Posted by admin on Mar 1, 2014 in |
Background: Bacterial resistance to antimicrobial agents is on the rise and this is causing serious complications resulting in increased morbidity and mortality of bacterial infections. There is a need for new antimicrobial molecules in order to fight against Multi-Drug-Resistant Organisms. The Mediterranean area is rich in a variety of medicinal plants and this may represent a potential for new compounds and molecules with enhanced antibacterial activity. Methods: The antimicrobial effects of three traditionally used Lebanese plants were investigated against 24 clinical isolates of Staphylococcus aureus with different phenotypes of resistance. Rosmarinus officinalis, Rheum rhaponticum, and Origanum majorana where extracted with ethanol, then further subfractionated with petroleum ether, dichloromethane and ethyl acetate. The remaining aqueous fraction was also collected, thus a total of five extracts were studied for each plant. The MIC and MBC of these extracts were determined using the micro-dilution technique. Results: Rosmarinus officinalis was the most effective against most of the strains studied including MRSA, QS, QR, MS and MR. The ethyl acetate fraction of Rosmarinus officinalis, Rheum...
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Posted by admin on Mar 1, 2014 in |
The study was carried out to assess the antibacterial & cytotoxic activities of extracts from leaf parts of the plant, Averrhoa bilimbi. Ethanol, methanol and water were used as solvents and antibacterial & cytotoxic effects were measured using disc diffusion test & brine shrimp lethality bioassay (BSLB). The susceptibility of the microorganisms to the extracts of this plant was compared with standard antibiotic cefadroxil. Water extract exhibited more potent antimicrobial activity by inhibiting wide range of gram positive and gram negative bacteria in comparison to ethanolic and methanolic extract of leaves of A. bilimbi. On the other hand, the ethanolic extract of A. bilimbi leaves( EEL) was found to be the most toxic to Brine Shrimp nauplii, with LC50 of 3.7 μg/ml whereas anticancer drug vincristine sulfate (VS) proved LC50 value 1.73 μg/ml indicating that the potent cytotoxic compounds in this plant have affinity for non- polar solvents. The spectrum of activity observed in the present study may provide the indication that the plant could be a possible source...
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Posted by admin on Feb 1, 2014 in |
The objective of this study was to determine antibiotic susceptibility pattern of methicillin resistance Staphylococcus aureus in tertiary care center at Southern Rajasthan. Modified Kirby Baurer disc diffusion method was used for antimicrobial sensitivity testing of all samples. Maximum resistance among Staphylococcus aureus isolates (n=400) was observed against Methicillin (59%), Amikacin (53%) followed by other antimicrobial agents. Earlier Vancomycin, Linezolid and Pristinamycin were 100% sensitivity to MRSA. But results of this study shows that among MRSA isolates 13 % were found resistant to Vancomycin (VRSA), 12 % isolates found resistant to Linezolid and 9 % isolates Pristinamycin resistant. Pristinamycin was found sensitive in 75% of MRSA which were also Vancomycin resistant (VRSA) and Linezolid resistant (LRSA). The emergence of drug resistance and it’s dissemination in MRSA is worrisome. So we need to develop newer agents as well as slow down the spread of resistant strains by various...
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Posted by admin on Feb 1, 2014 in |
The genus Swietenia belongs to Meliaceae family which consists of 37 genera and about 300 species which is found in most of the parts of India. In present investigation we made an efficient effort to extract ricinoleic acid from the seed oil of Swietenia humilis, Zucc. They are used in medicine folklore to cure various diseases in Mexico, Africa and India. Physicochemical properties of Swietenia humilis Zucc seed oil were identified along with its fatty acid composition. The structure of the ricinoleic acid was confirmed by IR, NMR and Mass spectral studies. The extracted ricinoleic acid was screened for its antimicrobial and anthelmintic...
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Posted by admin on Feb 1, 2014 in |
1, 5-Dihydrobenzothiazepines are synthesized by conventional and microwave assisted synthesis methods. By microwave assisted synthesis, a considerable increase in the reaction rate has been observed and that too, with better yields. The compounds have been screened for antimicrobial and cytotoxic activity. 1, 5-Dihydrobenzothiazepines are prepared by the reaction of 1, 3-diarylprop-2-enones with o-aminothiophenol. All the products were tested for purity by tlc and characterized by elemental analysis, IR, 1H-NMR, 13C-NMR and mass spectral...
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