Posted by admin on Aug 31, 2015 in |
The aim of the present study was to investigate the antifungal potential of trypsin inhibitors (AMTI-I and AMTI-II) isolated from the seeds of Abelmoschus moschatus on selected pathogenic fungal strains. The purified inhibitors have been found to be homogenous by the criteria of native PAGE and gel filtration with apparent molecular weights of 22.4kDa and 21.2 kDa as judged by SDS-PAGE. While both the inhibitors were strongly active against bovine trypsin, they showed moderate effect on porcine elastase. AMTI-I and AMTI-II significantly affected the growth of Candida albicans, Candida tropicalis, Asperigillus flavus, Saccharomyces cerevisiae, Candida glabrata and Asperigillus niger with notable zones of inhibition. The inhibitors, however, did not show any inhibitory effect on the growth of other fungal strains- Fusarium oxysporum, Alternaria alternate, Mucor indicus and Penicillium chrysogenum. The fungicides, Flucanazole and Ketoconazole were used as positive controls in this study. Results obtained suggest that AMTI-I and AMTI-II may be regarded as excellent candidates for the development of novel antimicrobial agents against human pathogenic...
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Posted by admin on Jul 31, 2015 in |
The mouth of human body provides non-shedding surfaces (teeth) for natural microbial colonization. This can result in the accumulation of large masses of bacteria and their products at stagnant sites. Dental plaque, Periodontitis and gingivitis are some of this type of conditions, which can develop due to microbial accumulation on teeth and Infections of throat leading to tonsillitis, pharyngitis, laryngitis, sour throat etc. Chlorhexidine is widely used antimicrobial drug in the treatment of dental plaque and gingivitis and Flurbiprofen is used as anti-inflammatory drug to reduce pain and inflammation. Lozenges are designed to deliver medications directly to the mucus membranes of the mouth and oropharengial cavity by dissolving slowly when placed between the tongue and gums. Lozenges provide maximum amount of local action thus Chlorhexidine and Flurbiprofen are formulated as lozenges to provide local antiseptic and anti-inflammatory action. The candy based lozenges were prepared by heat and congealing method by sugar as lozenge base, HPC, HPMC as polymers, citric acid artificial flavours and colours and other essential excipients. Some...
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Posted by admin on Jul 1, 2015 in |
Wound is unpreventable consequences of life, which originates due to physical damage, chemical injury and microbial pathogenic infections, which extend to loss of cellular and functional continuity of living tissue. Calotropis gigantea is a weed plant it used as a wound healing agent. Calotropis gigantea is a waste land weed. Antioxidants represent a significant function to assist human, against infections and chronic diseases. The exhibit investigation has been contained to assess the phytochemicals and invitro antioxidant activity of Calotropis gigantea. Phytochemicals were analyzed qualitatively and the result sustained the presence of alkaloids, phenols, saponin, and steroids. The invitro antioxidant activity of root was investigated by DPPH (1, 1-Diphenyl-2-picrylhydrazyl) and Super oxide free radical scavenging activity method. In both methods, plant extract possess high antioxidant activity when equated with standard ascorbic acid due to presence of high capacity of various phytochemicals. The pro-wound healing activity of the Calotropis gigantea extract may be due to its high content of glycosides, flavonoids, phenolic compounds, and triterpenoids with antimicrobial and antioxidant properties. From...
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Posted by admin on Jun 30, 2015 in |
Yogaraja guggulu a poly herbal formulation, consisting of 29 ingredients, has been used for the neurological, musculoskeletal disorders and in general practice, used for osteo arthritis. In vitro antiarthritic activity is done against pathogens which also include septic arthritis causing microorganisms. This study focused on determining antimicrobial efficacies of methanol (A), ethylacetate (B) and hexane extracts (C) of the Yogaraja guggulu by measuring the zone of inhibition. In vitro antioxidant activities were also assayed by 1, 1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. Antimicrobial activity was tested against the pathogenic bacteria: Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Bacillus megaterium, Klebsiella pneumonia, Enterobacter aerogenes, Enterococci bacteria; Pathogenic fungi: Candida albicans, Candida tropicalis, Candida bombi, Candida utilis and Trichophyton rubrum. In these three extracts methanol extract (A) showed better zone of inhibition (19-50 mm) when compared with ethyl acetate (B) and hexane (C) (9-40 mm) and (9-45 mm) respectively, against the tested pathogens. DPPH antioxidant activity of all the three extracts showed potent radical scavenging activity in comparison with the positive control,...
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Posted by admin on Jun 30, 2015 in |
Turmeric is a spice obtained from the dried rhizomes of Curcuma longa, family Zingiberaceae. It has been extensively used in traditional Indian (Ayurveda) and Chinese medicine for various ailments such as anti-inflammatory, blood purifier and even as a cosmetic. Curcumin, the chief constituent in turmeric has been isolated centuries ago, has been found to have a wide range of pharmacological actions such as antioxidant, anti-inflammatory, anticancer, antimicrobial and much more. Thus curcumin and its analogues have immense therapeutic potential for use in rheumatoid arthritis, Crohn’s disease, cancer, diabetes, cardiovascular disease, HIV-I and Alzheimer’s disease. Phase I clinical studies have shown that high doses of curcumin is well tolerated in man. Despite its safety, curcumin still evades clinical use due to poor bioavailability. Novel formulations of curcumin with piperine, soluble fibres of fenugreek, liposomes, micelles, nanoparticles, cyclodextrin and turmerone have shown enhanced bioavailability to some extent, each one having its own limitations. Recent formulation of curcuminoid with 45% turmerone seems to be promising. Further research in this direction is imperative...
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