Posted by admin on Nov 30, 2015 in |
Sugarcane is the major cash crop of the Indian tropical and sub-tropical region. Mostly the foremost part of Indian economy is based on sugarcane crop, which is cultivated major state in India. Phyto-components are the compound that occurred in plant naturally and play important role for biologically activity (Anti fungal, Anti cancer Anti diabetic etc), to prevent the many disease, scavenging and chelating the free radicals. In the present study we identified the phytocomponent presence in juice sample of Mungo 254 (Saccharum barberi) through GC-MS analysis. The major compound sucrose (30.64%) with retention time 12.18 and lower percentage compound was Pentanal, 2-methyl (0.10%) with 6.48 retention time. The identified compounds having biological and pharmacological activity such as Antimicrobial, Antifungal, Anticancer, Antioxidant, Antimutagenic and Hypercholesterolemic...
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Posted by admin on Oct 31, 2015 in |
β-lactams have been widely used as antibiotics for treatment of nosocomial and community acquired infections for the last five decades. Under selective pressure from the extensive use of cephalosporins in 1980s and 1990s, many bacteria have emerged as resistant against these antibiotics. The resistance to β-lactam antibiotics can be due to any of the following three mechanisms i.e. decreased accumulation of the drugs by bacterial cell, hydrolysis of the antibiotics by β-lactamases (βL) and alterations in penicillin binding proteins that reduce their affinity for the drug. WHO has repeatedly warned for a growing emergence of bacterial antibiotic resistance. The consequences of drug resistance include higher mortality and morbidity. Hence there is a need to search for new alternative antimicrobial agents with fewer side effects. In the current study, we have checked the antimicrobial and antioxidant activities of ten traditionally used medicinal plants against β-lactam resistant bacteria isolated from urinary tract infected (UTI) patients. We have also proposed phytochemicals extracted from medicinal plants as potential antibacterials and investigation of inhibition...
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Posted by admin on Oct 31, 2015 in |
Introduction: Bloodstream infections (BSI) are a serious cause of morbidity and mortality worldwide. Emerging antimicrobial drug resistance among bacterial pathogens causing BSI can limit therapeutic options and complicate patient management. Objective: To encourage the prudent use of appropriate antibiotics in our tertiary care Hospital, we studied the prevalence and antibiogram patterns of blood culture isolates from March 2013 to February 2014. Results: Of 829 blood cultures examined, 116 (13.9 %) were positive for bacterial growth. The frequency of Gram-positive bacteria isolated was 52.5% (61 of 829) and that for Gram-negatives was 47.4% (55 of 829). The most common gram-positive organism isolated was Staphylococcus aureus (28.5%), followed by CoNS (13.3%) and Enterococcus spp. (5.7%). Staphylococcus aureus, had exhibited least resistance to tetracycline, doxycycline, vancomycin, daptomycin, and linezolid. The rates of methicillin (oxacillin) resistance in Staphylococcus aureus were 32%. Among the Gram-negative isolates, the predominant isolates were Acinetobacter and Salmonella typhi (36.3%) followed by Escherichia coli (10.9%), Klebsiella species (9%) and Pseudomonas species (7.27%). ESBL and MBL production was seen in...
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Posted by admin on Oct 31, 2015 in |
Rapanea ferruginea Mez. stem barks and fruits extracts have demonstrated anticholinesterasic and antimicrobial activities assigned to myrsinoic acids A and B (MAA and MAB), compounds that have also proven anti-inflammatory and antinociceptive activities. To stablish the quality control of nanotechnology-based herbal medicines, the aim of this study was develop and validate a stability indicating HPLC–DAD method for quantitative analysis of MAA and MAB markers in R. ferruginea stem barks and fruits extracts and nanoemulsions. Chromatographic separation was conducted on a Kinetex®C18 column (150 x 4.6 mm x 2.6 µm), with mobile phase consisted of acetonitrile: methanol: water (acidified with H3PO4 pH 2.50) and eluted in gradient mode with flow rate of 0.9 mL min-1, with 30 min of analysis and detection at 260 nm and 270 nm. The method was specific, linear, sensitive, accurate and robust for both markers. The chromatograms of stem barks and fruits extracts submitted to forced degradation study (acid, oxidative, UV and visible light) did not show impurities interference in MAA and MAB quantification, therefore,...
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Posted by admin on Oct 31, 2015 in |
Background: Hepatic encephalopathy (HE) is a brain dysfunction caused by liver insufficiency and/or portosystemic shunting. It is associated with poor survival and high risk of recurrence along with reduced quality of life (QOL) of patients and their caregivers. Nitazoxanide (NTZ) is an oral antimicrobial that improved mental status and QOL score in HE patients and is well-tolerated. Aim: To evaluate the efficacy and safety of NTZ compared to metronidazole and rifaximin in patients with grade II-III HE and to evaluate its effect on patients’ QOL. Patients and Methods: A Prospective, Randomized, Controlled, Open-Label, Pilot study. Thirty four patients were randomly assigned to receive either Nitazoxanide (n=12), Metronidazole (n=11) or Rifaximin (n=11) for 7 days. Serum ammonia level, Clinical Hepatic Encephalopathy Staging Scale (CHESS) and Chronic Liver Disease Questionnaire (CLDQ) for QOL was measured at baseline and at end of treatment. Results: Baseline and after 1 week serum ammonia levels and CHESS scores, showed no significant difference among the 3 groups. There was no significant difference in serum ammonia level...
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