Posted by admin on Dec 31, 2020 in |
The rapid increase in multi-drug resistance (MDR) infections present a challenge in the development of therapies against them. Antimicrobial peptides (AMPs) can be the answer to this challenge. AMPs play an important role in host defense mechanisms. Plants are the precious source of natural antimicrobial molecules, including antimicrobial peptides known as Plant Antimicrobial Peptides (PAMPs). The present research work was aimed to isolate antimicrobial proteins or peptides from the leaf and flower extract of Bougainvillea ‘Texas King’ and Bougainvillea ‘Shubra’ cultivars. Protein pellets obtained after 50% and 25% ammonium sulfate precipitation of B. ‘Shubra’ leaf and flower extract showed maximum total protein of 311 µg/ml and 798 µg/ml respectively and 25% and 50% protein pellet of B. ‘Texas King’ leaf and flower extract showed maximum total protein of 529 µg/ml and 904 µg/ml respectively. Different protein pellets and supernatant were screened for antimicrobial activity against selected microorganisms. The protein samples having antimicrobial activity were electrophoresed on 12% Tricine SDS-PAGE to estimate the molecular masses of the antimicrobial protein/peptides. Molecular...
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Posted by admin on Dec 31, 2020 in |
The nanoclusters are having different properties than their bulk material. Hence, the synthesis and applications of nanoclusters of metal oxides have received more attention. Out of many available methods for the synthesis of metal oxide nanoclusters, the electro-chemical reduction method is simple, cheap, and provides pure material hence preferred. The present study describes the synthesis of nickel oxide nanoclusters by electrochemical reduction method wherein the nickel-metal sheet was used as a sacrificial anode while platinum was used as an inert cathode. The electrolysis was carried out at varied current density for 2 h in aqueous solutions using tetrabutylphosphonium bromide (TBPB) as a capping agent. The nanostructure of the synthesized nanoclusters has been analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transforms infrared spectrometer (FT-IR), and UV-visspectro-photometer. The nickel oxide nanoclusters were screened for their antimicrobial potential against gram-positive and gram-negative bacterial strains of Staphylococcus aureus and Escherichia coli and found to have a broad spectrum of...
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Posted by admin on Dec 31, 2020 in |
Essential oils from plants have been reported to have antibacterial activity. Essential oil is a mixture of many chemicals, and one or more chemicals in essential oil may have antibacterial activity. In our laboratory, essential oil from the leaves of Ocimum sanctum L. was found to have antibacterial activity against 18 human bacteria. GC-MS analysis of the Ocimum sanctum essential oil revealed the presence of 19 chemicals, and one of them was eugenol. In the present study, eugenol was found to have an antibacterial effect against 4 Gram-negative and 2 Gram-positive human pathogenic bacteria by minimal bactericidal concentration, minimal inhibitory concentration and disc-diffusion methods. The minimal bactericidal concentrations of eugenol were 0.96 mg/ml, 4.17 mg/ml, 16.6 mg/ml, 16.6 mg/ml, 33.3 mg /ml and 33.3 mg /ml against Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Serratia marcescens and MRSA, respectively. The minimal bactericidal concentrations and minimal inhibitory concentrations in Gram-negative bacteria Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Serratia marcescens were similar. The minimal inhibitory concentrations are more than...
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Posted by admin on Nov 30, 2020 in |
Urinary tract infection (UTI) is one of the common clinical conditions in patients presenting to the tertiary care hospital. In general, most of the case of UTI was being treated by empirical antimicrobial therapy before the laboratory results of urine culture; since the antimicrobial susceptibility test of uropathogenic bacteria and antibiotic resistance for enhanced management of UTI with a commonly used antibiotic. A prospective observational study was conducted on significant bacteria isolated from the urine samples submitted in microbiology unit from April 2017 to September 2017 at tertiary care hospital in Kathmandu, Nepal. E. coli (52.5%); the predominant etiological organism of UTI in our study showed high sensitivity to nitrofurantoin (93.3%), meropenem (92.9%), and colistin (82.3%). The most effective antimicrobial agent against Gram-positive Enterococcus species were linezolid (100%), vancomycin (100%) and nitrofurantoin (100%). A very high rate of resistance was seen against amikacin (75%), nalidixic acid (68.7%), and cotrimoxazole (63.9%) in Gram-negative bacilli isolates of E. coli. Escherichia coli, the most common uropathogens isolated more commonly from the female...
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Posted by admin on Oct 31, 2020 in |
1-{3-[2, 3dihydro-1H-imidazol-1-yl (8-hydroxyquinolin-7-yl) methyl] phenyl-l} ethanon, nitrogen and oxygen-based bi-dentate mannich base heterocyclic ligand synthesized and complexed with biologically significant transition metals like copper, cobalt, nickel, and zinc by the melt condensation three-component one-pot synthesis. All the synthesized potential compounds characterized by spectral methods by using NMR, IR, UV-visible mass, and elemental analysis. The antimicrobial analysis investigated for all the synthesized compounds using some bacterial and fungal cultures used by the disc diffusion method, and the activity proved in proportion with the standard drugs used as a reference. The main significant part of the work involved in mannich base reaction of one-pot synthesis three-component reactions prove better synthetic procedure and improved product yield. The ligand and the complexes of transition metals proved better microbial activities of biologically important and to prove the efficiency of the present work. Continuation of research work is to investigate significant heterocyclic molecules and transition metal complexes to prove potential microbial activities play an important role in pharmaceutical and biological...
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