Posted by admin on Jan 31, 2019 in |
This study aimed to introduce antibacterial nanofibers, produced by electrospinning as a novel technique in constructing nanostructured materials. In the electrospinning technique, a high voltage is applied to a polymer solution to overcome the surface tension. A charged polymer jet is ejected to a grounded electrode. As the solvent is evaporated, the electrospun fiber with diameter from micrometer to nanometer is formed. One of the most important features of electrospun nanofibers stated in this paper is their activity as effective carriers of antibacterial substances, which leads to the use of them in many fields such as food packaging, drug delivery, wound healing, filtration, and food sciences. An electrospun nanofiber containing antimicrobial agents indicates excellent antibacterial functions against a wide spectrum of bacteria and sustain the antibacterial activity over a long period. In general, several aspects of antibacterial nanofibers, including synthesize, applications, types of antibacterial agents combined with them such as essential oils, herbal bioactive components, metals, drugs, and peptides have been...
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Posted by admin on Jan 31, 2019 in |
Standardization is a process involving various chemical analysis methods based on pharmacological data involving general physical and microbiological analyzes aimed at the safety of a natural extract. To obtain the correct result, the standardization step is preceded by method validation. Quercetin can be obtained from the leaves of soursop (Annona muricata Linn.), which use for treatment fever, diarrhea, anti-convulsive, antifungal, antimicrobial, gout, itching, and flu. Determination of quercetin in soursop leaf extract using TLC-Densitometry must be validated by testing selectivity, limit of detection (LOD), limit of quantification (LOQ), linearity, precision, accuracy, and range. Densitometry scanning of the plates silica gel 60 F254 directly at 265 nm was used for analysis of quercetin. The result of selectivity test was 1.53 (>1.5) with mobile phase toluene: ethyl acetate: formic acid (4: 3: 0.4; v/v/v), limit of detection (LOD = 0.018 µg), limit of quantification (LOQ = 0.060 µg), linearity y = 9387.3x – 767.39 (r = 0.9996 and Vxo = 1.9%), Coefficient of Variation (C.V.) of precision 1.906525% and the percent...
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Posted by admin on Jan 31, 2019 in |
Plants are an important source for the discovery of new products of medicinal value for drug development and plants secondary metabolites are unique sources for pharmaceuticals food additives, flavors, and other industrial values. The commercial importance of these secondary metabolites has resulted in a great interest in its production and in exploring possibilities of enhancing its production using tissue culture technology in recent years. A good number of abstracts and research articles published, so far, for evaluating antioxidant, the anti-microbial and anti-diabetic activity of different secondary metabolites which have been extracted from various plants. In this article, we reviewed bioactivity of flavonoids, alkaloids, and phytosterols of total 18 different plants. Out of which, total of 10 plants were found to show antioxidant potential, 9 plants were found to show anti-microbial potential, and 9 plants were found to show the anti-diabetic potential of secondary metabolites. Root, stem, leaves, fruits, and flowers of various plants were found to possess secondary metabolites to show bioactivity. So this article will be a comprehensive...
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Posted by admin on Jan 31, 2019 in |
The present study was carried out to assess the various phytochemical composition, GC-MS analysis, and antimicrobial potential of methanolic leaf extract of Alstonia scholaris (L.) R. Br. by using different solvents. Phytochemical analysis revealed the presence of alkaloids, coumarins, flavonoids, glycosides, phenols, quinines, saponins, tannins, steroids and terpenoids. Susceptibility testing by Agar well diffusion assay showed significant antimicrobial activity with ethyl acetate and methanol extracts of leaves against bacterial strains such as Bacillus subtilis, Bacillus cereus, Pseudomonas aeruiginosa, Staphylococcus aureus, Escherichia coli and fungal strain Candida albicans. The methanolic leaf extract exhibited better antimicrobial activity than ethyl acetate extract. The UV-Vis, FTIR, and GC-MS have been employed to characterize the bioactive compounds present in methanolic leaf extract of Alstonia scholaris. The crude extract was scanned in the wavelength ranging from 200-900 nm by using Perkin Elmer Spectrophotometer, and the characteristic peaks were detected. The FTIR spectrum confirmed the presence of alcohol, alkanes, amides, alkyl aldehydes, halogen, and aromatic compounds. The results of the GC-MS analysis provide different peaks determining...
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Posted by admin on Jan 31, 2019 in |
The rhizomes of Curcuma caesia Roxb. (Zingiberaceae) Are traditionally used in the treatment of various ailments and metabolic disorders like leucoderma, asthma, tumors, piles, bronchitis, etc. in Indian system of medicine. Considering the importance of natural products in modern phytomedicine, the antioxidant and ant mutagenic activities of C. caesia Roxb. rhizome extract and its fractions were evaluated. The antioxidant results indicate that the free radical-scavenging activity may be attributed to the high contents of phenolics and flavonoids with a higher reducing capacity. The IC50 values of in-vitro and ex-vivo antioxidant activities of Curcuma caesia was found for DPPH scavenging activity was 1.08 ± 0.06 μg/ml, nitric oxide scavenging activity was 37.50 ± 1.54 μg/ml, Hydroxyl radical scavenging activity was 10.08 ± 2.01 μg/ml, Superoxide Anion scavenging activity was 29.63 ± 2.07 μg/ml respectively. The in-vitro antimicrobial activity of different fractions obtained from the rhizome of Curcuma caesia was investigated against standard strain and clinical isolates of Staphylococcus aureus. The clinical isolates were found more sensitive for different fractions, than...
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