported. Essential oil is a natural antimicrobial agent which kills the bacteria without promoting the acquisition of resistance. Hence, the combination of mupirocin with essential oils would be an ideal solution for preventing mupirocin resistance. Therefore, in this study we have investigated the antibacterial effect of mupirocin in combination with 3 essential oils (cinnamon, eugenol, and eucalyptus). Zone of inhibition test using disk diffusion method and Minimum Inhibitory Concentration (MIC) test using broth dilution method was carried out to assess the antibacterial activity against S. aureus. In this study, we have checked the type of interaction (synergistic, antagonistic, indifference, additive effect). From the results, it was observed that all the essential oil showed potential synergistic antibacterial effect against S. aureus....
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Posted by admin on Jan 31, 2020 in |
Plants are a valuable source of secondary metabolites. Many phytochemicals are obtained from the plant sources that are used as valuable medicinal agents. Chalcones are one of the important secondary metabolites obtained from many edible plants like foods, vegetables, tea, spices and natural foodstuffs. Chalcones considered as obligate intermediated in flavonoid biosynthesis but they do not accumulate to an appreciable degree in most plants. Chalcones are a group of plant-derived polyphenolic compounds belonging to the flavonoids family that possess a wide variety of cytoprotective and modulatory functions, which may have therapeutic potential for multiple diseases. The largest number of natural chalcones has been isolated from species of the Leguminosae, Asteraceae and Moraceae families. Chalcone accumulating plants have often been used in traditional medicine and These chalcones and their derivatives have shown important antimicrobial, antifungal, anti-mycobacterial, antimalarial, antiviral, anti-inflammatory, antioxidant, anti-tumor, antileishmanial, anticancer properties. The purpose of this review is to summarize the various naturally occurring chalcone compounds which have been isolated from different plants and to describe the recent...
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Posted by admin on Jan 31, 2020 in |
Antibiotics inhibit bacterial cell wall synthesis, protein synthesis or nucleic acid replication. The antibiotic must have access to and bind to its bacterial target site. Whether antibiotic resistance is intrinsic or acquired, the genetic determinants of resistance encode specific biochemical resistance mechanism that may include enzymatic inactivation of the drug, alteration to the structure of the antibiotic target site and changes that prevent access of an adequate concentration of the antimicrobial agents to the active site. Klebsiella organisms are often resistant to multiple antibiotics. Current evidence implicates plasmids as the primary source of the resistance genes. The susceptibility pattern of different antibiotics to ESBL producing Escherichia coli and Klebsiella spp. isolated from urine samples and they found that most of the infection caused by E. coli (61.29%) found to be most common bacteria in urinary tract infection followed by Klebsiella spp. (45.16%). Isolates were highly resistance to gentamicin followed by tetracycline, ampicillin, and amikacin. gentamicin showed resistance against E. coli and Klebsiella spp were 89.47% and 85.71% respectively. While tetracyclin and ampicillin showed...
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